Pnut is phosphorylated during early Drosophila embryogenesis.

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Pnut is phosphorylated during early Drosophila embryogenesis. Pnut is phosphorylated during early Drosophila embryogenesis. (A) Pnut protein was immunoprecipitated from the 0–2 hr embryonic extract using anti-Pnut antibody. Immunoprecipitated material was treated with λ phosphatase where indicated and analyzed by silver-stained SDS-PAGE. (B) Extracts from embryos at different time points of development were analyzed by immunoblot with anti-Pnut antibodies. (C) Extracts from different organs and tissues, as well as S2 tissue culture cells, were subjected to immunoblot using antibody against Pnut. (D) Schematic representation of Pnut protein structure. Phosphorylated in vivo residues (shown in red) and their relative locations are shown. Ab, antibody; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Katarina Akhmetova et al. G3 2017;8:27-38 ©2018 by Genetics Society of America