Alterations in wall tension and shear stress modulate tyrosine kinase signaling and wall remodeling in experimental vein grafts Tam T.T. Huynh, MD, Mark.

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Presentation transcript:

Alterations in wall tension and shear stress modulate tyrosine kinase signaling and wall remodeling in experimental vein grafts Tam T.T. Huynh, MD, Mark G. Davies, MD, PhD, Matthew J. Trovato, Einar Svendsen, MD, PhD, Per-Otto Hagen, PhD Journal of Vascular Surgery Volume 29, Issue 2, Pages 334-344 (February 1999) DOI: 10.1016/S0741-5214(99)70386-1 Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 A, Representative Western blot test of phosphorylated tyrosine residues in control vein grafts (A and C) and in tube-supported vein grafts (B and D). Molecular weight markers (in kDa) are shown at left. Three days after bypass graft surgery, phosphorylated tyrosine residues were detected with PY-20 antibody (1 mg/mL; Chemicon International, Inc, Temecula, Calif) in control vein grafts in proteins at just above 82, 113, and 200 markers. Only proteins with approximately 113 kDa molecular weight were tyrosine phosphorylated in tube-supported vein grafts. Samples were run in duplicate, and similar results were found in other Western blot tests. B, Densitometric analysis of protein bands (integrated density in relative units) showed 15-fold reduction in tyrosine kinase activity with tube support as compared with grafts without tube support (control) by means of unpaired Student t test (P < .001). Values are mean ± standard error of mean (n = 4 per group). Journal of Vascular Surgery 1999 29, 334-344DOI: (10.1016/S0741-5214(99)70386-1) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 A, Representative composite photomicrograph of cross-sections from tube-supported vein graft (A) and control vein graft (B). At 28 days, intimal thickness was decreased by 45% in tube-supported vein grafts as compared with controls (46 ± 2 μm vs 84 ± 5 μm, respectively; P < .0001). Thickness of the media also was decreased, although to a lesser extent (by 20%) with tube support as compared with no tube support (63 ± 8 μm vs 79 ± 4 μm, respectively; P < .05). Top is the lumen. Arrowheads delineate demarcation between intima and media. Original magnification, ×250. H, Intimal hyperplasia; M, media; *, collagen tube. B, Schematic diagram shows thickness of media (M) and intima (I) in relation to luminal diameter (D) in tube-supported vein graft as compared with control vein graft. Luminal index = luminal diameter ÷ (intimal + medial thicknesses). Note smaller diameter and lesser intimal and medial thicknesses in tube-supported vein graft as compared with control vein graft (not to scale). Journal of Vascular Surgery 1999 29, 334-344DOI: (10.1016/S0741-5214(99)70386-1) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Scanning electron micrograph of representative tube-supported vein graft at day 28 (A) and control vein graft (B). Endothelial lining is confluent and cell borders are well defined (arrowheads) in both vein grafts. Note unaltered and flattened shape of endothelial cells in tube-supported vein graft. In contrast, endothelial cells are cuboidal and slightly bulging in control vein graft. Arrows point to stomata. Original magnification, ×1250. Journal of Vascular Surgery 1999 29, 334-344DOI: (10.1016/S0741-5214(99)70386-1) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Transmission electron micrograph of representative cross-section of tube-supported vein graft at day 28 (A) and control vein graft (B). Endothelial cells (EC) are well defined in both vein grafts. Intimal smooth muscle cells (SMC) are elongated and organized in several layers in tube-supported vein graft as compared with more rounded-like and disorganized smooth muscle cells in control vein graft. Also, there is more subendothelial edema (*) in control graft than in tube-supported vein graft. Original magnification, ×4500. L, Lumen. Journal of Vascular Surgery 1999 29, 334-344DOI: (10.1016/S0741-5214(99)70386-1) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Maximal contractile responses of tube-supported (solid bar) and control (open bar) vein grafts to norepinephrine, serotonin, and bradykinin. Both groups of vein grafts generated similar contractile forces (tension) in response to all three agonists. Contractile ratio = actual maximal contraction to agonist (in mg) ÷ maximal contraction to 60 mmol/L KCl (in mg). Values are mean ± standard error of mean (n = 5 per group). Unpaired Student t test was used to compare results of two groups. Journal of Vascular Surgery 1999 29, 334-344DOI: (10.1016/S0741-5214(99)70386-1) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 6 Dose-response curves for tube-supported (solid bar) and control (open bar) vein grafts to acetylcholine (A) and nitroprusside (B). Control vein grafts had no response to acetylcholine (10–9 to 10–4 mol/L). Tube-supported vein grafts, however, showed dose-dependent relaxation to acetylcholine with maximal relaxation of 64% of precontracted tension. Both tube-supported and control vein grafts responded to nitroprusside in dose-dependent manner with maximal relaxation of greater than 100% of precontracted tension. Values are mean ± standard error of mean (n = 5 per group). Journal of Vascular Surgery 1999 29, 334-344DOI: (10.1016/S0741-5214(99)70386-1) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions