Cell-Type-Specific Optogenetics in Monkeys

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Cell-Type-Specific Optogenetics in Monkeys Vijay Mohan K. Namboodiri, Garret D. Stuber Cell Volume 166, Issue 6, Pages 1366-1368 (September 2016) DOI: 10.1016/j.cell.2016.08.047 Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 1 Dual Virus-Based Cell-Type-Specific Expression of ChR2 A virus cocktail containing two viruses with the TH promoter fragment driving Cre recombinase and a Cre-inducible ChR2 (using the double inverted open reading frame provided by loxP and lox2272 sites) is injected into the region of interest (VTA), which contains many different cell types. Cre is expressed only in cells that endogenously express TH due to the presence of the TH promoter fragment. Thus, Cre-dependent recombination will only be present in TH+ cells, therefore resulting in ChR2-eYFP expression only in these cells. Electrophysiological recordings from the population can then be used in combination with optical tagging to attain cell-type specificity. Cell 2016 166, 1366-1368DOI: (10.1016/j.cell.2016.08.047) Copyright © 2016 Elsevier Inc. Terms and Conditions