Modification of the βIII-tubulin C-terminal tail does not affect tubulin polymerization or the cellular proliferation rate. Modification of the βIII-tubulin.

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Modification of the βIII-tubulin C-terminal tail does not affect tubulin polymerization or the cellular proliferation rate. Modification of the βIII-tubulin C-terminal tail does not affect tubulin polymerization or the cellular proliferation rate. Representative Western blot (A) and densitometric quantitation (B and C) of the level of polymerized total tubulin (α-tubulin) (B) and polymerized modified βIII-tubulin proteins (GFP) (C) in gene-edited NCI-H460 cells expressing either the full-length βIII-tubulin protein (ZB3) or truncated βIII-tubulin protein (ZB3Δ) compared with the parental cell line (H460), which endogenously expresses βIII-tubulin. Mean ± SEM of four independent experiments, no significant difference. S, soluble fraction; P, polymerized fraction. (D) Cell number for NCI-H460 cells expressing the full-length βIII-tubulin proteins (ZB31 and ZB34), truncated βIII-tubulin protein (ZB3Δ1 and ZB3Δ2), or the βIII-tubulin body with βI-tubulin C-terminal tail (ZB3/CB12 and ZB3/CB13) measured periodically over 96 h by trypan blue dye exclusion and cell counting. Amelia L Parker et al. LSA 2018;1:e201800059 © 2018 Parker et al.