Sequencing at 10,000x using Illumina paired reads

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Sequencing at 10,000x using Illumina paired reads 9 mut. alleles in 10,000 3 mut. alleles in 10,000 Without error correction : 65 mut. alleles in 10,000 S. Marsilio et al. Supplementary Figure 3A

Sequencing at 100,000x doubles the sensitivity 4 mut. alleles in 10,000 1-2 mut. alleles in 10,000 S. Marsilio et al. Supplementary Figure 3B

1,000,000x does not significantly improve sensitivity 3 mut. alleles in 10,000 1 mut. alleles in 10,000 S. Marsilio et al. Supplementary Figure 3C

Sensitivity of detecting transitions versus transversions >5 transition mutant alleles in 10,000 4 mutant alleles in 10,000 >1 transversion mutant alleles in 10,000 S. Marsilio et al. Supplementary Figure 3D