Colocalisation of NLRP3 specks with cleaved interleukin (IL)-1β in bronchoalveolar lavage (BAL) macrophages, and nontypeable Haemophilus influenzae (NTHi)-stimulated.

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Colocalisation of NLRP3 specks with cleaved interleukin (IL)-1β in bronchoalveolar lavage (BAL) macrophages, and nontypeable Haemophilus influenzae (NTHi)-stimulated monocyte-derived macrophages. Colocalisation of NLRP3 specks with cleaved interleukin (IL)-1β in bronchoalveolar lavage (BAL) macrophages, and nontypeable Haemophilus influenzae (NTHi)-stimulated monocyte-derived macrophages. BAL macrophages cultured for 24 h in a) control medium or b) in presence of NTHi both revealed specks of NLRP3 (red) and cleaved IL-1β (green) in colocalisation (yellow/orange; arrows). Monocyte-derived macrophages showed c) only homogenous fluorescence when cultured in control medium, but d) revealed numerous NLRP3/cleaved IL-1β specks (arrows) when stimulated with NTHi. Blue is the pseudocolour of DAPI (4',6-diamidino-2-phenylindole). Scale bars=20 μm. Images are representative confocal microphotos of cells from at least four donors. Alice C-H. Chen et al. ERJ Open Res 2018;4:00130-2017 ©2018 by European Respiratory Society