Volume 24, Issue 1, Pages 1-8 (March 2017)

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Volume 24, Issue 1, Pages 1-8 (March 2017) Protective effect of chamomile (Matricaria recutita L.) decoction extract against alcohol- induced injury in rat gastric mucosa  Mohamed-Amine Jabri, Nadhem Aissani, Haifa Tounsi, Mohsen Sakly, Lamjed Marzouki, Hichem Sebai  Pathophysiology  Volume 24, Issue 1, Pages 1-8 (March 2017) DOI: 10.1016/j.pathophys.2016.11.001 Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 1 Subacute effect of chamomile decoction extract (CDE), famotidine (FAM) and ascorbic acid (AA) on macroscopic changes induced by ethanol (EtOH). Rats were pre-treated with various doses of CDE (25, 50 and 100mg/kg, b.w., p.o.), FAM (20mg/kg, b.w., p.o.), AA (250mg/kg, b.w., p.o.) or bi-distilled water, challenged with a single oral administration of EtOH (4g/kg, b.w., p.o.) or NaCl 9‰ for one hour. Pathophysiology 2017 24, 1-8DOI: (10.1016/j.pathophys.2016.11.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 2 Subacute effect of chamomile decoction extract (CDE), famotidine (FAM) and ascorbic acid (AA) on histologic changes induced by ethanol (EtOH). Animals were pre-treated with various doses of CDE (25, 50 and 100mg/kg, b.w., p.o.), FAM (20mg/kg, b.w., p.o.), AA (250mg/kg, b.w., p.o.) or bi-distilled water, challenged with a single oral administration of EtOH (4g/kg, b.w., p.o.) or NaCl 9‰ for one hour. Pathophysiology 2017 24, 1-8DOI: (10.1016/j.pathophys.2016.11.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 3 Subacute effect of chamomile decoction extract (CDE), famotidine (FAM) and ascorbic acid (AA) on ethanol (EtOH)-induced changes in stomach mucosa SH- groups level. Animals were pre-treated with various doses of CDE (25, 50 and 100mg/kg, b.w., p.o.), FAM (20mg/kg, b.w., p.o.), AA (250mg/kg, b.w., p.o.) or bi-distilled water, challenged with a single oral administration of EtOH (4g/kg, b.w., p.o.) or NaCl 9‰ for one hour. Essays were carried out in triplicate. a: p<0.05 compared to control group, b: p<0.05 compared to EtOH group, c: p<0.05 compared to CDE-25 group and d: p<0.05 compared to CDE-50. Pathophysiology 2017 24, 1-8DOI: (10.1016/j.pathophys.2016.11.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 4 Subacute effect of chamomile decoction extract (CDE), famotidine (FAM) and ascorbic acid (AA) on ethanol (EtOH)-induced changes in stomach mucosa (A) and plasma (B) hydrogen peroxide levels. Animals were pre-treated with various doses of CDE (25, 50 and 100mg/kg, b.w., p.o.), FAM (20mg/kg, b.w., p.o.), AA (250mg/kg, b.w., p.o.) or bi-distilled water, challenged with a single oral administration of EtOH (4g/kg, b.w., p.o.) or NaCl 9‰ for one hour. Essays were carried out in triplicate. a: p<0.05 compared to control group, b: p<0.05 compared to EtOH group, c: p<0.05 compared to CDE-25 group and d: p<0.05 compared to CDE-50. Pathophysiology 2017 24, 1-8DOI: (10.1016/j.pathophys.2016.11.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 5 Subacute effect of chamomile decoction extract (CDE), famotidine (FAM) and ascorbic acid (AA) on ethanol (EtOH)-induced changes in stomach mucosa (A) and plasma (B) free iron levels. Animals were pre-treated with various doses of CDE (25, 50 and 100mg/kg, b.w., p.o.), FAM (20mg/kg, b.w., p.o.), AA (250mg/kg, b.w., p.o.) or bi-distilled water, challenged with a single oral administration of EtOH (4g/kg, b.w., p.o.) or NaCl 9‰ for one hour. Assays were carried out in triplicate. a: p<0.05 compared to control group, b: p<0.05 compared to EtOH group, c: p<0.05 compared to CDE-25 group and d: p<0.05 compared to CDE-50. Pathophysiology 2017 24, 1-8DOI: (10.1016/j.pathophys.2016.11.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 6 Subacute effect of chamomile decoction extract (CDE), famotidine (FAM) and ascorbic acid (AA) on ethanol (EtOH)-induced changes in stomach mucosa (A) and plasma (B) calcium levels. Animals were pre-treated with various doses of CDE (25, 50 and 100mg/kg, b.w., p.o.), FAM (20mg/kg, b.w., p.o.), AA (250mg/kg, b.w., p.o.) or bi-distilled water, challenged with a single oral administration of EtOH (4g/kg, b.w., p.o.) or NaCl 9‰ for one hour. Assays were carried out in triplicate. a: p<0.05 compared to control group, b: p<0.05 compared to EtOH group, c: p<0.05 compared to CDE-25 group and d: p<0.05 compared to CDE-50. Pathophysiology 2017 24, 1-8DOI: (10.1016/j.pathophys.2016.11.001) Copyright © 2016 Elsevier B.V. Terms and Conditions