Matrix metalloproteinases modulated by protein kinase Cε mediate resistin-induced migration of human coronary artery smooth muscle cells Qinxue Ding,

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Matrix metalloproteinases modulated by protein kinase Cε mediate resistin-induced migration of human coronary artery smooth muscle cells Qinxue Ding, PhD, Hong Chai, MD, PhD, Nausheen Mahmood, Jerry Tsao, Daria Mochly-Rosen, PhD, Wei Zhou, MD Journal of Vascular Surgery Volume 53, Issue 4, Pages 1044-1051 (April 2011) DOI: 10.1016/j.jvs.2010.10.117 Copyright © 2011 Terms and Conditions

Fig 1 In vitro effect of resistin on human coronary artery smooth muscle cell migration in Boyden chamber assay. Two concentrations of resistin at physiologic (10 ng/mL) and pathologic (40 ng/mL) concentrations were tested. TNF-α was used as a positive control. Values are expressed as the number of migrated cells, and each bar represents the mean ± SEM of quadruplicate determinations. Journal of Vascular Surgery 2011 53, 1044-1051DOI: (10.1016/j.jvs.2010.10.117) Copyright © 2011 Terms and Conditions

Fig 2 Effect of resistin on matrix metalloproteinase (MMP)-2 and MMP-9 expression in human coronary artery smooth muscle cells. Resistin was tested at physiologic (10 ng/mL) and pathologic (40 ng/mL) concentrations. Left panels, MMP-2 mRNA and protein data; right panels, MMP-9 mRNA and protein data. Values are expressed as percentage of the control, and each bar represents the mean ± SEM of triplicate determinations in two to three independent experiments. Journal of Vascular Surgery 2011 53, 1044-1051DOI: (10.1016/j.jvs.2010.10.117) Copyright © 2011 Terms and Conditions

Fig 3 Effect of resistin on tissue inhibitor metalloproteinase (TIMP) mRNA expression in human coronary artery smooth muscle cells. Resistin was tested at physiologic (10 ng/mL) and pathologic (40 ng/mL) concentrations. Left panel, TIMP-1 mRNA data; right panel, TIMP-2 mRNA data. Values are expressed as percentage of the control, and each bar represents the mean ± SEM of triplicate determinations in two to three independent experiments. Journal of Vascular Surgery 2011 53, 1044-1051DOI: (10.1016/j.jvs.2010.10.117) Copyright © 2011 Terms and Conditions

Fig 4 Effect of matrix metalloproteinase (MMP)-2 and MMP-9 antibodies on resistin-induced cell migration of human coronary artery smooth muscle cells (SMCs) (Boyden chamber assay). The migratory effect of resistin was exerted at the concentration of 40 ng/mL. Anti-MMP-2 and MMP-9 as well as nonspecific IgG were tested at the same concentration of 10 μg/mL. Values are expressed as the number of migrated cells, and each bar represents the mean ± SEM of quadruplicate determinations. Journal of Vascular Surgery 2011 53, 1044-1051DOI: (10.1016/j.jvs.2010.10.117) Copyright © 2011 Terms and Conditions

Fig 5 Effects of resistin on protein kinase C (PKC)ε activation and PKCε inhibitor (εV1-2) on resistin-induced PKCε activation. Values are expressed as percentage of the control, and each bar represents the mean ± SEM of duplicate determinations in two independent experiments. Journal of Vascular Surgery 2011 53, 1044-1051DOI: (10.1016/j.jvs.2010.10.117) Copyright © 2011 Terms and Conditions

Fig 6 Effect of protein kinase C (PKC)ε inhibitor on matrix metalloproteinase (MMP) and tissue inhibitor metalloproteinase (TIMP) mRNA expressions in human coronary artery smooth muscle cells. Resistin was tested at the concentration of 40 ng/mL. Top panels, MMP mRNA data; bottom panels, TIMP mRNA data. Values are expressed as percentage of the control, and each bar represents the mean ± SEM of triplicate determinations in two independent experiments. Journal of Vascular Surgery 2011 53, 1044-1051DOI: (10.1016/j.jvs.2010.10.117) Copyright © 2011 Terms and Conditions

Fig 7 Effect of protein kinase C (PKC)ε inhibitor on matrix metalloproteinase (MMP) activity in human coronary artery smooth muscle cells. Resistin was tested at the concentration of 40 ng/mL. Left panel, MMP-2 activity; right panel, MMP-9 activity. Values are expressed as percentage of the control, and each bar represents the mean ± SEM of duplicate determinations in two independent experiments. Journal of Vascular Surgery 2011 53, 1044-1051DOI: (10.1016/j.jvs.2010.10.117) Copyright © 2011 Terms and Conditions

Fig 8 Effect of protein kinase C (PKC)ε inhibitor on resistin-induced cell migration of human coronary artery smooth muscle cells (SMCs) (Boyden chamber assay). The migratory effect of resistin was exerted at the concentration of 40 ng/mL. Values are expressed as the number of migrated cells, and each bar represents the mean ± SEM of quadruplicate determinations. Journal of Vascular Surgery 2011 53, 1044-1051DOI: (10.1016/j.jvs.2010.10.117) Copyright © 2011 Terms and Conditions