rSema4A induces iTreg cells in CD4+ T cell cultures.

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rSema4A induces iTreg cells in CD4+ T cell cultures. rSema4A induces iTreg cells in CD4+ T cell cultures. CD4+ T cells were isolated from PBMCs by negative selection as described in the legend to Fig. 7 and depleted of natural Treg cells using CD25 microbeads. CD4+CD25depleted cells were stimulated with plate-bound anti-CD3 Ab plus soluble anti-CD28 Ab and cultured for 6 d in cRPMI supplemented with IL-2 and/or TGF-β with or without rSema4A. The relative numbers of CD4+CD25+Foxp3+ T cells were assessed in three flow cytometry settings for each cell culture condition, and the corresponding statistics were evaluated. The median relative number of iTreg cells ± SEM in three culture settings is shown below each density plot. Note that rSema4A was equally efficient to TGF-β1 in iTreg cell induction. *p < 0.006, IL-2 + TGF-β versus either TGF-β or IL-2 alone. **p < 0.035, Sema4A versus IL-2. ***p < 0.02, IL-2 + Sema4A versus IL-2 alone. Svetlana P. Chapoval et al. ImmunoHorizons 2019;3:71-87 Copyright © 2019 by The Authors