Examination of the effect of procalcitonin on human leucocytes and the porcine isolated coronary artery J.X. Wei, A. Verity, M. Garle, R. Mahajan, V.

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Presentation transcript:

Examination of the effect of procalcitonin on human leucocytes and the porcine isolated coronary artery J.X. Wei, A. Verity, M. Garle, R. Mahajan, V. Wilson British Journal of Anaesthesia Volume 100, Issue 5, Pages 612-621 (May 2008) DOI: 10.1093/bja/aen073 Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

Fig 1 Comparison of the effect of procalcitonin (PCT) 100 nM and LPS 10 μg ml−1 on forward scatter values for (a) human neutrophils and (b) human monocytes. Forward scatter has been measured in arbitrary units and the values shown are the mean (sem) of observations on 14 individuals. *P<0.05 and **P<0.01 denote a statistically significant difference from control values. British Journal of Anaesthesia 2008 100, 612-621DOI: (10.1093/bja/aen073) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

Fig 2 Comparison of the effect of procalcitonin (PCT) 100 nM, CGRP 100 nM, and the combination of CGRP and procalcitonin on (a) human neutrophil forward scatter, (b) human monocyte forward scatter, (c) human neutrophil CD16 expression, and (d) human monocyte CD14 expression. Forward scatter, CD 16 FL1, and CD 14 FL1 values have been measured in arbitrary units and values shown are the mean (sem) of observations on five individuals. *A statistically significant difference (P<0.05) from either control values or CGRP alone values. British Journal of Anaesthesia 2008 100, 612-621DOI: (10.1093/bja/aen073) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

Fig 3 Comparison of the effect of various agents on intracellular calcium levels of isolated human granulocytes, as identified by the forward scatter/side scatter plot, and measured using FLUO-3 fluorescence. (a) Fluorescence–time plot for human granulocytes before (time bins D and E) and after (time bins F and G). Exposure to procalcitonin (PCT) 1 μM. Between D/E and F/G, the flow cytometer was stopped and procalcitonin was added to the cells 30 s before restarting the machine. Under control conditions, the majority of granulocytes were located in time bin E, but approximately 40% of the cells showed an increase in intracellular calcium (time bin F) within 30 s of the addition of procalcitonin. (b) The effect of IL-8, fMLP, procalcitonin, and CGRP on the percentage of human granulocytes that showed an increase in intracellular calcium. Values shown are the mean (sem) of observations in six subjects. British Journal of Anaesthesia 2008 100, 612-621DOI: (10.1093/bja/aen073) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

Fig 4 Representative digitized recording of the effect of non-cumulative addition of procalcitonin 10 nM and procalcitonin 100 nM on U46619-induced contractions of the porcine isolated coronary artery. Also shown are the response to KCl 60 mM and the effect of cumulative addition of CGRP after exposure to procalcitonin. British Journal of Anaesthesia 2008 100, 612-621DOI: (10.1093/bja/aen073) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

Fig 5 The effect of U46619 on of the porcine isolated coronary artery after overnight incubation at 37oC in the presence and absence of either LPS 100 μg ml−1 or a combination of procalcitonin (PCT) 100 nM and LPS 100 μg ml−1. (a) The effect of procalcitonin added 3 h before LPS, and (b) the effect of procalcitonin added 3 h after LPS. Responses to U46619 have been determined as the force of contraction (g wt) and expressed as the mean (sem) of 14 observations. British Journal of Anaesthesia 2008 100, 612-621DOI: (10.1093/bja/aen073) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions