Fig. 8. Therapeutic effects of the transplantation of hiPSC-EPO–producing cells on renal anemia in adenine-treated mice. Therapeutic effects of the transplantation.

Slides:

Advertisements

Similar presentations

Fig. 6. Transgenic expression of αLNNd and mag in dyW/dyW mice improves muscle function, increases body weight, and prolongs life span. Transgenic expression.

Advertisements

Fig. 2. LUM015 fluorescently labels tumor cells in mouse models of STS and breast cancer. LUM015 fluorescently labels tumor cells in mouse models of STS.

Fig. 1. IL-6 is associated with resistance to EGFR TKIs and is induced by stress hormones. IL-6 is associated with resistance to EGFR TKIs and is induced.

Fig. 6. AZD6738 induces DNA damage and apoptosis and exhibits antitumor efficacy in xenograft models of high-risk medulloblastoma and neuroblastoma. AZD6738.

Transfer of miR-223 during neutrophil-epithelial cell interactions

Fig. 4. Intramuscular injection of AAV9-Cas9/sgRNA-51 corrects dystrophin expression. Intramuscular injection of AAV9-Cas9/sgRNA-51 corrects dystrophin.

Fig. 5. Correlation of tail and long bone growth velocities with Cxm serum concentrations in mice. Correlation of tail and long bone growth velocities.

Neutrophil aggregation is selectively induced by PS+ platelets

Fig. 1. BCAS1 expression identifies newly generated oligodendrocytes.

Fig. 3. Obese IFN-γ−/− mice develop accelerated NAFLD with fibrosis.

INT-767 treatment prevents renal lipid accumulation in diabetic DBA/2J mice. INT-767 treatment prevents renal lipid accumulation in diabetic DBA/2J mice.

Fig. 1 Localized treatment of TNBC cancers kills tumor cells and minimizes the metastatic burden. Localized treatment of TNBC cancers kills tumor cells.

Fig. 8. In vivo suppression of MM by CMLD

Fig. 3. A circadian rhythm in fibroblast wound-healing response.

Fig. 4. Prion infectivity of skin samples from sCJD patients.

Fig. 1. NASH and fibrosis develop late in mice fed an HFD.

Fig. 4 Infection-induced CLV dysfunction is associated with decreased LMC coverage. Infection-induced CLV dysfunction is associated with decreased LMC.

Fig. 2. GPC3 expression in normal and tumor tissues.

TFE3 prevents diet‐induced obesity and metabolic syndrome

Fig. 5. Pharmacological JAK2 inhibition in vivo abrogates tumor-initiating potential after chemotherapy. Pharmacological JAK2 inhibition in vivo abrogates.

Fig. 3. Wisper controls CF behavior and survival.

Fig. 3. Fc fusion GDF15 molecules improve metabolic parameters in obese mice and obese cynomolgus monkeys. Fc fusion GDF15 molecules improve metabolic.

Fig. 5. Perturbations of AHR-STUB1-TF axis normalize the hyperthrombotic uremic phenotype without altering the bleeding risk. Perturbations of AHR-STUB1-TF.

Fig. 5. Vascularization of human liver seed grafts.

Fig. 5. Stimulation of EPO expression and secretion under hypoxic culture conditions. Stimulation of EPO expression and secretion under hypoxic culture.

Fig. 1. CD31 is present throughout the human renal vasculature.

Fig. 1. Serum HBsAg, HBcrAg, and HBeAg reduction in human patients treated with a single dose of ARC-520. Serum HBsAg, HBcrAg, and HBeAg reduction in human.

Fig. 2. Effects of SFN in mouse hepatocytes and in rat models of diet-induced glucose intolerance. Effects of SFN in mouse hepatocytes and in rat models.

Type 1 immunity drives metabolic disease but protects against NAFLD

Fig. 2 In vitro assessment of hESC-RPE cell sheets.

Fig. 2 STED microscopy of isolated cardiomyocytes from mice treated with MP-rhodamine–loaded CaPs. STED microscopy of isolated cardiomyocytes from mice.

Fig. 6. Safety assessment in cynomolgus monkey.

Fig. 4. Expression of HGF in liver ECs cooperates with NOX4 inhibition to enhance engraftment of regenerative hepatocytes. Expression of HGF in liver ECs.

Fig. 3 In situ vaccination with CpG and anti-OX40 is therapeutic in a spontaneous tumor model. In situ vaccination with CpG and anti-OX40 is therapeutic.

Fig. 5 EGFR mutation status of patients with NSCLC, detected by histological examination and ARMS PCR. EGFR mutation status of patients with NSCLC, detected.

Fig. 7. KIF11 informs patient prognosis, and targeting improves survival in a preclinical model. KIF11 informs patient prognosis, and targeting improves.

Fig. 3. Recovery of AVP-deficient rats from anemia induced by sublethal irradiation. Recovery of AVP-deficient rats from anemia induced by sublethal irradiation.

Fig. 1. Neurobehavioral testing in YG8R mice transplanted with wild-type mouse HSPCs. Neurobehavioral testing in YG8R mice transplanted with wild-type.

Fig. 3. β-AR signaling induces IL-6 in NSCLC cells via activation of PKC and CREB. β-AR signaling induces IL-6 in NSCLC cells via activation of PKC and.

Fig. 5 Local gel scaffold for T cell memory response.

Fig. 4. Irisin protected against oxidative stress and apoptosis in IR-injured lung tissue. Irisin protected against oxidative stress and apoptosis in IR-injured.

Fig. 2 Fas controls IL-1RA–sEV secretion in murine MSCs.

Fig. 4. Improved tumor response to docetaxel in TNBC and trastuzumab in HER2-amplified PDX models with the addition of S Improved tumor response.

Fig. 7 Improvement of clinical score and axon pathology by nasal IL-4 treatment during chronic EAE. Improvement of clinical score and axon pathology by.

Fig. 4. Loss of DLK expression is neuroprotective in the SOD1G93A mouse model of ALS. Loss of DLK expression is neuroprotective in the SOD1G93A mouse model.

Fig. 7. Therapeutic effects of hiPSC-EPO protein on renal anemia in adenine-treated mice. Therapeutic effects of hiPSC-EPO protein on renal anemia in adenine-treated.

Fig. 8 SQLE inhibitor terbinafine suppresses NAFLD-HCC growth in vitro and in vivo. SQLE inhibitor terbinafine suppresses NAFLD-HCC growth in vitro and.

Fig. 7 CSPG4-high GBMs show more microglia than CSPG4-low GBMs and express TNFα. CSPG4-high GBMs show more microglia than CSPG4-low GBMs and express TNFα.

Fig. 5. Nutlin-3 treatment rescues the proliferation and differentiation of NPCs in vitro. Nutlin-3 treatment rescues the proliferation and differentiation.

Comparison of therapeutic efficacies of C12G6 and other bnAbs in mice

Fig. 4 TNF-α up-regulates Fas/Fap-1 expression to promote IL-1RA–sEV release in murine MSCs. TNF-α up-regulates Fas/Fap-1 expression to promote IL-1RA–sEV.

Fig. 6. pKL cells revert hyperglycemia in NOD mice in vivo.

Fig. 7. Genetic ablation of UCP2 compromised the protective effect of exogenous irisin on lung IR injury. Genetic ablation of UCP2 compromised the protective.

Fig. 7. Treatment with DLK inhibitors reduces p-c-Jun and protects against neuronal and synaptic loss in vitro and in ALS mouse models. Treatment with.

Fig. 3. VEGFR-3 signaling increases infiltration of naïve T cells in a CCR7-dependent manner. VEGFR-3 signaling increases infiltration of naïve T cells.

Bexarotene is neuroprotective in mouse and human HD neurons in vitro

Fig. 4. Acute lung injury in miR-223−/y mice.

Fig. 3 CSF1 is expressed in human melanoma.

Fig. 6 Photoreceptor cell survival in the RCS rat retina after transplantation with hESC-RPE cell sheets. Photoreceptor cell survival in the RCS rat retina.

Fig. 1 LB100 and LB102 specifically inhibit PP2A phosphatase activity and the growth of BCR-ABL+ cells. LB100 and LB102 specifically inhibit PP2A phosphatase.

Correlation of reovirus RNA/protein with proliferating tumor cells

Nox1 as a potential therapeutic target to inhibit matricellular-mediated endothelial senescence. Nox1 as a potential therapeutic target to inhibit matricellular-mediated.

Fig. 7 Transient immunosuppression (4 weeks) supports long-term graft survival and is associated with progressive decrease in spinal regional inflammatory.

Fig. 2. CD treatment facilitates regression of murine atherosclerosis.

1018-NT-β-cell clusters protect mice from STZ-induced diabetes.

Fig. 5 Treatment with BMS (PO BID) protects from wasting and colitis in two SCID mouse models. Treatment with BMS (PO BID) protects from.

Human pluripotent stem cell–derived erythropoietin-producing cells ameliorate renal anemia in mice by Hirofumi Hitomi, Tomoko Kasahara, Naoko Katagiri,

Fig. 1. The HCN channel blocker ivabradine (IVA) is analgesic in a mouse model of type 1 diabetes. The HCN channel blocker ivabradine (IVA) is analgesic.

Fig. 3. Human liver tissue seed graft function.

Candesartan diminishes SynO-mediated morphofunctional responses in microglia. Candesartan diminishes SynO-mediated morphofunctional responses in microglia.

Presentation transcript:

Fig. 8. Therapeutic effects of the transplantation of hiPSC-EPO–producing cells on renal anemia in adenine-treated mice. Therapeutic effects of the transplantation of hiPSC-EPO–producing cells on renal anemia in adenine-treated mice. Renal anemia was induced using adenine treatment (50 mg/kg body weight daily for 5 weeks) in immunodeficient mice (NOD.CB17-Prkdcscid/J mice). Twenty aggregates of hiPSC-EPO cells (5.0 × 105 cells per aggregate) were transplanted into the kidney subcapsules of mice with renal anemia. (A) Hematocrit was examined during the first 4 weeks after transplantation using glass capillary tubes. (B) Human EPO concentrations in mouse serum at 4 weeks after transplantation were measured using ELISA. (C) Hematocrit was examined for up to 28 weeks after transplantation. The gray shaded areas in (A) and (C) indicate the normal hematocrit range in NOD.CB17-Prkdcscid/J mice. (D) Human EPO concentrations in mouse serum after transplantation were measured using ELISA. (E) The hiPSC-EPO–producing cell grafts were evaluated using immunohistochemistry for EPO (green), AFP (red) and ALBUMIN (red) and using H&E staining. (F) The new vasculature in the grafts derived from host mice was examined by anti-mouse CD31/PECAM-1 immunostaining and H&E staining. (G) The human EPO concentrations in host mouse serum after phlebotomy were measured using ELISA. The data from three independent experiments are means ± SEM; n = 6 for hiPSC-EPO–producing cells and saline in (A) and (B). The data from two independent experiments are means ± SEM; n = 4 for hiPSC-EPO cells and saline in (C), (D), and (G). *P < 0.05 versus control; ANOVA with Bonferroni’s test (A, C, D, and G) and Student’s t test (B). Scale bars, 40 μm (E) and 20 μm (F). Hirofumi Hitomi et al., Sci Transl Med 2017;9:eaaj2300 Published by AAAS