Ervin Macas, Min Xie, Sara Schaufelberger, Gabriele S

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Presentation transcript:

Vitrification of human single pronuclear oocytes following two approaches to polar body biopsy Ervin Macas, Min Xie, Sara Schaufelberger, Gabriele S. Merki-Feld, Ruth Stiller, Bruno Imthurn Reproductive BioMedicine Online Volume 22, Issue 4, Pages 376-381 (April 2011) DOI: 10.1016/j.rbmo.2011.01.004 Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 The mean total cell number (TCN) of blastocysts developed from all three groups of vitrified single pronuclear oocytes and the control group of non-vitrified single pronuclear oocytes. The difference in TCN of blastocysts developed in the laser-treated group and the non-vitrified control was significantly different (P<0.028). PZD=partial zona dissection. Reproductive BioMedicine Online 2011 22, 376-381DOI: (10.1016/j.rbmo.2011.01.004) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Differential staining of human blastocyst developed from human single pronuclear oocyte. The intense pink colour (propidium iodide and bisbenzimide) represents the chromatin in nuclei of lysed trophectoderm cells. The blue colour (bisbenzimide) represents nuclei of inner cell mass. The arrow depicts the fragmented nucleus of a dead cell. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Reproductive BioMedicine Online 2011 22, 376-381DOI: (10.1016/j.rbmo.2011.01.004) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions