Viktoria Konya, MSc, Eva M

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Presentation transcript:

Endothelium-derived prostaglandin I2 controls the migration of eosinophils Viktoria Konya, MSc, Eva M. Sturm, PhD, Petra Schratl, PhD, Eckhard Beubler, PhD, Gunther Marsche, PhD, Rufina Schuligoi, PhD, Irmgard Th. Lippe, PhD, Bernhard A. Peskar, MD, Akos Heinemann, MD Journal of Allergy and Clinical Immunology Volume 125, Issue 5, Pages 1105-1113 (May 2010) DOI: 10.1016/j.jaci.2009.12.002 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 PGI2 inhibits the migration of eosinophils through activation of IP receptors and adenylyl cyclase. The migration of human purified eosinophils (A and B) or neutrophils (C) toward eotaxin, C5a, or IL-8 was investigated in the presence of PGI2. D, Eosinophils were pretreated with vehicle, the IP antagonist CAY10441, or the adenylyl cyclase inhibitor SQ22536, and migration toward eotaxin was determined in the presence of PGI2. E and F, Expression of IP receptors on purified eosinophils was investigated by means of flow cytometry or Western blotting. Eo1-3, Eosinophils from 3 donors; PL, platelets. n = 4-8. ∗P < .05 versus vehicle. Journal of Allergy and Clinical Immunology 2010 125, 1105-1113DOI: (10.1016/j.jaci.2009.12.002) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 PGI2 inhibits eosinophil binding to fibronectin and attenuates the upregulation and activation of the adhesion molecule CD11b in eosinophils. A, Adhesion to fibronectin-coated 96-well plates was stimulated by eotaxin, and results were expressed as the portion of adherent cells relative to eosinophils added. B, Representative micrographs of eosinophils in the absence or presence of eotaxin (3 nmol/L) and PGI2 (100 nmol/L) adhering to fibronectin. Cells were stained with phalloidin–Texas Red and the nuclear dye DAPI (blue). C, Eotaxin-induced upregulation of CD11b is shown as a fold increase of baseline expression. D, Flow cytometric staining with isotype control antibody or mAb 24, an antibody directed against activated CD11b/CD18, of eosinophils incubated in the absence or presence of eotaxin (3 nmol/L), PGI2 (100 nmol/L), or both. n = 4-8. ∗P < .05, PGI2 versus vehicle (Veh). Journal of Allergy and Clinical Immunology 2010 125, 1105-1113DOI: (10.1016/j.jaci.2009.12.002) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Endogenous prostaglandins attenuate the adhesion of eosinophils to endothelial cells. Endothelial cells were pretreated with vehicle (Veh) or the COX inhibitor diclofenac, or eosinophils were pretreated with the IP antagonist CAY10441 or its vehicle. Eosinophils were added to the cultures in the absence or presence of various concentrations of eotaxin (A and B) or PGD2 (C and D). In Fig 3, D, Data from Fig 3, C, were replotted as the percentage increase above baseline (ie, in the presence of diclofenac or its vehicle but in the absence of eotaxin or PGD2). n = 6-8. ∗P < .05 versus vehicle. Journal of Allergy and Clinical Immunology 2010 125, 1105-1113DOI: (10.1016/j.jaci.2009.12.002) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Inhibition of prostaglandin synthesis disturbs the barrier function of endothelial monolayers and enhances the transmigration of eosinophils. Endothelial cells that had been cultured in Transwell inserts until confluence were pretreated with vehicle or the COX inhibitor diclofenac (diclo; 10 μmol/L) for 2 or 5 hours. Thereafter, eosinophils were added to the top wells and were allowed to transmigrate for 4 hours. A, Transendothelial electrical resistance was measured before and after migration and was expressed as a percentage of baseline value (ie, before diclofenac was added). B, Eosinophils that had migrated to the bottom wells were enumerated by means of flow cytometry, and data were expressed as a percentage of cells that had migrated toward eotaxin through cell-free filters. n = 3-6. ∗P < .05, diclofenac versus vehicle of diclofenac. Journal of Allergy and Clinical Immunology 2010 125, 1105-1113DOI: (10.1016/j.jaci.2009.12.002) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Fluorescence staining of VE-cadherin and F-actin reveals that inhibition of prostaglandin biosynthesis disintegrates the texture of endothelial monolayers. Endothelial cells that had been cultured until confluence were pretreated with vehicle, the COX inhibitor diclofenac (10 μmol/L), or diclofenac plus iloprost (100 nmol/L) and were then stained with control antibody (small insert), anti–VE-cadherin antibody (green), or phalloidin–Texas Red and the nuclear stain DAPI (blue). The stainings shown are representative of 3 independent experiments. Journal of Allergy and Clinical Immunology 2010 125, 1105-1113DOI: (10.1016/j.jaci.2009.12.002) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions