The use of hyperosmotic saline for chondroprotection: implications for orthopaedic surgery and cartilage repair N.M. Eltawil, S.E.M. Howie, A.H.R.W.

Slides:

Advertisements

Similar presentations

Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen antibody-induced.

Advertisements

Extra-virgin olive oil diet and mild physical activity prevent cartilage degeneration in an osteoarthritis model: an in vivo and in vitro study on lubricin.

Muscle cell-derived factors inhibit inflammatory stimuli-induced damage in hMSC- derived chondrocytes R.S. Rainbow, H. Kwon, A.T. Foote, R.C. Preda, D.L.

Single-stage cell-based cartilage repair in a rabbit model: cell tracking and in vivo chondrogenesis of human umbilical cord blood-derived mesenchymal.

The impact of early intra-articular administration of interleukin-1 receptor antagonist on lubricin metabolism and cartilage degeneration in an anterior.

Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen antibody-induced.

Granulocyte macrophage – colony stimulating factor (GM-CSF) significantly enhances articular cartilage repair potential by microfracture M.-D. Truong,

Subchondral plate porosity colocalizes with the point of mechanical load during ambulation in a rat knee model of post-traumatic osteoarthritis H. Iijima,

Synovial mesenchymal stem cells from osteo- or rheumatoid arthritis joints exhibit good potential for cartilage repair using a scaffold-free tissue engineering.

D.A. Houston, A.K. Amin, T.O. White, I.D.M. Smith, A.C. Hall

Micromechanical mapping of early osteoarthritic changes in the pericellular matrix of human articular cartilage R.E. Wilusz, S. Zauscher, F. Guilak

Deiodinase 2 upregulation demonstrated in osteoarthritis patients cartilage causes cartilage destruction in tissue-specific transgenic rats H. Nagase,

Effects of short-term gentle treadmill walking on subchondral bone in a rat model of instability-induced osteoarthritis H. Iijima, T. Aoyama, A. Ito,

The groove model of osteoarthritis applied to the ovine fetlock joint

H. Moriyama, Ph. D. , O. Yoshimura, Ph. D. , S. Kawamata, Ph. D. , K

Cell deformation behavior in mechanically loaded rabbit articular cartilage 4 weeks after anterior cruciate ligament transection S.M. Turunen, S.-K.

L.N. Nwosu, P.I. Mapp, V. Chapman, D.A. Walsh

K. Murata, N. Kanemura, T. Kokubun, T. Fujino, Y. Morishita, K

Subchondral plate porosity colocalizes with the point of mechanical load during ambulation in a rat knee model of post-traumatic osteoarthritis H. Iijima,

Parathyroid hormone [1-34] improves articular cartilage surface architecture and integration and subchondral bone reconstitution in osteochondral defects.

Acute joint pathology and synovial inflammation is associated with increased intra- articular fracture severity in the mouse knee J.S. Lewis, W.C. Hembree,

Effects of short-term gentle treadmill walking on subchondral bone in a rat model of instability-induced osteoarthritis H. Iijima, T. Aoyama, A. Ito,

A.J. McGregor, B.G. Amsden, S.D. Waldman Osteoarthritis and Cartilage

Increase in excitatory amino acid concentration and transporters expression in osteoarthritic knees of anterior cruciate ligament transected rabbits

M. Ishikawa, K. Yoshioka, K. Urano, Y. Tanaka, T. Hatanaka, A. Nii

Toward scaffold-based meniscus repair: effect of human serum, hyaluronic acid and TGF-ß3 on cell recruitment and re-differentiation U. Freymann, M. Endres,

Acute mobilization and migration of bone marrow-derived stem cells following anterior cruciate ligament rupture T. Maerz, M. Fleischer, M.D. Newton,

Comparing non-enhanced and enhanced sequences in the assessment of effusion and synovitis in knee OA: associations with clinical, macroscopic and microscopic.

PGE2 signal via EP2 receptors evoked by a selective agonist enhances regeneration of injured articular cartilage S. Otsuka, M.D., T. Aoyama, M.D., Ph.D.,

X. Zhu, Y. Tang, J. Chen, S. Xiong, S. Zhuo, J. Chen

The layered structure of the articular surface

A novel exogenous concentration-gradient collagen scaffold augments full-thickness articular cartilage repair T. Mimura, M.D., S. Imai, M.D., M. Kubo,

Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that.

G. Venne, S. Pang, Y. Tse, R. Ellis Osteoarthritis and Cartilage

Destabilization of the medial meniscus leads to subchondral bone defects and site- specific cartilage degeneration in an experimental rat model H. Iijima,

Suppression of early experimental osteoarthritis by in vivo delivery of the adenoviral vector-mediated NF-κBp65-specific siRNA L.X. Chen, Ph.D., L. Lin,

The chemokine receptor CCR5 plays a role in post-traumatic cartilage loss in mice, but does not affect synovium and bone K. Takebe, M.F. Rai, E.J. Schmidt,

Oxidative stress induces expression of osteoarthritis markers procollagen IIA and 3B3(−) in adult bovine articular cartilage I.M. Khan, Ph.D., S.J. Gilbert,

Upregulation of lipocalin-2 (LCN2) in osteoarthritic cartilage is not necessary for cartilage destruction in mice W.-S. Choi, J.-S. Chun Osteoarthritis.

The anti-NGF antibody muMab 911 both prevents and reverses pain behaviour and subchondral osteoclast numbers in a rat model of osteoarthritis pain L.

Viability and volume of in situ bovine articular chondrocytes—changes following a single impact and effects of medium osmolarity Dr Peter G. Bush, Ph.D.,

A. Ludin, J.J. Sela, A. Schroeder, Y. Samuni, D.W. Nitzan, G. Amir

The chondrogenic repair response of undifferentiated mesenchymal cells in rat full- thickness articular cartilage defects Y. Anraku, M.D., H. Mizuta,

Exercise intervention increases expression of bone morphogenetic proteins and prevents the progression of cartilage-subchondral bone lesions in a post-traumatic.

Aerobic exercise training and low-level laser therapy modulate inflammatory response and degenerative process in an experimental model of knee osteoarthritis.

A histological comparison of the repair tissue formed when using either Chondrogide® or periosteum during autologous chondrocyte implantation H.S. McCarthy,

Synovial mesenchymal stem cells from osteo- or rheumatoid arthritis joints exhibit good potential for cartilage repair using a scaffold-free tissue engineering.

Repair of osteochondral defects with recombinant human type II collagen gel and autologous chondrocytes in rabbit H.J. Pulkkinen, V. Tiitu, P. Valonen,

A.C. Dang, M.D., A.P. Warren, M.D., H.T. Kim, M.D., Ph.D.

Deletion of 12/15-lipoxygenase accelerates the development of aging-associated and instability-induced osteoarthritis L. Habouri, F.E. El Mansouri, Y.

Lentiviral vector-mediated shRNAs targeting a functional isoform of the leptin receptor (Ob-Rb) inhibit cartilage degeneration in a rat model of osteoarthritis

Changes in the metabolism of chondroitin sulfate glycosaminoglycans in articular cartilage from patients with Kashin–Beck disease M. Luo, J. Chen, S.

Rapid in situ chondrocyte death induced by Staphylococcus aureus toxins in a bovine cartilage explant model of septic arthritis I.D.M. Smith, J.P. Winstanley,

T. Kurth, M. Sc. , E. Hedbom, Ph. D. , N. Shintani, Ph. D. , M

Utility of T2 mapping and dGEMRIC for evaluation of cartilage repair after allograft chondrocyte implantation in a rabbit model J. Endo, A. Watanabe,

Quantitative MR T2 measurement of articular cartilage to assess the treatment effect of intra-articular hyaluronic acid injection on experimental osteoarthritis.

Bisphosphonate rescues cartilage from trauma damage by promoting mechanical sensitivity and calcium signaling in chondrocytes Y. Zhou, M. Park, L. Wang,

Evidence for articular cartilage regeneration in MRL/MpJ mice

Growth characterization of neo porcine cartilage pellets and their use in an interactive culture model Carsten Lübke, Ph.D., Jochen Ringe, M.Sc., Veit.

An experimental study on costal osteochondral graft

Osteoarthritis development in novel experimental mouse models induced by knee joint instability S. Kamekura, M.D., K. Hoshi, M.D., Ph.D., T. Shimoaka,

S.I. Paterson, A.K. Amin, A.C. Hall Osteoarthritis and Cartilage

Cartilaginous repair of full-thickness articular cartilage defects is induced by the intermittent activation of PTH/PTHrP signaling S. Kudo, H. Mizuta,

The effects of alendronate in the treatment of experimental osteonecrosis of the hip in adult rabbits J.G. Hofstaetter, M.D., J. Wang, M.D., Ph.D., J.

A novel in vivo murine model of cartilage regeneration

Increased chondrocyte sclerostin may protect against cartilage degradation in osteoarthritis B.Y. Chan, E.S. Fuller, A.K. Russell, S.M. Smith, M.M. Smith,

Identification of molecular markers for articular cartilage

Microfracture and bone morphogenetic protein 7 (BMP-7) synergistically stimulate articular cartilage repair A.C. Kuo, M.D., Ph.D., J.J. Rodrigo, M.D.,

Estrogen reduces mechanical injury-related cell death and proteoglycan degradation in mature articular cartilage independent of the presence of the superficial.

Correlation between the MR T2 value at 4

Presentation transcript:

The use of hyperosmotic saline for chondroprotection: implications for orthopaedic surgery and cartilage repair N.M. Eltawil, S.E.M. Howie, A.H.R.W. Simpson, A.K. Amin, A.C. Hall Osteoarthritis and Cartilage Volume 23, Issue 3, Pages 469-477 (March 2015) DOI: 10.1016/j.joca.2014.12.004 Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Articular cartilage injury model in the rat knee joint. (A) H&E (left) toluidine blue (middle) and CMFDA with PI (right) staining of sham (lower panel) and injured (upper panel) rat articular cartilage. Arrows indicate the injury site. (B and C) Measurements of cartilage thickness, depth, width of the defect (B) and the percentage of injury depth to cartilage thickness under different irrigation strategies (C). Data are expressed as mean ± 95% CI; N = 10. Cartilage injury was induced by a single pass of a number 11 scalpel blade (see text for details) in the absence of irrigation solution (no irrigation; NI) or in the presence of normal saline (NS, 300 mOsm) or hyperosmolar saline (HS, 600 mOsm). Scale bar in (A) = 50 μm. Osteoarthritis and Cartilage 2015 23, 469-477DOI: (10.1016/j.joca.2014.12.004) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 In situ chondrocyte death after scalpel-induced injury. (A) A ROI was created in the axial CLSM image and live cells (marked in purple) and dead cells (marked in yellow) were identified and counted based on voxel intensity. (B) Axial CLSM projections of CMFDA and PI labelled injured chondrocytes within articular cartilage that was exposed to no irrigation (NI), normal saline (NS) or hyperosmolar saline (HS) at the indicated time points. (C and D) The PCD at day 0 (C) and at later time points (D) in injured and sham-operated joints exposed to different irrigation conditions. Data are expressed as mean ± 95% CI. P values are given in text. Scale bar in (B) = 100 μm. Osteoarthritis and Cartilage 2015 23, 469-477DOI: (10.1016/j.joca.2014.12.004) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Histological assessment of the changes to articular cartilage following scalpel-induced injury. (A) H&E (left) and toluidine blue (right) staining of sections from injured and sham-operated rat knee joints at the indicated time points. Arrows indicate areas of hypo-cellularity. (B) Polarized light microscopy of sections from sham and injured joints 8 weeks after injury. Arrows identify the site of injury and asterisks indicate the area of poor collagen organization. (C) Wakitani repair score at the 8 week time point. Significantly improved repair score was observed in non-irrigated joints (NI) and joints irrigated with hyperosmolar saline (HS) as compared to joints irrigated with normal saline (NS). Data are expressed as mean ± 95% CI; N = 5, P values are given in text. Scale bar in (A) and (B) = 50 μm. Osteoarthritis and Cartilage 2015 23, 469-477DOI: (10.1016/j.joca.2014.12.004) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Collagen and aggrecan immunoreactivity. Immunostaining for type II collagen (A) and aggrecan (B) in sham and injured articular cartilage 8 weeks after surgery. Injured articular cartilage was exposed to no irrigation (NI), normal saline (NS) or hyperosmolar saline (HS) before and after injury. Arrows identify the site of injury and asterisks indicate the area of reduced staining. (C and D) The area of reduced type II collagen (C) and aggrecan (D) in injured cartilage at 8 week time point. Data are expressed as mean ± 95% CI. P values are given in text. Scale bar in (A) and (B) = 50 μm. Osteoarthritis and Cartilage 2015 23, 469-477DOI: (10.1016/j.joca.2014.12.004) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Quantitative measurements of the width of the injury in the axial view. (A) CLSM projections of CMFDA and PI labelled injured articular cartilage that was exposed to no irrigation (NI), normal saline (NS) or hyperosmolar saline (HS) at Day 0 and 8 weeks after injury. (B) The width of the defect at day 0 and week 8. Data are expressed as mean ± 95% CI. P values are given in text. Scale bar in (A) = 50 μm. Osteoarthritis and Cartilage 2015 23, 469-477DOI: (10.1016/j.joca.2014.12.004) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Assessment of the inflammatory response following different irrigation conditions. (A) Representative H&E stained histological sections of the synovial membrane from injured knee joints exposed to no irrigation (NI), normal saline (NS) or hyperosmolar saline (HS) at 1 day, 1 week and 8 weeks after surgery. (B) Synovitis score in different irrigation conditions at 1 day, 1, 2 and 8 weeks post-surgery. (C) Cytokine array of serum samples obtained from control un-operated and experimental rats 1 day after injury. (D) IL-1α ELISA of serum samples from control and experimental rats at 1 day, 1, 2 and 8 weeks following cartilage injury. Data are expressed as mean ± 95% CI. N = 5. Scale bar in (A) = 200 μm. Osteoarthritis and Cartilage 2015 23, 469-477DOI: (10.1016/j.joca.2014.12.004) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions