Susan C. Foley, MD, David Préfontaine, MSc, Qutayba Hamid, MD, PhD

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Presentation transcript:

Images in allergy and immunology: Role of eosinophils in airway remodeling Susan C. Foley, MD, David Préfontaine, MSc, Qutayba Hamid, MD, PhD Journal of Allergy and Clinical Immunology Volume 119, Issue 6, Pages 1563-1566 (June 2007) DOI: 10.1016/j.jaci.2007.03.040 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Immunostaining for EG2-positive cells in bronchial biopsies from subjects with mild (A) and severe (B) asthma. Reaction was developed with Fast red. Positive staining for EG2 in the nasal epithelium and submucosa of a subject with allergic rhinitis (C) compared with the absence of immunoreactive cells in a control subject (D). EG2 immunoreactivity was developed with diaminobenzidine. Magnification ×200. Journal of Allergy and Clinical Immunology 2007 119, 1563-1566DOI: (10.1016/j.jaci.2007.03.040) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 TGF-β1 immunoreactive protein in a bronchial biopsy of a patient with moderate asthma (A) and double immunostaining showing the presence of TGF- β1 immunoreactivity in EG2-positive eosinophils (B). Journal of Allergy and Clinical Immunology 2007 119, 1563-1566DOI: (10.1016/j.jaci.2007.03.040) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 In situ hybridization with digoxigenin for IL-11 mRNA (dark brown/purple staining) in epithelium and subepithelium of bronchial biopsy specimens from a patient with severe asthma (A) and a normal control subject (B). C, Immunostaining for IL-11 in individual with severe asthma. D, Colocalization of IL-11 immunoreactivity (brown) and eosinophil-specific marker (MBP, red staining) in a subject with severe asthma. Journal of Allergy and Clinical Immunology 2007 119, 1563-1566DOI: (10.1016/j.jaci.2007.03.040) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Photomicrographs show IL-17 protein and mRNA expression. IL-17 immunostaining in purified blood eosinophils (A), sputum (B), and bronchoalveolar lavage fluid (C) from a subject with asthma; arrows indicate immunostained eosinophils. Positive hybridization for IL-17 mRNA of purified blood eosinophils from a subject with asthma (D). Journal of Allergy and Clinical Immunology 2007 119, 1563-1566DOI: (10.1016/j.jaci.2007.03.040) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Detection of IL-6 mRNA in human eosinophils. Subcellular localization of IL-6 mRNA in human eosinophils stimulated with IFN-γ (500 U/mL), stained with carbo1 chromotrope 2R (A), or immunostained simultaneously using EG2 and alkaline phosphatase-antialkaline phosphatase (B,arrow). Adapted from Hamid Q, Barkans J, Meng Q, Ying S, Abrams JS, Kay AB, et al. Human eosinophils synthesize and secrete interleukin-6, in vitro. Blood 1992;80:1496-501. Journal of Allergy and Clinical Immunology 2007 119, 1563-1566DOI: (10.1016/j.jaci.2007.03.040) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Immunocytochemistry showing basal expression of IL-25 in freshly purified peripheral blood eosinophils from a donor with asthma (A) and a control subject (B). Eosinophils from the subject with asthma show increased immunoreactivity for IL-25 compared with control. Journal of Allergy and Clinical Immunology 2007 119, 1563-1566DOI: (10.1016/j.jaci.2007.03.040) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 7 Immunofluorescence demonstrating expression of the IL-25 receptor (IL-17BR) in cultured ASMCs. Detection of IL-17BR in cultured ASMCs by immunofluorescence using an Alexa Fluor 555–labeled anti–IL-17BR antibody (Molecular Probes, Eugene, Ore) (A) versus isotype control (B). Magnification ×200. Journal of Allergy and Clinical Immunology 2007 119, 1563-1566DOI: (10.1016/j.jaci.2007.03.040) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions