Fig. 5. Stimulation of EPO expression and secretion under hypoxic culture conditions. Stimulation of EPO expression and secretion under hypoxic culture.

Slides:

Advertisements

Similar presentations

Glioma-derived soluble factor(s) enhance IL-10 signaling.

Advertisements

Fig. 1 CSF1 is increased in blood of melanoma patients and correlates with disease progression. CSF1 is increased in blood of melanoma patients and correlates.

Fig. 1. TP is highly expressed in myeloma.

IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.

Fig. 7. Downstream effects of α-synuclein on mitochondria.

Fig. 1. IL-6 is associated with resistance to EGFR TKIs and is induced by stress hormones. IL-6 is associated with resistance to EGFR TKIs and is induced.

Differentiation of AZD4785 from MAPK pathway inhibitors in vitro

Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.

Fig. 5. Blocking LTB4 during initial lymphangiogenesis period abrogates the therapeutic benefit of LTB4 antagonism. Blocking LTB4 during initial lymphangiogenesis.

Transfer of miR-223 during neutrophil-epithelial cell interactions

Fig. 2. Pharmacologic inhibition of ALK impairs STING activation.

Fig. 2. Mechanism of PD-L1 down-regulation in NOD HSPCs.

Fig. 8. Gene and protein changes in ALK-dependent STING pathways in human sepsis. Gene and protein changes in ALK-dependent STING pathways in human sepsis.

Fig. 1. BCAS1 expression identifies newly generated oligodendrocytes.

Fig. 2. In vitro profiling of tEV markers on cell line–derived EVs.

Fig. 1. Schematic representation of the MANO method.

PVSRIPO-mediated APC activation occurs in immunosuppressive conditions

Fig. 3. Serum HBsAg and liver HBV mRNA reduction in chimpanzees dosed with ARC-520. Serum HBsAg and liver HBV mRNA reduction in chimpanzees dosed with.

Fig. 8. mRIPO elicits neutrophil influx followed by DC and T cell infiltration into tumors. mRIPO elicits neutrophil influx followed by DC and T cell infiltration.

Fig. 3 TLR8 signaling induces CXCL4 and IFN-α secretion by SSc PDCs.

Fig. 7. The PD-L1 defect is evident in HSPCs from T1D patients.

Bio-AMS enhances the activity of rifampicin and ethambutol in vitro

Expression of CD36 and psap in a TMA of human ovarian cancer patients

Fig. 3. Wisper controls CF behavior and survival.

Fig. 8. Therapeutic effects of the transplantation of hiPSC-EPO–producing cells on renal anemia in adenine-treated mice. Therapeutic effects of the transplantation.

Fig. 4 CXCL4 potentiates TLR8-mediated activation of SSc pDCs.

Fig. 3. Applying the rapid test to analyze human patient sera.

HIF-1α is critically involved in hypoxia-induced CD137 upregulation.

Fig. 5 Hypoxic tumors from obese mice associate with increased production of IL-6 by adipocytes and myeloid cells. Hypoxic tumors from obese mice associate.

Fig. 2. Circulating VEGF in GCA patients up-regulates microvascular endothelial Jagged1. Circulating VEGF in GCA patients up-regulates microvascular endothelial.

Persistence of CAR4 cells is reduced after sustained TCR engagement

Fig. 1. mGlu7 expression is reduced in RTT autopsy samples.

Fig. 4. Genetically engineered PD-L1

Fig. 3. β-AR signaling induces IL-6 in NSCLC cells via activation of PKC and CREB. β-AR signaling induces IL-6 in NSCLC cells via activation of PKC and.

Fig. 5 CXCL4 potentiates TLR9-mediated activation but has minimal effect on TLR7-mediated activation of pDCs purified from SSc or HDs. CXCL4 potentiates.

Fig. 3. ERRα is involved in the regulation of OPLAH.

Fig. 2 Fas controls IL-1RA–sEV secretion in murine MSCs.

Fig. 1 BX795 suppresses HSV-1 infection.

Fig. 7. Therapeutic effects of hiPSC-EPO protein on renal anemia in adenine-treated mice. Therapeutic effects of hiPSC-EPO protein on renal anemia in adenine-treated.

Fig. 5. Nutlin-3 treatment rescues the proliferation and differentiation of NPCs in vitro. Nutlin-3 treatment rescues the proliferation and differentiation.

Fig. 4 TNF-α up-regulates Fas/Fap-1 expression to promote IL-1RA–sEV release in murine MSCs. TNF-α up-regulates Fas/Fap-1 expression to promote IL-1RA–sEV.

N H N H Figure S1 parp-1 +/+ parp-1 -/- HIF-2 DAPI

Fig. 2. IL-2/rapamycin–expanded T cells express homing receptors to traffic to lymphoma sites and are resistant to SN-38 toxicity. IL-2/rapamycin–expanded.

The selective PI3K inhibitor A66 suppresses PIP3 accumulation, AKT phosphorylation at Thr308, and YAP/TAZ–regulated gene expression in PDAC cells. The.

Bexarotene is neuroprotective in mouse and human HD neurons in vitro

Fig. 4. Acute lung injury in miR-223−/y mice.

Fig. 5 DMN-Tre labeling is selective for live mycobacteria.

Fig. 1. Specificity of FolamiR uptake in cancer cells in culture.

Fig. 6 DMF inhibits NF-κB translocation upon infection.

Fig. 3 Activation of freshly isolated CD4+ T cells from HIV-infected patients on ART selectively reverses baseline blocks to splicing, elongation, and.

CSF1 secretion by melanoma cells is induced by CTL-derived cytokines

Identification of Z-FA-FMK as a potent compound that increases SMN protein expression in HEK293 and patient fibroblast cells. Identification of Z-FA-FMK.

Effect of insulin on hepcidin expression in HepG2 cells.

Fig. 2 AcPGP induces IL-8 and G-CSF release from human bronchial epithelial cells. AcPGP induces IL-8 and G-CSF release from human bronchial epithelial.

Fig. 1. PARN is differentially expressed in breast cancer versus non-cancerous tissue/cells. PARN is differentially expressed in breast cancer versus non-cancerous.

Effects of Chd1-knockdown on Hmgpi and Klf5 expression

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

Caveolin-1 downregulated survivin expression in cells with enhanced or constitutive activity via the Wnt pathway. Caveolin-1 downregulated survivin expression.

CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured SGBS cells during adipocyte differentiation. CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured.

Aβ-mediated Ras-MAPK signaling and Cyclin D1 expression in B103 cells are dependent on APP expression and can be reversed with MEK or Ras inhibition. Aβ-mediated.

HIF-1 is required for IGF-1-induced VEGF mRNA expression and protein secretion. HIF-1 is required for IGF-1-induced VEGF mRNA expression and protein secretion.

APLF regulates genes implicated in MET during the generation of iPSCs from fibroblasts. APLF regulates genes implicated in MET during the generation of.

Human pluripotent stem cell–derived erythropoietin-producing cells ameliorate renal anemia in mice by Hirofumi Hitomi, Tomoko Kasahara, Naoko Katagiri,

Bcl-XL upregulates the expression of genes characteristic to midbrain and hindbrain fates during in vitro differentiation of ES cells. Bcl-XL upregulates.

PX-478 inhibits hypoxic HIF-1 protein expression and signaling activity in a dose-dependent manner. PX-478 inhibits hypoxic HIF-1 protein expression and.

Gramicidin A (GA) decreases HIF protein expression in RCC cells.

Secretion of TNF-α from macrophages and monocytic cells were reduced by the deletion of Nrdc. Secretion of TNF-α from macrophages and monocytic cells were.

Fig. 4. miR promotes cardiomyocyte proliferation through regulation of Hippo pathway kinases. miR promotes cardiomyocyte proliferation through.

Fig. 2 Increasing KLF17, CDH1, and LASS2 expression reduced malignant progression and promoted apoptosis of tumor cells. Increasing KLF17, CDH1, and LASS2.

Fig. 2. Mechanism of PD-L1 down-regulation in NOD HSPCs.

Presentation transcript:

Fig. 5. Stimulation of EPO expression and secretion under hypoxic culture conditions. Stimulation of EPO expression and secretion under hypoxic culture conditions. (A and B) Time-course analyses of EPO mRNA expression (A) and protein secretion (B) in hiPSC-EPO cells cultured under low oxygen (1%) conditions compared to control hiPSC-EPO cells cultured under normal oxygen (21%) conditions. EPO mRNA expression was measured using qRT-PCR, whereas the EPO protein concentrations in the culture medium were analyzed using ELISA. Each value was normalized to that of samples on day 0 in (A). (C and D) AFP (C) and ALBUMIN (D) mRNA expression was analyzed using qRT-PCR. Each value was normalized to control stage 2 hiPSC-EPO cells on culture day 8 cultured under normoxic conditions. (E and F) The effects of PHD inhibitors on EPO mRNA expression (E) and protein secretion (F) in HepG2 cells and in stage 2 hiPSC-EPO cells on culture day 8 were analyzed using qRT-PCR and ELISA, respectively (100 μM DFO, 50 μM FG4592, and 1 mM DMOG). Each value was normalized to control samples (no PHD inhibitor) in (E). (G) Effects of an HIF-1 dimerization inhibitor, Acriflavine (10 μM), on EPO protein secretion by hiPSC-EPO cells. (H) The nuclear translocation of HIF-1α and HIF-2α in hiPSC-EPO and HepG2 cells under hypoxic (1%) conditions was evaluated by immunocytochemistry. The data from four independent experiments are means ± SEM. *P < 0.05 versus control samples under normoxic conditions in (A) and (B) or without PHD inhibitors in (E) to (G); **P < 0.05 versus control samples (HepG2 cells) in (E) and (F); ANOVA with Bonferroni’s test (A, B, E, and F) and Student’s t test (G). Scale bars, 20 μm (H). Hirofumi Hitomi et al., Sci Transl Med 2017;9:eaaj2300 Published by AAAS