Volume 13, Issue 10, Pages (May 2003)

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Volume 13, Issue 10, Pages 843-848 (May 2003) Arabidopsis HEN1 Stéphanie Boutet, Franck Vazquez, Jun Liu, Christophe Béclin, Mathilde Fagard, Ariane Gratias, Jean-Benoit Morel, Patrice Crété, Xuemei Chen, Hervé Vaucheret Current Biology Volume 13, Issue 10, Pages 843-848 (May 2003) DOI: 10.1016/S0960-9822(03)00293-8

Figure 1 Phenotype and Virus Sensitivity of Line L1 and the 23-2 Mutant (A) Phenotype of line L1 and of the 23-2 mutant 2 weeks after mock infection or infection by CMV. All plants are the same age, indicating the delay in flowering of the 23-2 mutant. (B) A close-up of (A) showing the leaf phenotype of the 23-2 mutant. (C) CMV RNA accumulation in line L1 and in the 23-2 mutant, mock infected or infected by CMV. Standardization with a 25S rDNA probe is shown below. Current Biology 2003 13, 843-848DOI: (10.1016/S0960-9822(03)00293-8)

Figure 2 GUS mRNA and siRNA Accumulation in Wild-Type and Mutant Plants (A) mRNA extracted from leaves was hybridized with a GUS DNA probe. An ethidium bromide-stained gel is shown for standardization. The ratio between GUS and 25S signals is indicated below. (B) siRNA extracted from leaves was hybridized with antisense RNA probes corresponding to the 5′ part (position 1–558), central part (position 558–789), or 3′ part (position 789–1865) of the GUS coding sequence. Similar results were obtained with sense probes. Hybridization with 5S RNA is shown for standardization. The ratio between GUS and 5S signals is indicated below. Current Biology 2003 13, 843-848DOI: (10.1016/S0960-9822(03)00293-8)

Figure 3 miR171 miRNA and SCL mRNA Accumulation in Wild-Type and Mutant Plants (A) Small RNA extracted from wild-type and mutant flowers was hybridized with a probe complementary to miR171. (B) Total RNA extracted from wild-type and mutant flowers was quantified for SCL6-III mRNA relative accumulation by real-time PCR by using primers surrounding the cleavage site. Quantifications are normalized with actine2 transcript. The wild-type value is 1. AU, arbitrary unit. Current Biology 2003 13, 843-848DOI: (10.1016/S0960-9822(03)00293-8)