Optimal dose of basic fibroblast growth factor for long-segment orthotopic tracheal autografts Ryoichi Nakanishi, MD, Nobuyuki Nagaya, MD, Takashi Yoshimatsu,

Slides:

Advertisements

Similar presentations

Development and regression of intimal thickening of arterially transplanted autologous vein grafts in dogs Kazumasa Morinaga, M.D., Hiroshi Eguchi, M.D.,

Advertisements

Experimental study on a new tracheal prosthesis made from collagen-conjugated mesh Norihito Okumura, MD, Tatsuo Nakamura, MD, Tohru Natsume, MD, Kenji.

Vascular endothelial growth factor expression in non-small-cell lung cancer: Prognostic significance in squamous cell carcinoma Hideyuki Imoto, MD, Toshihiro.

David D. Yuh, MD, Kimberly L. Gandy, MD, Bruce A

Tracheal regeneration: Myth or fact?

Implantable sensor for intraoperative and postoperative monitoring of blood flow: A preliminary report Raphael S. Rabinovitz, PhD, Craig J. Hartley,

Tracheal Replacement With a Silicone-Stented, Fresh Aortic Allograft in Sheep Hisashi Tsukada, MD, PhD, Armin Ernst, MD, Sidhu Gangadharan, MD, Simon.

Experimental tracheal allograft revascularization and transplantation

The use of technetium tc 99m annexin V for in vivo imaging of apoptosis during cardiac allograft rejection Patrick W. Vriens, MDa, Francis G. Blankenberg,

In situ tissue engineering for tracheal reconstruction using a luminar remodeling type of artificial trachea Tatsuo Nakamura, MD, Toshihiko Sato, Masato.

Tracheal Regeneration Following Tracheal Replacement With an Allogenic Aorta Emmanuel Martinod, MD, PhD, Agathe Seguin, MD, Muriel Holder-Espinasse, MD,

Is Tracheal Transplantation Possible With Cryopreserved Tracheal Allograft and Hyperbaric Oxygen Therapy? An Experimental Study Fatih Candas, MD, Rauf.

Tracheal regeneration: Evidence of bone marrow mesenchymal stem cell involvement Agathe Seguin, MD, PhD, Sonia Baccari, MD, Muriel Holder-Espinasse, MD,

Morphologic Assessment of Extracellular Matrix Scaffolds for Patch Tracheoplasty in a Canine Model Thomas W. Gilbert, PhD, Sebastien Gilbert, MD, Matthew.

Nodular pulmonary amyloidosis

Arteriotomy closure by glued patch in the porcine carotid artery

Angiogenic response induced by mechanical transmyocardial revascularization Victor Chu, MDa, Jin-qiang Kuang, MDa, Amy McGinna, Adel Giaid, PhDb, Stephen.

Protection of rat spinal cord from ischemia with dextrorphan and cycloheximide: Effects on necrosis and apoptosis Hiroyuki Kato, MD, PhDa c, Georgios.

Behavior of vital and killed autologous pericardium in the descending aorta of sheep David T. Cheung, PhD, Suk Jung Choo, MD, Albert C. Grobe, MS, Douglas.

Right internal thoracic artery or saphenous vein grafting

Two-Stage End-to-End Reconstruction of Long-Segment Tracheal Defects With a Bioabsorbable Scaffold Grafting Technique in a Canine Model Hisashi Tsukada,

Makoto Hashizume, M. D. , Ph. D. , Yeng Yang, M. D. , Spencer Galt, M

First human transplantation of a bioengineered airway tissue

Clifford H. Van Meter, MD (by invitation), William C

Intrapulmonary benign fibrous tumor of the pleura

Biodegradable polymer coating promotes the epithelization of tissue-engineered airway prostheses Toshihiko Sato, MD, Masato Araki, MD, Naoki Nakajima,

Experimental study of a new sutureless intraluminal graft with a shape-memory alloy ring Chuanrui Yang, MD, Yanqing Sun, MD, Peiqing Dong, MD, Huazhong.

Multilevel data analysis: What? Why? How?

Conformational stress and anastomotic hyperplasia

Surgical treatment of isolated left anterior descending coronary stenosis: Comparison of left internal mammary artery and venous autograft at 18 to 20.

Martin H. Chamberlain, FRCS, David P. Taggart, MD, FRCS

The prognostic significance of tumor cell detection in intraoperative pleural lavage and lung tissue cultures for patients with lung cancer J. Buhr,

Editorial: The core of leadership

Successful allotransplantation of cryopreserved tracheal grafts with preservation of the pars membranacea in nonhuman primates Tomohiro Murakawa, MD,

The correlation between tumor size and lymphatic vessel invasion in resected peripheral stage I non-small-cell lung cancer Yukito Ichinose, MDa, Tokujiro.

Construction of a bioengineered cardiac graft

Occult active giant cell aortitis necessitating surgical repair

Tsutomu Tagawa, MDa, Takashi Suda, MDa, Niccolò Daddi, MDa, Benjamin D

Tracheal allotransplantation in beagle dogs without immunosuppressants

Fresh venous allografts in peripheral arterial reconstruction in dogs

Intrathoracic tracheal reconstruction with a collagen-conjugated prosthesis: Evaluation of the efficacy of omental wrapping Masayoshi Teramachi, MDa,

Support Your Specialty

Antisense basic fibroblast growth factor alters the time course of mitogen-activated protein kinase in arterialized vein graft remodeling Akimasa Yamashita,

Carinal reconstruction with a Y-shaped collagen-conjugated prosthesis

Cold ischemia–induced damage to vascular endothelium results in permeability alterations in transplanted lungs M.A. Hidalgo, MDa b, K.A. Shah, MRCPatha,

Mark K. Hirko, MD, Joseph R. McShannic, MD, Steven P

Differential diagnosis of thymic carcinoma and lung carcinoma with the use of antibodies to cytokeratins Ichiro Fukai, MDa, Akira Masaoka, MD, PhDa,

Intraabdominal hemorrhage as a result of segmental mediolytic arteritis of an omental artery: Case report Dianne M. Heritz, MD *, Jagdish Butany, MD,

The effect of carbon coating and porosity on early patency of expanded polytetrafluoroethylene grafts: An experimental study Donald L. Akers, MD, Yong.

Late angiographic result of using the right gastroepiploic artery as a graft Hisayoshi Suma, MD, Tadashi Isomura, MD, Taiko Horii, MD, Toru Sato, MD

Increase in size of the pulmonary autograft after the ross operation in children: Growth or dilation? Laszlo Solymar, MD, PhD, Göran Südow, MD, Daniel.

Ara K. Pridjian, MD, Edward L. Bove, MD, Flavian M. Lupinetti, MD

Functional analysis of cryopreserved veins

H. Yokomise, MDa, K. Inui, MDa, H. Wada, MDa, M. Ueda, MDa, S

Histological confirmation of healing of gastrobronchial fistula using a muscle flap Manabu Okuyama, MD, Reijiro Saito, MD, Satoru Motoyama, MD, Michihiko.

Gelatin sheet incorporating basic fibroblast growth factor enhances sternal healing after harvesting bilateral internal thoracic arteries Atsushi Iwakura,

F. Henry Ellis, MD, PhD, Xiangjun Xu, MD, PhD, Matthew H

Carcinoma of the esophagus: Prognostic significance of histologic type

Ralph S. Mosca, MD The Journal of Thoracic and Cardiovascular Surgery

Mediastinal vagal neurilemmoma causing tracheal stenosis

P.K. Spanos, MD, A.D. Bisbos, MD, I.I. Arditis, MD

Richard L. Binns, MD Journal of Vascular Surgery

Ren-Ke Li, MD, PhD, Richard D. Weisel, MD, Donald A. G

Darrell N. Jones, PhD, Robert B

Thirty years of cardiac transplantation at stanford university

Hongcan Shi, MD, PhD, Hong Xu, MD, Dan Lu, MD, Jun Wu, MD

Replacement of a tracheal defect with a tissue-engineered prosthesis: Early results from animal experiments Jhingook Kim, MD, Soo Won Suh, PhD, Ji Yeon.

Quiz Page January 2010 American Journal of Kidney Diseases

Neointimal formation at the sites of anastomosis of the internal thoracic artery grafts after coronary artery bypass grafting in human subjects: An immunohistochemical.

Experimental study on a new tracheal prosthesis made from collagen-conjugated mesh Norihito Okumura, MD, Tatsuo Nakamura, MD, Tohru Natsume, MD, Kenji.

Presentation transcript:

Optimal dose of basic fibroblast growth factor for long-segment orthotopic tracheal autografts Ryoichi Nakanishi, MD, Nobuyuki Nagaya, MD, Takashi Yoshimatsu, MD, Takeshi Hanagiri, MD, Kosei Yasumoto, MD The Journal of Thoracic and Cardiovascular Surgery Volume 113, Issue 1, Pages 26-36 (January 1997) DOI: 10.1016/S0022-5223(97)70396-9 Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Bronchoscopic findings in canine tracheal autograft on postoperative day 370 in group IV (dog No. 14). A, Anastomotic site at oral end; B, midportion of graft; C, anastomotic site at caudal end. Graft was covered with glossy mucosa and showed good patency. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Bronchoscopic findings in canine tracheal autograft on postoperative day 370 in group IV (dog No. 14). A, Anastomotic site at oral end; B, midportion of graft; C, anastomotic site at caudal end. Graft was covered with glossy mucosa and showed good patency. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Bronchoscopic findings in canine tracheal autograft on postoperative day 370 in group IV (dog No. 14). A, Anastomotic site at oral end; B, midportion of graft; C, anastomotic site at caudal end. Graft was covered with glossy mucosa and showed good patency. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 2 Cumulative percent survival. Survival in group IV differed significantly from survival in other groups (group IV versus groups I and II, p = 0.0080; group IV versus groups III, V, and VI, p = 0.0286). Survival in groups III, V, and VI differed significantly from survival in groups I and II (p = 0.0055). Mean survival time of each group was as follows: groups I and II, 9 days; groups III, V, and VI, 33 days. Mean survival time for group IV was not measured because animals were killed. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 3 Canine tracheal autograft on postoperative day 12 in group I (dog No. 3). A, Gross findings; B, histologic findings (hematoxylin-eosin stain; original magnification ×12.5). Autograft is necrotic and cartilage is disintegrated or dissolved. Epithelial regeneration is not seen. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 3 Canine tracheal autograft on postoperative day 12 in group I (dog No. 3). A, Gross findings; B, histologic findings (hematoxylin-eosin stain; original magnification ×12.5). Autograft is necrotic and cartilage is disintegrated or dissolved. Epithelial regeneration is not seen. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 4 Canine tracheal autograft on postoperative day 393 in group IV (dog No. 14). A, Gross findings; B, histologic findings (hematoxylin and eosin stain; original magnification ×12.5); C, epithelial morphology (original magnification ×100). Tracheal continuity is restored by autograft. Many vessels and proliferation of submucosal connective tissue and cartilage are seen, but narrowing of graft is not seen. Epithelium is almost completely restored (89.1%) and is composed of nearly normal mucociliary epithelium (epithelial regeneration score = 2.24). The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 4 Canine tracheal autograft on postoperative day 393 in group IV (dog No. 14). A, Gross findings; B, histologic findings (hematoxylin and eosin stain; original magnification ×12.5); C, epithelial morphology (original magnification ×100). Tracheal continuity is restored by autograft. Many vessels and proliferation of submucosal connective tissue and cartilage are seen, but narrowing of graft is not seen. Epithelium is almost completely restored (89.1%) and is composed of nearly normal mucociliary epithelium (epithelial regeneration score = 2.24). The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 4 Canine tracheal autograft on postoperative day 393 in group IV (dog No. 14). A, Gross findings; B, histologic findings (hematoxylin and eosin stain; original magnification ×12.5); C, epithelial morphology (original magnification ×100). Tracheal continuity is restored by autograft. Many vessels and proliferation of submucosal connective tissue and cartilage are seen, but narrowing of graft is not seen. Epithelium is almost completely restored (89.1%) and is composed of nearly normal mucociliary epithelium (epithelial regeneration score = 2.24). The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 5 Average graft patency. Data are presented as mean plus or minus standard error. *, **, p < 0.05 versus group I at that time; #, ##, p < 0.05 versus group II at that point (graft patency 7 days after transplantation: groups I versus IV, p = 0.0009; I versus VI, p = 0.0177; II versus IV, p = 0.0009; II versus VI, p = 0.0305, and graft patency at observed end-point: groups I versus IV, p = 0.0055; I versus VI, p = 0.0124; II versus IV, p = 0.0004). Autografts in group IV showed good patency throughout entire postoperative course and were significantly superior to those in groups I and II, which did not receive bFGF. FG, Fibrin glue; rGEA, wrapping of omental flap fed by right gastroepiploic artery; rGEA + SA, omental wrapping fed by blood supplies from both right gastroepiploic and splenic arteries. Open bars represent graft patency 7 days after transplantation; solid bars represent patency at observed end-point. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 6 Number of blood vessels in tracheal grafts. Data are presented as mean plus or minus standard error. *, p < 0.05 versus group I; #, p < 0.05 versus group II (groups I versus IV, p = 0.0141; I versus V, p = 0.0210; I versus VI, p = 0.0011; II versus IV, p = 0.0141; II versus V, p = 0.0433; II versus VI, p = 0.0006). Autografts in group IV showed highest number of vessels and were significantly superior to those in all groups except for group VI. Vessel numbers of groups V and VI differed significantly from those of other three groups, except for group IV, but did not differ significantly from each other. FG, Fibrin glue; rGEA, wrapping of omental flap fed by right gastroepiploic artery; rGEA + SA, omental wrapping fed by blood supplies from both right gastroepiploic and splenic arteries. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 26-36DOI: (10.1016/S0022-5223(97)70396-9) Copyright © 1997 Mosby, Inc. Terms and Conditions