Avrum I. Gotlieb, MD, CM, Alan Rosenthal, PhD, Pedram Kazemian, BSc

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Presentation transcript:

Fibroblast growth factor 2 regulation of mitral valve interstitial cell repair in vitro Avrum I. Gotlieb, MD, CM, Alan Rosenthal, PhD, Pedram Kazemian, BSc The Journal of Thoracic and Cardiovascular Surgery Volume 124, Issue 3, Pages 591-597 (September 2002) DOI: 10.1067/mtc.2002.123812 Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 1 Phase-contrast photomicrographs of porcine MVICs grown in 5% FBS (A, C, E, G) and 10% FBS (B, D, F, H) along wound edge show patterns of migration of elongated cells at 2 (A, B), 4 (C, D), 24 (E, F), and 48 (G, H) hours after wounding. All wounds were initially the same size. A, B, C, D, and F show cells along one edge; E, G, and H show cells along both wound edges. Note cells with frontal lamellipodia and tail (C, D). Note single cells and groups of cells present in wound early (E, F), and note contacts being made between cell processes later (G, H), as wounds closed. Wounds in 10% FBS closed more rapidly. Bar indicates 100 μm. Magnification 50×. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 591-597DOI: (10.1067/mtc.2002.123812) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 2 Wounded cultures of porcine MVICs showing extent of wound closure at 24 hours in cultures incubated with standard M-199 and varying concentrations of FBS. Error bars represent SD. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 591-597DOI: (10.1067/mtc.2002.123812) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 3 Immunofluorescent photomicrographs of interstitial cells 24 hours after wounding stained for FGF-2. Cell nuclei are stained with propidium iodide. A, Within wound; B, at wound edge; C, within monolayer in unwounded area; D, in unwounded monolayer. Bar indicates 50 μm. Magnification 600×. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 591-597DOI: (10.1067/mtc.2002.123812) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 4 Immunofluorescent photomicrographs of interstitial cells stained for FGFR-1 at 24 hours after wounding. Cell nuclei are stained with SYTOX Green. A, Cells at wound edge; B, cells behind wound edge; C, cells within monolayer away from wound edge. Bar indicates 30 μm. Magnification 600×. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 591-597DOI: (10.1067/mtc.2002.123812) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 5 Experimental in vitro wounds of elongated MVICs incubated with (black bars) and without (hatched bars) antibody to FGF-2. Error bars represent SD. P =.20,.045, and.002 at 24, 48, and 72 hours. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 591-597DOI: (10.1067/mtc.2002.123812) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions