Effect of vitrification on human oocytes: a metabolic profiling study

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Presentation transcript:

Effect of vitrification on human oocytes: a metabolic profiling study Francisco Dominguez, Ph.D., Damià Castello, Ph.D., José Remohí, M.D., Carlos Simón, M.D., Ana Cobo, Ph.D. Fertility and Sterility Volume 99, Issue 2, Pages 565-572.e3 (February 2013) DOI: 10.1016/j.fertnstert.2012.09.034 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Principal component analysis (PCA) representing all the metabolites analyzed for each sample in the two rounds. Blue/green triangles represent fresh group, and red triangles represent vitrification group. No separation between groups is observed using (A, C) positive and (B, D) negative ionization of the samples analyzed in both rounds of analysis. Fertility and Sterility 2013 99, 565-572.e3DOI: (10.1016/j.fertnstert.2012.09.034) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Distributions of measurements in both fresh (green rhombus) and vitrification groups (red rhombus) of (B) tryptophan, (D) phenylalanine, (C) lactate, and (A) glucose. Fertility and Sterility 2013 99, 565-572.e3DOI: (10.1016/j.fertnstert.2012.09.034) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) Box plots of alpha-CEHC, a metabolite found to be statistically significant between fresh and vitrification groups using positive and negative ionization. Dots correspond to individual measurements of alpha-CEHC. (B) Receiver operating characteristic (ROC) curves are shown for this metabolite. Fertility and Sterility 2013 99, 565-572.e3DOI: (10.1016/j.fertnstert.2012.09.034) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Experimental design of the study. Microdrops were taken from 190 spent culture media from embryos that had been cultured from day 1 to day 3. Collection was performed on day 3 by collecting 50 μL of culture media. The samples were collected from the fresh group (n = 59), the vitrification group (n = 65), and matched control media group (n = 66) in the LC-MS study. Metabolomic analysis was performed in two rounds due to the instrument capacity limitations. Fertility and Sterility 2013 99, 565-572.e3DOI: (10.1016/j.fertnstert.2012.09.034) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions