Fixation and embedding

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Presentation transcript:

Fixation and embedding

Staining Hematoxylin & Eosin Periodic acid Schiff Toluidine blue Silver

Light microscopy

Inverted microscope

Phase contrast microscope

Differential interference contrast (DIC) component

Electron microscope scanning transmission

Epithelium

Connective tissue

Glycosaminoglycan

Collagen

Collagen Type Molecule Structural Features Representative Tissues Composition Fibrillar Collagens I [α1(I)]2[α2(I)] 300-nm-long fibrils Skin, tendon, bone, ligaments, dentin, interstitial tissues II [α1(II)]3 300-nm-long fibrils Cartilage, vitreous humor III [α1(III)]3 300-nm-long fibrils; Skin, muscle, blood vessels often with type I V [α1(V)]3 390-nm-long fibrils with Similar to type I; also cell globular N-terminal cultures, fetal tissues domain; often with type I Fibril-Associated Collagens VI [α1(VI)][α2(VI)] Lateral association with Most interstitial tissues type I; periodic globular domains IX [α1(IX)][α2(IX)] Lateral association with Cartilage, vitreous humor [α3(IX)] type II; N-terminal globular domain; bound glycosaminoglycan Sheet-Forming Collagens IV [α1(IV)]2[α2(IV)] Two-dimensional network All basal laminaes

Bone

Bone marrow

Hematopoiesis

Neuron

Synapse

Sarcoplasmic reticulum

Take home message Neuronal structure Neuromuscular junction