Shao Jun Jiang, Sang Min Hwang, Eung Ho Choi, Sung Ku Ahn

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Presentation transcript:

Structural and Functional Effects of Oleic Acid and Iontophoresis on Hairless Mouse Stratum Corneum Shao Jun Jiang, Sang Min Hwang, Eung Ho Choi, Sung Ku Ahn Journal of Investigative Dermatology Volume 114, Issue 1, Pages 64-70 (January 2000) DOI: 10.1046/j.1523-1747.2000.00834.x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 TEWL after application of 0.3 M OA in different concentrations of PG for 2 h. Animals were treated with 0.3 M OA in different concentrations of 95, 90, 80, 70, 60, and 50% of PG in distilled water, and absolute OA, respectively. The elevation of TEWL was dependent on the concentration of PG. Results are represented as mean (± SEM). *p < 0.05 versus the TEWL after application of absolute OA; NS, nonsignificant. Journal of Investigative Dermatology 2000 114, 64-70DOI: (10.1046/j.1523-1747.2000.00834.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Changes of TEWL after application of different concentrations of OA according to application times. Groups were treated with 0.1, 0.15, 0.2, and 0.3 M OA in 90% PG and 90% PG only, each for 1, 1.5, 2, 3, and 4 h. When 90% PG was used as the vehicle, no significant changes of TEWL occurred. After treatment in different concentrations of OA, TEWL increased in relation to the concentration of OA and application time. Results are represented as mean (± SEM). *p < 0.05, **p < 0.001 versus the TEWL treated with 90% PG by an analysis of variance. Journal of Investigative Dermatology 2000 114, 64-70DOI: (10.1046/j.1523-1747.2000.00834.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 TEWL after application of 0.3 M OA for 1 h. Animals were received iontophoresis alone (+ or – electrode); topical application of OA covered with Tegaderm; iontophoretic patch containing OA; Latex occlusion with OA and iontophoresis with OA, respectively. TEWL was not changed after iontophoresis alone, and increased 2-fold after application of OA with iontophoretic patch without power supply, of levels are similar to topical application of OA which covered both Tegaderm and Latex occlusion. In contrast, the TEWL was increased markedly with a combination of iontophoresis and OA. Results are represented as mean (± SEM). *p < 0.01, **p < 0.001 versus normal TEWL, and ***p < 0.001 versus TEWL after 1 h application with Tegaderm iontophoresis patch, and Latex by an analysis of variance. Journal of Investigative Dermatology 2000 114, 64-70DOI: (10.1046/j.1523-1747.2000.00834.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Recovery rates of TEWL after topical application of 0.3 M OA for 2 h and by iontophoresis with OA for 1 h. After 4 h post-treatment, the barrier function recovered by nearly 50%. There were no differences in TEWL recovery in comparison with after tape-stripping. TEWL rates are plotted as the percentage recovery of TEWL. Each point represents the mean (± SEM). Journal of Investigative Dermatology 2000 114, 64-70DOI: (10.1046/j.1523-1747.2000.00834.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Two hours treatment of absolute PG and OA in hairless mice. (A) With PG treatment, foci of individual membranes separated by amorphous materials can be seen (A, arrowheads). (B) Displays after 2 h OA treatment in mouse skin. Note the uniform preservation of the intercellular lipid lamellae (arrows) and presence of lacunae (asterisks). Scale bar: 200 nm. Journal of Investigative Dermatology 2000 114, 64-70DOI: (10.1046/j.1523-1747.2000.00834.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Ultrastructure of topical 0.3 M OA in PG/distilled water (9:1) treated epidermis. After 1 h application, normal-appearing lamellar membrane (A, inset), and phase-separated lamellar membrane structure (arrowheads) as well as lacunae (asterisk) coexist within SC (A). By 2 h treatment, the formation of lacunae (stars), and variable amounts of amorphous material (asterisks) filling in the widened SC interstices (B). These alterations are considerably more remarkable than those present in after 1 h 0.3 M OA-treated sample (cf. A). Scale bar: 200 nm. Journal of Investigative Dermatology 2000 114, 64-70DOI: (10.1046/j.1523-1747.2000.00834.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 Electron micrographs of iontophoresis for 1 h. (A) Shows the normal-appearing lipid lamellae. This sample is taken from the animal treated with the iontophoretic patch without a power supply for 1 h. (B) Displays the sample taken from beneath of positive electrode, whereas (C) is from the negative electrode. The structures of lipid lamellae appear fragmentary in many sites (single arrows) and flocculent materials are within SC interstices (asterisks) as well as the appearance of lamellar phase separation (B). Scale bar: 200 nm. Journal of Investigative Dermatology 2000 114, 64-70DOI: (10.1046/j.1523-1747.2000.00834.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 8 Ultrastructural observation of SC after iontophoresis with 0.3 M OA for 1 h, which demonstrates the alterations in lipid membrane structure leading to barrier abnormality. The dilated lacunae (stars), electron-dense, and amorphous relatively electron-lucent material are observed in the widened intercellular spaces of SC (asterisks). (A) Positive electrode; (B) negative electrode. Scale bar: 200 nm. Journal of Investigative Dermatology 2000 114, 64-70DOI: (10.1046/j.1523-1747.2000.00834.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 9 Forty-eight hours post-OA treatment. At this stage of near-complete barrier repair, accompanied the presence of normal lipid lamellae within the intercellular space of SC. Scale bar: 200 nm. Journal of Investigative Dermatology 2000 114, 64-70DOI: (10.1046/j.1523-1747.2000.00834.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions