An in vitro cell culture system to study the influence of external pneumatic compression on endothelial function  Guohao Dai, MSa, Olga Tsukurov, MDb,

Slides:



Advertisements
Similar presentations
Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,
Advertisements

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.
Date of download: 10/2/2017 Copyright © ASME. All rights reserved.
The effect of cigarette smoke, nicotine, and carbon monoxide on the permeability of the arterial wall  D.R. Allen, F.R.C.S., N.L. Browse, M.D., F.R.C.S.,
Shear stress magnitude and directionality modulate growth factor gene expression in preconditioned vascular endothelial cells  Anthony G. Passerini, PhDa,
The influence of early-phase remodeling events on the biomechanical properties of engineered vascular tissues  Zehra Tosun, Carolina Villegas-Montoya,
The effect of cigarette smoke, nicotine, and carbon monoxide on the permeability of the arterial wall  D.R. Allen, F.R.C.S., N.L. Browse, M.D., F.R.C.S.,
Richard T. Purdy, M. D. , Frederick C. Beyer, M. D. , William D
A compliant tubular device to study the influences of wall strain and fluid shear stress on cells of the vascular wall  Aziz Benbrahim, MD, Gilbert J.
A new vascular Endostaple: A technical description
Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells  Paul DiMuzio, MD, Thomas Tulenko, PhD  Journal.
Improving walking ability and ankle brachial pressure indices in symptomatic peripheral vascular disease with intermittent pneumatic foot compression:
Endovenous treatment of the greater saphenous vein with a 940-nm diode laser: Thrombotic occlusion after endoluminal thermal damage by laser-generated.
The percutaneous Greenfield filter: Outcomes and practice patterns
Three-dimensional flow characteristics in ventricular assist devices: Impact of valve design and operating conditions  Christoph Benk, MSc, Ramona Lorenz,
A new valvulotome for in situ bypass grafts
Louis Allard, MSc, Guy Cloutier, PhD  Journal of Vascular Surgery 
Ambulatory venous pressure revisited
Michael A. Golden, MD, Y. P. Tina Au, PhD, Richard D
Effect of retroviral transduction on human endothelial cell phenotype and adhesion to Dacron vascular grafts  Ronald J. Jankowski, MS, Donald A. Severyn,
Malcolm O. Perry, MD, Richard Kempczinski, MD 
Recurrent thoracic outlet syndrome
Alterations in aortic endothelial cell morphology and cytoskeletal protein synthesis during cyclic tensional deformation  Bauer E. Sumpio, M.D., Ph.D.,
Protecting the brain and spinal cord
Prediction of rupture risk in abdominal aortic aneurysm during observation: Wall stress versus diameter  Mark F. Fillinger, MDa, Steven P. Marra, PhDa,b,
The fate of an endothelium layer after preconditioning
Mark H. Meissner, MDa, Brenda K. Zierler, RN, PhDa, Robert O
A systematic review of pneumatic compression for treatment of chronic venous insufficiency and venous ulcers  Elise Berliner, PhD, Berrin Ozbilgin, MBA,
Michael L. Klyachkin, MD, Sandip Dhara, BS, Gilbert J
Gabriele Di Luozzo, MD, Jaya Bhargava, PhD, Richard J. Powell, MD 
Comparison of abdominal aortic hemodynamics between men and women at rest and during lower limb exercise  Christopher P. Cheng, MSa, Robert J. Herfkens,
A prospective randomized trial comparing vein with polytetrafluoroethylene in above- knee femoropopliteal bypass grafting  Desiree H.C. Burger, MDa, A.Pieter.
Hwa-Liang Leo, MSa, Zhaoming He, PhDb, Jeffrey T. Ellis, PhDa, Ajit P
Stanley E. Rittgers, PhD, Mark C.S. Shu, PhD 
Luminal surface concentration of lipoprotein (LDL) and its effect on the wall uptake of cholesterol by canine carotid arteries  Xiaoyan Deng, PhD, Yves.
Ambient oxygen tension modulates endothelial fibrinolysis
Matadial Ojha, PhD, Richard S. C. Cobbold, PhD, K
M. Ojha, PhD, K. W. Johnston, MD, FRCS(C), R. S. C. Cobbold, PhD, R. L
Limited B-mode venous imaging versus complete color-flow duplex venous scanning for detection of proximal deep venous thrombosis  Roberta Poppiti, BS,
Objective tinnitus resulting from internal carotid artery stenosis
Nicotine and cotinine stimulate secretion of basic fibroblast growth factor and affect expression of matrix metalloproteinases in cultured human smooth.
G. W. H. Schurink, MD, J. M. van Baalen, MD, M. J. T. Visser, MD, J. H
Intracellular calcium response of endothelial cells exposed to flow in the presence of thrombin or histamine  Laura M. Worthen, MS, Matthias U. Nollert,
The Journal of Vascular Surgery: 1982 to 1990
The effect of carbon coating and porosity on early patency of expanded polytetrafluoroethylene grafts: An experimental study  Donald L. Akers, MD, Yong.
Reviewer Acknowledgment
Endothelial cell harvest for seeding vascular prostheses: The influence of technique on cell function, viability, and number  Bernard M. Bourke, M.B.B.S.,
Atul K. Madan, MDa, Thomas A. Santora, MDb, Verdi J. DiSesa, MDc 
Gregory G. Couch, MASc, K.Wayne Johnston, MD, FRCS, Matadial Ojha, PhD 
Calvin B. Ernst, MD  Journal of Vascular Surgery 
Cellular repopulation of human vein allograft bypass grafts
Cardiovascular surgery—The rocket and its stars: Presidential address
Presidential address: A brief history of arterial blood flow—from Harvey and Newton to computational analysis  Joseph P. Archie, PhD, MD  Journal of Vascular.
Thorascopic dorsal sympathectomy for hyperhidrosis: A new approach
John O.F. Roehm, M.D.  Journal of Vascular Surgery 
Ambulatory venous pressure revisited
Prophylactic vena caval filters in trauma: The rest of the story
J.Dennis Baker, MD  Journal of Vascular Surgery 
Nitroglycerin to control blood pressure during endovascular stent-grafting of descending thoracic aortic aneurysms  Emanuel O. Bernard, MD, Edith R. Schmid,
Outcomes after surgery for thoracic outlet syndrome
Biochemical analysis of a human humoral fibroblast inhibitory factor associated with impaired vascular prosthetic graft incorporation  Jacob Schneiderman,
Exposure of femoral vessels by a suprainguinal incision
D. Emerick Szilagyi, MD—An appreciation
Presidential address: The second-generation vascular surgeon
Radiologic investigation of abdominal aortic aneurysm disease: Comparison of three modalities in staging and the detection of inflammatory change  William.
George D. Lilly 1906–1988 Journal of Vascular Surgery
James A. DeWeese, MD  Journal of Vascular Surgery 
Jonathan Golledge, FRCS, Rachel Cuming, BSc, David K
Innominate artery trauma: A thirty-year experience
J.Jeffrey Alexander, MD, Remedios Miguel, MS, Joseph J. Piotrowski, MD 
Presentation transcript:

An in vitro cell culture system to study the influence of external pneumatic compression on endothelial function  Guohao Dai, MSa, Olga Tsukurov, MDb, Roslyn W. Orkin, PhDb, William M. Abbott, MDb, Roger D. Kamm, PhDa, Jonathan P. Gertler, MDb  Journal of Vascular Surgery  Volume 32, Issue 5, Pages 977-987 (November 2000) DOI: 10.1067/mva.2000.110357 Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions

Fig. 1 A , Schematic drawing of VFS. Four totally independent flow circuits are shown, corresponding to the four cases in Table I. The air pump simultaneously drives the pulsed flow and the bellows to produce collapse. The reservoirs upstream of the test chambers are maintained at the proper carbon dioxide partial pressures. B, Test chamber used to produce tube compression. Air inflates the bellows, driving down the pusher plate to a position determined by small pegs inserted through bottom to desired level. Inlet and outlet are designed to minimize flow disturbance. Journal of Vascular Surgery 2000 32, 977-987DOI: (10.1067/mva.2000.110357) Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions

Fig. 2 Measured pressure (top ) flow rate (middle ), and calculated shear stress (bottom ) in the VFS for condition F. Measurements are made at the locations indicated in Fig 1, A. Shear stress is calculated with the Womersley theory,20 which is based on measured time-varying flow rate. Journal of Vascular Surgery 2000 32, 977-987DOI: (10.1067/mva.2000.110357) Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 Color contour map showing calculated tube wall strain computed with finite element code ABAQUS and properties of silicon rubber. Note that maximal principal strains range between 0% and 10%. ECs cultured in the inner layer of tube are subjected to same level of deformation. Note that at upper and lower walls of tube, cells are stretched by about 10%. Journal of Vascular Surgery 2000 32, 977-987DOI: (10.1067/mva.2000.110357) Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions

Fig. 4 Endothelial morphology after 6 hours of experiment. Flow direction is left to right in each photograph. Alignment is strongest in FC and F and cannot be detected in C or Cntrl (magnification = 200×). Cntrl, Control. Journal of Vascular Surgery 2000 32, 977-987DOI: (10.1067/mva.2000.110357) Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions

Fig. 5 Northern Blot of mRNA expression of tPA, eNOS, and GAPD (top to bottom ). Lane 1-4 (left to right ) represents FC, C, F and Control group, respectively. Journal of Vascular Surgery 2000 32, 977-987DOI: (10.1067/mva.2000.110357) Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions