Spa typing directly from a mecA, spa and pvl multiplex PCR assay—a cost-effective improvement for methicillin-resistant Staphylococcus aureus surveillance

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Presentation transcript:

spa typing directly from a mecA, spa and pvl multiplex PCR assay—a cost-effective improvement for methicillin-resistant Staphylococcus aureus surveillance A.R. Larsen, M. Stegger, M. Sørum Clinical Microbiology and Infection Volume 14, Issue 6, Pages 611-614 (June 2008) DOI: 10.1111/j.1469-0691.2008.01995.x Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Examples of isolates typed by the spa, mecA and pvl multiplex PCR. Lanes: 1 and 12, 100-bp ladder; 2, H2O control; 3, Staphylococcus epidermidis DK-14; 4, S. epidermidis ATCC 51625; 5, methicillin-susceptible Staphylococcus aureus (MSSA) ATCC 6538; 6, MSSA DK-E2211; 7, methicillin-resistant Stapylococcus aureus (MRSA) ATCC 33591; 8, MRSA USA300-0114; 9, MRSA CC80 (282-01); 10, MRSA CC30 (3250-01); 11, MRSA DK-55599 (one spa repeat). Clinical Microbiology and Infection 2008 14, 611-614DOI: (10.1111/j.1469-0691.2008.01995.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions