Flow cytometric analysis of putative progenitor cells.

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Flow cytometric analysis of putative progenitor cells. Flow cytometric analysis of putative progenitor cells. Representative dot plots of negative controls and stained samples are shown in red and blue respectively. First, leucocytes were identified on the basis of their characteristic forward and side scatter profile (A). CD34-FITC (B and C) expression and the proportion of CD34+ events expressing CD18 (D and E) and CXCR-4 (F and G) were determined. Similarly CD133-PE+ (H and I), CD45-PercP+ (J and K), and VEGFR-2-APC+ (L and M) events were identified. Co-expression of surface markers was determined using Boolean principles. In separate analyses CD14-FITC+ events were identified (N), and those expressing Tie-2-APC and or VEGFR-2-PE were determined using quadrant analysis (O and P). Gates were set on single or unstained negative controls where appropriate. Gareth J Padfield et al. Open Heart 2014;1:e000047 ©2014 by British Cardiovascular Society