A Ligand of Human Thy-1 is Localized on Polymorphonuclear Leukocytes and Monocytes and Mediates the Binding to Activated Thy-1-Positive Microvascular.

Slides:

Advertisements

Similar presentations

The CXC Receptor 2 Is Overexpressed in Psoriatic Epidermis

Advertisements

Autoantibodies in Scurfy Mice and IPEX Patients Recognize Keratin 14

Importance of Tissue Transglutaminase in Repair of Extracellular Matrices and Cell Death of Dermal Fibroblasts After Exposure to a Solarium Ultraviolet.

Keloid Fibroblasts Resist Ceramide-Induced Apoptosis by Overexpression of Insulin- Like Growth Factor I Receptor Hiroshi Ishihara, Hiroshi Yoshimoto,

Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes Genji Imokawa, Yutaka Takagi,

Expression and Role of IL-15 in Post-Burn Hypertrophic Scars

Peroxiredoxin is Ubiquitously Expressed in Rat Skin: Isotype-Specific Expression in the Epidermis and Hair Follicle Jeong Eun Lee, Byung Dae Kwon, Jee-Bum.

BM-40(Osteonectin, SPARC) Is Expressed Both in the Epidermal and in the Dermal Compartment of Adult Human Skin Nicholas Hunzelmann, Martin Hafner, Sabine.

Cord Blood CD34+ Cells Differentiate into Dermal Dendritic Cells in Co-Culture with Cutaneous Fibroblasts or Stromal Cells Zia U.A. Mollah, Setsuya Aiba,

Establishment of Rat Dermal Papilla Cell Lines that Sustain the Potency to Induce Hair Follicles from Afollicular Skin Mutsumi Inamatsu, Takashi Matsuzaki,

IC3b Arrests Monocytic Cell Differentiation Into CD1c-Expressing Dendritic Cell Precursors: A Mechanism for Transiently Decreased Dendritic Cells in vivo.

Activation-Dependent Modulation of Hyaluronate-Receptor Expression and of Hyaluronate-Avidity by Human Monocytes Johannes M. Weiss, Andreas C. Renkl,

Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes Marcel B.M. Teunissen, Jan.

Human Keratinocytes Express Functional CD14 and Toll-Like Receptor 4

Interaction of HSV-1 Infected Peripheral Blood Mononuclear Cells with Cultured Dermal Microvascular Endothelial Cells: a Potential Model for the Pathogenesis.

Resistance to Activation-Induced Cell Death and Bystander Cytotoxicity Via the Fas/Fas Ligand Pathway Are Implicated in the Pathogenesis of Cutaneous.

Role of Monocyte Chemoattractant Protein-1 and its Receptor,CCR-2, in the Pathogenesis of Bleomycin-Induced Scleroderma Toshiyuki Yamamoto, Kiyoshi Nishioka

Maria A. Vidal, Angel Astroza, Carola E

Ahmad Waseem, Yasmin Alam, Anand Lalli

Transient CD44 Variant Isoform Expression and Reduction in CD4+/CD25+ Regulatory T Cells in C3H/HeJ Mice with Alopecia Areata Margot Zöller, Kevin J.

Manuela Schmidt, Danny Gutknecht, Jan C

Thy-1/β3 Integrin Interaction-Induced Apoptosis of Dermal Fibroblasts Is Mediated by Up-Regulation of FasL Expression Manuela Schmidt, Danny Gutknecht,

Hyaluronan-Independent Adhesion of CD44H+ and CD44v10+ Lymphocytes to Dermal Microvascular Endothelial Cells and Keratinocytes Tatjana K. Weimann, Christine.

Activation of Tissue Inhibitor of Metalloproteinases-3 (TIMP-3) mRNA Expression in Scleroderma Skin Fibroblasts Laura Mattila, Kristiina Airola, Matti.

Membrane Type 1 Matrix Metalloproteinase Regulates Cellular Invasiveness and Survival in Cutaneous Epidermal Cells Usha Nagavarapu, Kenneth Relloma,

Mitigation of Delayed-Type Hypersensitivity Reactions by a CD44 Variant Isoform v3- Specific Antibody: Blockade of Leukocyte Egress Simone Seiter, Peter.

IGF-II-Mediated COX-2 Gene Expression in Human Keratinocytes Through Extracellular Signal-Regulated Kinase Pathway Hye Jung Kim, Tae-Yoon Kim Journal.

An Autocrine Loop Mediates Expression of Vascular Endothelial Growth Factor in Human Dermal Microvascular Endothelial Cells Barbara Vega-Diaz, Serge.

Early- and Late-Stage Kaposi's Sarcoma-Derived Cells But Not Activated Endothelial Cells Can Invade De-Epidermized Dermis Thierry Simonart, Chantal Degraef,

Heparin-Binding Epidermal-Growth-Factor-Like Growth Factor Activation of Keratinocyte ErbB Receptors Mediates Epidermal Hyperplasia, a Prominent Side-Effect.

Katrin Pauls, Margarete Schön, Robert C

A Mannose-Binding Receptor is Expressed on Human Keratinocytes and Mediates Killing of Candida albicans Gyözö Szolnoky, Zsuzsanna Bata-Csörgö, Anna Sz.

Increased Neutrophil Adherence in Psoriasis: Role of the Human Endothelial Cell Receptor Thy-1 (CD90) Anne Wetzel, Tino Wetzig, Uwe F. Haustein, Michael.

Toshiyuki Yamamoto, Kiyoshi Nishioka

Decrease in Epidermal CD44 Expression as a Potential Mechanism for Abnormal Hyaluronate Accumulation in Superficial Dermis in Lichen Sclerosus et Atrophicus

Pancreatitis Associated Protein I (PAP-I) Alters Adhesion and Motility of Human Melanocytes and Melanoma Cells Christine Valery, Jean-Jacques Grob, Patrick.

High Invasive Melanoma Cells Induce Matrix Metalloproteinase-1 Synthesis in Fibroblasts by Interleukin-1α and Basic Fibroblast Growth Factor-Mediated.

Characterization of the CC Chemokine Receptor 3 on Human Keratinocytes

Rolipram Inhibits Polarization and Migration of Human T Lymphocytes

Ekatherina Vassina, Martin Leverkus, Shida Yousefi, Lasse R

Interleukin-18 and the Costimulatory Molecule B7-1 Have a Synergistic Anti-Tumor Effect on Murine Melanoma; Implication of Combined Immunotherapy for.

Increased Oxidative Damage to Fibroblasts in Skin With and Without Lesions in Psoriasis Stéphanie Dimon-Gadal, Pascale Gerbaud, Jean Guibourdenche, Danièle.

CD4+ CD56+ Blastic Tumor Cells Express CD101 Molecules

Human Dendritic Cells as Targets of Dengue Virus Infection

Arsenic Induces Tumor Necrosis Factor α Release and Tumor Necrosis Factor Receptor 1 Signaling in T Helper Cell Apoptosis Hsin-Su Yu, Gwo-Shing Chen

Basal-Cell Adhesion Molecule (B-CAM) is Induced in Epithelial Skin Tumors and Inflammatory Epidermis, and is Expressed at Cell–Cell and Cell–Substrate.

Involvement of αvβ5 Integrin in the Establishment of Autocrine TGF-β Signaling in Dermal Fibroblasts Derived from Localized Scleroderma Yoshihide Asano,

Increased Expression of Endothelial Cell Adhesion Molecules Due to Mediator Release from Human Foreskin Mast Cells Stimulated by Autoantibodies in Chronic.

Human CD4+ T Lymphocytes with Remarkable Regulatory Functions on Dendritic Cells and Nickel-Specific Th1 Immune Responses Andrea Cavani, Francesca Nasorri,

Intranuclear Localization of Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) During Cell Division in Human Keratinocytes Christopher J. Wraight,

Scleroderma Fibroblasts Demonstrate Enhanced Activation of Akt (Protein Kinase B) In Situ Jae-Bum Jun, Melanie Kuechle, Junki Min, Seung Cheol Shim,

Αvβ6 Integrin Upregulates Matrix Metalloproteinase 9 and Promotes Migration of Normal Oral Keratinocytes Gareth J. Thomas, S. Poomsawat, Mark P. Lewis,

Selective Upregulation of Fibroblast Fas Ligand Expression, and Prolongation of Fas/Fas Ligand-Mediated Skin Allograft Survival, by Retinoic Acid: the.

James Gailit, Mary J. Marchese, Richard R. Kew, Barry L. Gruber

Interferon-γ, a Strong Suppressor of Cell Proliferation, Induces Upregulation of Keratin K6, One of the Inflammatory- and Proliferation-Associated Keratins

Β2AR Antagonists and β2AR Gene Deletion Both Promote Skin Wound Repair Processes Christine E. Pullar, Gabrielle S. Le Provost, Andrew P. O'Leary, Sian.

1α,25-Dihydroxyvitamin D3 Stimulates Activator Protein 1 DNA-Binding Activity by a Phosphatidylinositol 3-Kinase/Ras/MEK/Extracellular Signal Regulated.

Hidetoshi Takahashi, Akemi Ishida-Yamamoto, Hajime Iizuka

Soluble FAS Ligand: A Discriminating Feature between Drug-Induced Skin Eruptions and Viral Exanthemas Karoline Stur, Franz M. Karlhofer, Georg Stingl

Melanoma Cells Control HA Synthesis in Peritumoral Fibroblasts via PDGF-AA and PDGF-CC: Impact on Melanoma Cell Proliferation Anja Willenberg, Anja Saalbach,

Tomoyuki Ohtani, Setsuya Aiba, Masato Mizuashi, Zia U. A

Ligation of the β4 Integrin Triggers Adhesion Behavior of Human Keratinocytes by an “Inside-out” Mechanism Stefan Kippenberger, Stefan Loitsch, Jutta.

Regulation of Tissue Factor in Microvascular Dermal Endothelial Cells

Aberrant Expression of Adhesion Molecules by Sézary Cells: Functional Consequences Under Physiologic Shear Stress Conditions Sam T. Hwang, David J. Fitzhugh

Human Leukocyte Elastase Induces Keratinocyte Proliferation by Epidermal Growth Factor Receptor Activation Ulf Meyer-Hoffert, Jana Wingertszahn, Oliver.

Inhibition of IgE-Production by Peroxisome Proliferator-Activated Receptor Ligands Ralph Rühl, Anja Dahten, Florian J. Schweigert, Udo Herz, Margitta.

No Evidence of Human Herpesvirus 8 Infection in Patients with Paraneoplastic Pemphigus, Pemphigus Vulgaris, or Pemphigus Foliaceus Sandra S. Cohen, Andrew.

Induction of Interferon Regulatory Factor 1 Expression in Human Dermal Endothelial Cells by Interferon-γ and Tumor Necrosis Factor-α Is Transcriptionally.

Errata Journal of Investigative Dermatology

Matrix Metalloproteinase Inhibitor BB-3103 Unlike the Serine Proteinase Inhibitor Aprotinin Abrogates Epidermal Healing of Human Skin Wounds Ex Vivo1

Presentation transcript:

A Ligand of Human Thy-1 is Localized on Polymorphonuclear Leukocytes and Monocytes and Mediates the Binding to Activated Thy-1-Positive Microvascular Endothelial Cells and Fibroblasts Anja Saalbach, Uwe F. Haustein, Ulf Anderegg Journal of Investigative Dermatology Volume 115, Issue 5, Pages 882-888 (November 2000) DOI: 10.1046/j.1523-1747.2000.00104.x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Thy-1 ligand is expressed on inflammatory cells in situ. Cryostat sections of bullous pemphigoid were incubated with biotinylated bovine serum albumin (a) as negative control, MoAb AS02 (b, c) or purified and biotinylated human Thy-1 (d–f). The biotinylated albumin showed no reaction (a). The Thy-1 antigen detected by MoAb AS02 was expressed on EC and fibroblasts (b, c). The Thy-1 ligand was detected on inflammatory cells (red) using the biotinylated Thy-1. Keratinocytes, fibroblasts, and EC did not express the Thy-1 ligand. Scale bar: 25 μm. Journal of Investigative Dermatology 2000 115, 882-888DOI: (10.1046/j.1523-1747.2000.00104.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Thy-1 ligand is expressed on PNC and monocytes detected by flow cytometry. PNC, MNC, and monocytes were labeled with biotinylated Thy-1 and bovine serum albumin as negative control followed by streptavidin–allophycocyanin and analyzed by flow cytometry. PNC and monocytes could bind the biotinylated Thy-1 whereas lymphocytes did not. Biotinylated albumin did not react with any of these cell types. Preincubation of PNC with unlabeled Thy-1 was able to chase the signal of biotinylated Thy-1-binding. Journal of Investigative Dermatology 2000 115, 882-888DOI: (10.1046/j.1523-1747.2000.00104.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Establishment of experimental cell models consisting of Thy-1-positive and Thy-1-negative HDMEC or fibroblasts. Phorbol 12-myristate 13-acetate-stimulated HDMEC, a fibroblast cell line (A2a), and subsequently Thy-1-positive and Thy-1-negative cell populations isolated by immunomagnetic separation using the MoAb AS02 as described in Materials and Methods were tested for the expression of Thy-1 by flow cytometry using MoAb AS02 and goat anti-mouse fluorescein isothiocyanate. A mouse isotype antibody was used as a control. Journal of Investigative Dermatology 2000 115, 882-888DOI: (10.1046/j.1523-1747.2000.00104.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Adhesion of PNC to Thy-1-positive HDMEC is increased compared with Thy-1-negative HDMEC and is inhibited by blocking Thy-1. Confluent Thy-1-positive and Thy-1-negative HDMEC were incubated with biotinylated PNC, unbound cells were removed, and attached cells were visualized by streptavidin–CY3. Ten microscopic fields (magnification 200×) were counted. Thy-1-positive HDMEC bound about two times more PNC than Thy-1-negative HDMEC. Blocking of Thy-1 inhibited the adhesion of PNC to Thy-1-positive HDMEC up to about 50% whereas the binding of PNC to Thy-1-negative HDMEC was not effected. Preincubation of PNC with purified Thy-1 (1 μg per ml, 15 min, 4°C) also effectively inhibited cell adhesion to Thy-1-positive HDMEC. Results represent the mean ± SD of five independent experiments. Journal of Investigative Dermatology 2000 115, 882-888DOI: (10.1046/j.1523-1747.2000.00104.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Adhesion of monocytes to Thy-1-positive HDMEC is increased compared with Thy-1-negative HDMEC, and is inhibited by blocking Thy-1. Confluent Thy-1-positive and Thy-1-negative HDMEC were incubated either with biotinylated monocytes, unbound cells were removed and attached cells were visualized by streptavidin–CY3. Ten microscopic fields (magnification 200×) were counted. Thy-1-positive HDMEC bound about 2.5 times more monocytes than Thy-1-negative HDMEC. Preincubation of HDMEC with MoAb AS01/AS02 cross-linked by a goat anti-mouse F(ab)2 antibody lead to an decreased number of attached monocytes to Thy-1-positive HDMEC, whereas the adhesion to Thy-1-negative HDMEC was not changed by the antibodies. Preincubation of monocytes with purified Thy-1 (1 μg per ml, 15 min, 4°C) also effectively inhibited cell adhesion to Thy-1-positive HDMEC. Results represent the mean ± SD of five independent experiments. Journal of Investigative Dermatology 2000 115, 882-888DOI: (10.1046/j.1523-1747.2000.00104.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Adhesion of PNC to Thy-1-positive fibroblasts is increased compared with Thy-1-negative fibroblasts. Confluent Thy-1-positive and Thy-1-negative immortalized fibroblasts (A2a) were incubated with biotinylated PNC or monocytes, unbound cells were removed and attached cells were visualized by streptavidin–CY3 and counting 10 microscopic fields (magnification 200 ×). Thy-1-positive fibroblasts bound two times more PNC than Thy-1-negative fibroblasts. Adhesion of monocytes to Thy-1-positive and Thy-1-negative fibroblasts did not differ. Data represent the mean ± SD of seven independent experiments. Journal of Investigative Dermatology 2000 115, 882-888DOI: (10.1046/j.1523-1747.2000.00104.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 Blocking of Thy-1 decreased the adhesion of PNC and partially of monocytes to primary fibroblasts. Confluent human dermal primary fibroblasts were incubated either with MoAb AS01/AS02 cross-linked by a goat anti-mouse F(ab)2 antibody, with an anti-HLA-ABC antibody as control antibody or without antibody. Then, cells were incubated with biotinylated PNC or monocytes, unbound cells were removed and attached cells were visualized by streptavidin–CY3 and counting 10 microscopic fields (magnification 200×). Blocking of Thy-1 inhibited the adhesion of PNC to fibroblasts up to 79% and the adhesion of monocytes up to 20%. The anti-HLA-ABC antibody did not decrease the adherence of PNC to fibroblasts compared with the control without antibody incubation. Further, the adhesion of PNC to fibroblasts could be blocked very efficiently by preincubation of PNC with purified Thy-1. Data represent the mean ± SD of five independent experiments. Journal of Investigative Dermatology 2000 115, 882-888DOI: (10.1046/j.1523-1747.2000.00104.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions