Reversible pulmonary trunk banding III: Assessment of myocardial adaptive mechanisms—contribution of cell proliferation Maria C.D. Abduch, DVM, PhD,

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Reversible pulmonary trunk banding III: Assessment of myocardial adaptive mechanisms—contribution of cell proliferation Maria C.D. Abduch, DVM, PhD, Renato S. Assad, MD, PhD, Miguel Q. Rodriguez, MD, PhD, Acrisio S. Valente, MD, José L. Andrade, MD, PhD, Léa M.M. Demarchi, MD, PhD, Miguel B. Marcial, MD, PhD, Vera D. Aiello, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 133, Issue 6, Pages 1510-1516 (June 2007) DOI: 10.1016/j.jtcvs.2006.12.010 Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 RV estimated mass (g) during training days. RV, Right ventricle; C, control group; CS, continuous stimulated group; IS, intermittent stimulated group. The Journal of Thoracic and Cardiovascular Surgery 2007 133, 1510-1516DOI: (10.1016/j.jtcvs.2006.12.010) Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Bidimensional echocardiogram at baseline (A) and after 5 days of training (B) in a CS group goat. RV, right ventricle; LV, left ventricle; PE, pericardial effusion. The Journal of Thoracic and Cardiovascular Surgery 2007 133, 1510-1516DOI: (10.1016/j.jtcvs.2006.12.010) Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Photomicrographs of the RV myocardium showing Ki-67 immunostaining. A, Arrows indicate 2 labeled cardiomyocyte nuclei. B, Numerous positive nuclei from interstitial and vessel cells. Objective: 40×; counterstaining with Harry’s hematoxylin. RV, Right ventricle. The Journal of Thoracic and Cardiovascular Surgery 2007 133, 1510-1516DOI: (10.1016/j.jtcvs.2006.12.010) Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Mean and SE values of: (A) cardiomyocytes Ki-67-positive index (number of positive cells/2000 cardiomyocytes expressed as a percentage); (B) interstitial/vessel cells Ki-67-positive index (number of positive cells/2000 expressed as a percentage). RV, right ventricle; LV, left ventricle; RVS, right half of ventricular septum; LVS, left half of ventricular septum. The Journal of Thoracic and Cardiovascular Surgery 2007 133, 1510-1516DOI: (10.1016/j.jtcvs.2006.12.010) Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions