Volume 115, Issue 1, Pages 173-176 (July 1998) Overexpression of an unstable intrinsic factor–cobalamin receptor in Imerslund– Gräsbeck syndrome  Deborah Murdoch Eaton*, John H. Livingston*, Bellur Seetharam‡, John W.L. Puntis*  Gastroenterology  Volume 115, Issue 1, Pages 173-176 (July 1998) DOI: 10.1016/S0016-5085(98)70379-8 Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 1 Ileal biopsy specimens from patients with Imerslund–Gräsbeck syndrome and ileal mucosa obtained from an unidentified donor were homogenized in Tris-HCl buffer (pH 7.4) containing 140 mmol/L NaCl and centrifuged at 100,000g for 1 hour. The resulting pellet was resuspended in the same buffer and homogenized. Total membrane protein (50 μg) containing 2 fmol (lane 1, control), 25 fmol (lane 2, patient 1), or 5 fmol (lane 3, patient 2), or 100 μg protein containing 50 fmol (lane 4, patient 1), 10 fmol (lane 5, patient 2), or 20 μg of total membranes from 14-day-old pregnant rat yolk sac containing 20 fmol of ligand binding activities (lane 6) were subjected to 5% sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The separated proteins were transferred to nitrocellulose membranes and probed with diluted antiserum to canine ileal IFCR and 125I–protein A as described previously.6,7 Gastroenterology 1998 115, 173-176DOI: (10.1016/S0016-5085(98)70379-8) Copyright © 1998 American Gastroenterological Association Terms and Conditions