Untreated Aphidicolin Wt Ctrl Wt Cre Atrflox Ctrl Atrflox Cre BRDU

Slides:

Advertisements

Similar presentations

Supplementary Figure S1Wang et. al. Supplementary Figure S1: (a) Purity of transferred Tconv. Tconv (CD4 + CD45RB hi CD25 ) were sorted from the spleen.

Advertisements

P-ERK β-tubulin VSShh + cyclopamine Supplementary Figure ESM 1a ERK MAP Kinase activity is not affected by Shh pathway activity. Western blot analysis.

Supplementary Figure 1. Suzuki et al. DAPI/BrdU BrdU 24 hrs 48 hrs 72 hrs.

BMT Wildtype mice GFP BM Markers used APC-Tcrb=marker of mature T-cells PE-TexasRed-CD19=marker of mature B- cells mature and also some of their.

Farmer et al Supplementary Figure 1 KU PARP-1 IC 50 = 3.2nM KU PARP-1 IC 50 = 3.4nM KU PARP-1 IC 50 = 730nM a b

Shimura Supplemental Figure 1

GFP-RV IFN/GFP-RV GFP-RV IFN/GFP-RV A B CD 8 C CD 8 GFP GFP GFP-RV

Figure S3 Young Cells Aged Cells *

Supplemental Figures A B % Senescent Cells Relative to control *

Figure 1 C. LDH release D. Cell viability E. Cell proliferation

Ctrl IR, 2h G2 P53 Ser15 S G1 G2 S G1 Figure S1. The bivariate (phospho-p53 Ser15 vs DNA content) distribution of control and irradiated with 6 Gy mESCs.

Supplementary Fig. 2 A B ** newly generated ISL1+ motor neurons (%)

Foxp3 induction dependent on cell cycle progression in vitro.

Tamoxifen induced deletion of FAK

Combination therapy for adult T-cell leukemia–xenografted mice: flavopiridol and anti-CD25 monoclonal antibody by Meili Zhang, Zhuo Zhang, Carolyn K. Goldman,

Supplemental Figure 8. Enterocyte migration and proliferation was dependent on host nutritional status of rotavirus-infected mice. A, Immunohistochemistry.

Control SC236(5mM) SC236(5mM) SC236(25mM) SC236(25mM)

Modulation of KRASG12C activity alters ARS-853 target engagement and supports novel therapeutic strategies for targeting KRAS. A, schematic for altering.

ICSBP/IRF-8 inhibits mitogenic activity of p210 Bcr/Abl in differentiating myeloid progenitor cells by Tomohiko Tamura, Hee Jeong Kong, Chainarong Tunyaplin,

SUPPLEMENTAL FIGURE 3. Probiotics increased enterocyte migration and proliferation in a strain-specific manner in rotavirus-infected normal weight mice.

Supplemental Figure 4. Probiotics increased enterocyte migration and proliferation in a strain-specific manner in rotavirus-infected overweight mice. A,

Supplemental Figure S1 a b

by Arun T. Kamath, Sandrine Henri, Frank Battye, David F

H31m1-PDL1 cells form progressively growing tumors in WT mice.

SUPPLEMENTAL TABLE 1. Cell cycle profiles of HeLa cells treated

Young Aged Isochronic Heterochronic Isochronic Heterochronic

Highly Efficient Selection of CD4 and CD8 Lineage Thymocytes Supports an Instructive Model of Lineage Commitment Andrea Itano, Ellen Robey Immunity

BrdU/Albumin/Hoechst

Qiuping He, Suwei Gao, Junhua Lv, Wei Li, Feng Liu

Supplemental Figure 2. Probiotics failed to increase enterocyte migration and proliferation in rotavirus-infected underweight mice. A, Immunohistochemistry.

Distinct pathways affected by menin versus MLL1/MLL2 in MLL-rearranged acute myeloid leukemia Yufei Chen, Kenneth L. Jones, Konstantinos Anastassiadis,

Heatmaps of differential OTUs

Supplementary Figure 1 a b c i ii iii i ii iii i ii iii

BCL‐2 overexpression does not confer protection to DKO cells after etoposide‐induced apoptosis. BCL‐2 overexpression does not confer protection to DKO.

Volume 42, Issue 1, Pages (January 2015)

Smoc2 marks intestinal stem cells in vivo.

CD facilitates RCT in vivo and promotes urinary cholesterol excretion

High proteasomal activity is involved in the palbociclib‐induced G1 arrest High proteasomal activity is involved in the palbociclib‐induced G1 arrest AMCF7.

Re‐expression of the AXIN1 coding sequence re‐sensitizes VACO6R cells to PORCN inhibition Re‐expression of the AXIN1 coding sequence re‐sensitizes VACO6R.

Transection of the chorda tympani depletes acinar cells at 7 days

Production of Mapk14 and Mapk7 KO cells.

UCN‐01 inhibits human IPF lung fibroblast pathological phenotypes and attenuates lung fibrosis induced by bleomycin injury UCN‐01 inhibits human IPF lung.

IL-27 contributes to IR expression by lung T cells during toxoplasmosis. IL-27 contributes to IR expression by lung T cells during toxoplasmosis. (A) Il27p28-GFP.

SLAM Family Markers Resolve Functionally Distinct Subpopulations of Hematopoietic Stem Cells and Multipotent Progenitors Hideyuki Oguro, Lei Ding, Sean J.

Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.

Arrest of Giardia cell cycle with aphidicolin and nocodazole.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Defective Cell Cycle Checkpoint Functions in Melanoma Are Associated with Altered Patterns of Gene Expression William K. Kaufmann, Kathleen R. Nevis,

BAP1 is required for homeostatic and antigen-driven expansion of peripheral T cells. BAP1 is required for homeostatic and antigen-driven expansion of peripheral.

Tcf4 is highly expressed and Tcf4GFPCre+neo genetically labels muscle connective tissue fibroblasts in culture. Tcf4 is highly expressed and Tcf4GFPCre+neo.

TNFα is an important survival and growth signal for melanoma.

Ectopic Pax7 increases MyoD expression in Pax7-null myogenic cells.

Altered luminal pH of the Golgi apparatus in GPHR deficient primary cultured neurons. Altered luminal pH of the Golgi apparatus in GPHR deficient primary.

VIP treatment decreased the number of apoptotic cells and increased cell proliferation. VIP treatment decreased the number of apoptotic cells and increased.

COX-2 deletion in macrophages led to increased renal macrophage infiltration in diabetic mice. COX-2 deletion in macrophages led to increased renal macrophage.

Proapoptotic Function of the Retinoblastoma Tumor Suppressor Protein

Supplementary figure 1 A B Water (Control) Pre-alcohol Post-alcohol

A Role for Proapoptotic BID in the DNA-Damage Response

Gab3 is required for IL-2– and IL-15–induced priming and expansion of NK cells. Gab3 is required for IL-2– and IL-15–induced priming and expansion of NK.

Fig. 4 Prematurely short S-phase and replication-associated DNA damage in p57KIP2-deficient fetal liver. Prematurely short S-phase and replication-associated.

PD-L1 expressed on edited T3 sarcoma cells prevents their immune elimination. PD-L1 expressed on edited T3 sarcoma cells prevents their immune elimination.

Pdcd4 expression inhibits AP-1 transactivation.

Characterization of Lkb1-WT and Lkb1-null cell lines.

Mice with a B cell–specific deletion of Ets1 do not have increased CD4+ T cell activation. Mice with a B cell–specific deletion of Ets1 do not have increased.

MET activation confers resistance to cetuximab (Cmab) or panitumumab (Pmab) in colon cancer cell lines in vitro and in vivo. MET activation confers resistance.

Knockdown of ROR1 increases the invasive potential of melanoma cells in vitro and in vivo. Knockdown of ROR1 increases the invasive potential of melanoma.

WEE1 inhibition followed by TRAIL treatment results in apoptotic cell death in MB231 cells. WEE1 inhibition followed by TRAIL treatment results in apoptotic.

Fig. 3 Ect2 gene inactivation lowers cardiomyocyte endowment and is lethal in mice. Ect2 gene inactivation lowers cardiomyocyte endowment and is lethal.

Yun-Gui Yang, Tomas Lindahl, Deborah E. Barnes Cell

**** *** * **** **** *** Shahriary et al.- Supplementary Figure 4 (a)

Presentation transcript:

Untreated Aphidicolin Wt Ctrl Wt Cre Atrflox Ctrl Atrflox Cre BRDU S/G2/M: 69% + 2 BrdU+: 3% + 0.2 S/G2/M: 47% + 1 Wt Ctrl BrdU+: 76% + 5 S/G2/M: 73% + 4 BrdU+: 3% + 2 S/G2/M: 47% + 1 Wt Cre BrdU+: 72% + 8 S/G2/M: 68% + 1 BrdU+: 3% + 0.4 S/G2/M: 46% + 3 Atrflox Ctrl BrdU+: 71% + 5 S/G2/M: 66% + 3 BrdU+: 1.2% + 0.1 S/G2/M: 47% + 1 Atrflox Cre BRDU 7-AAD Supplementary Figure S3. Cell cycle status of ATR-deleted fibroblasts. Subconfluent fibroblast cultures from Atrflox/- mice or wildtype B6 mice were transduced with MSCV-IRES-GFP or MSCV-Cre-IRES-GFP. Cells were cultured for 4 days, splitting as necessary, and replated for 1 day before treatment with 4 M aphidicolin. Before treatment and 16 hours after addition of aphidicolin cells were labeled with 10 M BrdU for 75 minutes and examined for BrdU incorporation and DNA content as described (Tough, D. F. & Sprent, J J. Exp. Med. 179, 1127–1135 (1994)). Raulet et al (MS 2004-12-27903B-Z)