Effect of oocyte vitrification on deoxyribonucleic acid methylation of H19, Peg3, and Snrpn differentially methylated regions in mouse blastocysts Ke-Ren.

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Effect of oocyte vitrification on deoxyribonucleic acid methylation of H19, Peg3, and Snrpn differentially methylated regions in mouse blastocysts Ke-Ren Cheng, Ph.D., Xiang-Wei Fu, Ph.D., Rui-Na Zhang, Ph.D., Gong-Xue Jia, Ph.D., Yun-Peng Hou, Ph.D., Shi-En Zhu, Ph.D. Fertility and Sterility Volume 102, Issue 4, Pages 1183-1190.e3 (October 2014) DOI: 10.1016/j.fertnstert.2014.06.037 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Representative methylation status of H19, Peg3, and Snrpn differentially methylated regions in oocytes. Each row represents a unique DNA clone; filled and open circles represent methylated and unmethylated CpGs, respectively. Fertility and Sterility 2014 102, 1183-1190.e3DOI: (10.1016/j.fertnstert.2014.06.037) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Methylation status of H19, Peg3, and Snrpn differentially methylated regions in blastocysts derived from fresh and vitrified oocytes. (A) H19; (B) Peg3; (C) Snrpn. Each row represents a unique methylation profile within the pool of clones sequenced. Filled and open circles represent methylated and unmethylated CpGs, respectively. The percentage on the right of the strand represents the proportion of strand with that phenotype. N represents the total number of selected clones. Fertility and Sterility 2014 102, 1183-1190.e3DOI: (10.1016/j.fertnstert.2014.06.037) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) Relative expression levels of DNMT mRNA in fresh and vitrified oocytes after thawing. In Dnmt3l, the error bars are too small to be observed. (B) Relative expression levels of DNMT mRNA in blastocysts derived from fresh and vitrified oocytes. Different superscripts represent statistically significant differences between groups (P<.05). Fertility and Sterility 2014 102, 1183-1190.e3DOI: (10.1016/j.fertnstert.2014.06.037) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions