A single DNA vaccination of C57BL/6 mice induced Ab responses of IgG1 and/or IgG2c subclass. A single DNA vaccination of C57BL/6 mice induced Ab responses.

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A single DNA vaccination of C57BL/6 mice induced Ab responses of IgG1 and/or IgG2c subclass. A single DNA vaccination of C57BL/6 mice induced Ab responses of IgG1 and/or IgG2c subclass. Each mouse was vaccinated with 25 μg DNA i.m. in each quadriceps followed by electroporation of the injection site. Serum obtained at various time points were analyzed by ELISA for HA-specific total IgG (A), IgG1 (B), and IgG2c (C) for the indicated targeted DNA vaccines (n = 6, mean ± SEM). The individual titers for each mouse is shown for day 28 for total IgG (D), IgG1 (E), and IgG2c (F) anti-HA Abs by ELISA (n = 6, bars indicate mean ± SEM, *p < 0.05, **p < 0.01 compared with the nontargeted αNIP-HA group by Mann–Whitney two-tailed t test). Samples with titer <50 was given an endpoint titer of 1. (G) HA-specific IgG1/IgG2c ratio shown for individual mice. Mean ± SEM is indicated. Dotted line indicates IgG1/IgG2c ratio = 1. Ranveig Braathen et al. ImmunoHorizons 2018;2:38-53 Copyright © 2018 The Authors