This Month in Gastroenterology Jan Tack, John Carethers  Gastroenterology  Volume 136, Issue 5, Pages 1467-1470 (May 2009) DOI: 10.1053/j.gastro.2009.03.029 Copyright © 2009 AGA Institute Terms and Conditions

Figure 1 (A) Abdominal imaging in a patient with functional gut disorder during bloating shows anterior abdominal wall protrusion and diaphragmatic descent during bloating compared with basal with only a small increase (by 22 mL) in gas content. (B) Abdominal imaging in a patient with intestinal dysmotility during bloating shows marked increment in gas content (by 3352 mL) associated with anterior abdominal wall protrusion and abdominal ascent. Gastroenterology 2009 136, 1467-1470DOI: (10.1053/j.gastro.2009.03.029) Copyright © 2009 AGA Institute Terms and Conditions

Figure 2 Kaplan–Meier plots displaying time-to-hypoxemia with capnography blinded and open arm (A), subgrouped into ERCP and EUS (B). Gastroenterology 2009 136, 1467-1470DOI: (10.1053/j.gastro.2009.03.029) Copyright © 2009 AGA Institute Terms and Conditions

Figure 3 TTP antagonizes the ability of HuR overexpression to promote COX-2 expression. Expression constructs containing full-length COX-2 (COX-2+3′untranslated region), Flag-tagged HuR, and Flaf-tagged TTP were transfected into COS7 cells. The amount of COX-2 and HuR constructs were kept constant at 0.2 and 0.4 μg, respectively. Increasing amounts of TTP construct were transfected using empty vector to keep total DNA transfected at 1 μg. COX-2, Flag-TTP, Flag-HuR, and β-actin were detected by Western blot. Gastroenterology 2009 136, 1467-1470DOI: (10.1053/j.gastro.2009.03.029) Copyright © 2009 AGA Institute Terms and Conditions

Figure 4 Effect of lentiviral transduction of shRNA directed against Notch1 on the proportion of β-cells. Transduction of the cells with shNotch1 induced a significant increase in the proportion of beta cells (n = 4; #P = .041 compared with shScrambled condition). Treatment with the Notch ligands Jagged1 or Dll4 before knockdown emphasized the effect of the RNA interference with shNotch on β-cell differentiation (n = 4; #P = .009 compared with shScrambled condition). In conditions treated with the Notch1 extracellular domain (Notch-EC), subsequent RNA interference has no additional effect (n = 4; P = .12). Gastroenterology 2009 136, 1467-1470DOI: (10.1053/j.gastro.2009.03.029) Copyright © 2009 AGA Institute Terms and Conditions