Human Cord Blood Stem Cells Enhance Neonatal Right Ventricular Function in an Ovine Model of Right Ventricular Training Ben Davies, MRCS(Eng), PhD, Ngaire.

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Presentation transcript:

Human Cord Blood Stem Cells Enhance Neonatal Right Ventricular Function in an Ovine Model of Right Ventricular Training Ben Davies, MRCS(Eng), PhD, Ngaire J. Elwood, PhD, Shan Li, MS, Fiona Cullinane, FRANZCOG, Glenn A. Edwards, BVSc, MACVSs, Donald F. Newgreen, PhD, Christian P. Brizard, MD The Annals of Thoracic Surgery Volume 89, Issue 2, Pages 585-593.e4 (February 2010) DOI: 10.1016/j.athoracsur.2009.10.035 Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Screening of sheep tissues 1 month after transplantation, by DNA polymerase chain reaction using probes specific for human β-2 microglobulin sequences. β-Actin lanes demonstrate presence of amplifiable DNA. Representative gel from a sheep transplanted at 10 days of age followed by tissue collection 30 days after transplantation. (BM = bone marrow; Kid = kidney; Liv = liver; Lu = lung; LV = left ventricle; RV = right ventricle; Spl = spleen.) The Annals of Thoracic Surgery 2010 89, 585-593.e4DOI: (10.1016/j.athoracsur.2009.10.035) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Immunohistochemistry with antibody against human nuclear antigen 1 month after transplantation (transplanted at 1 week of life) in the right ventricle (A), kidney (B), spleen (C), and bone marrow (D). (Human cells indicated by arrowheads.) The Annals of Thoracic Surgery 2010 89, 585-593.e4DOI: (10.1016/j.athoracsur.2009.10.035) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Immunohistochemistry for human-specific Alu gene 1 month after transplantation n the right ventricle (A), kidney (B), spleen (C), and bone marrow (D). The arrowheads show human nuclei identified by in situ hybridization for human Alu sequences. (Scale bars indicate 50 μm.) The Annals of Thoracic Surgery 2010 89, 585-593.e4DOI: (10.1016/j.athoracsur.2009.10.035) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 Confocal microscopy of ovine tissues 1 month after transplantation in the right ventricle showing positivity for human nuclear antigen (HNA) and CD45 (A), the kidney showing CD45-positive human cell (B), the spleen showing cluster of CD45-positive human cells (C), the spleen showing positivity for human CD23 (D), the bone marrow showing positivity for HNA and CD45 (E), and the bone marrow showing positivity for human CD23 (F). (DAPI = 4′-6-Diamidino-2-phenylindole.) (All scale bars indicate 20 μm.) The Annals of Thoracic Surgery 2010 89, 585-593.e4DOI: (10.1016/j.athoracsur.2009.10.035) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Appendix Fig 1 Trial design. The Annals of Thoracic Surgery 2010 89, 585-593.e4DOI: (10.1016/j.athoracsur.2009.10.035) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Appendix Fig 2 Diagram of operative setup. The Annals of Thoracic Surgery 2010 89, 585-593.e4DOI: (10.1016/j.athoracsur.2009.10.035) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions