Contrasting effects of G1. 2/G2

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Presentation transcript:

Contrasting effects of G1. 2/G2 Contrasting effects of G1.2/G2.2 and SOF1/SOF2 embryo culture media on pre- and post-implantation development of non-transgenic and transgenic cloned goat embryos Sayed Morteza Hosseini, Mehdi Hajian, Somayyeh Ostadhosseini, Mohsen Forouzanfar, Parvaneh Abedi, Farnoosh Jafarpour, Hamid Gourabi, Abdol Hossein Shahverdi, Ahmad Vosough, Hamid Reza Ghanaie, Mohammad Hossein Nasr-Esfahani Reproductive BioMedicine Online Volume 31, Issue 3, Pages 372-383 (September 2015) DOI: 10.1016/j.rbmo.2015.06.008 Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 In-vitro developmental competence of goat SCNT embryos with three cell lines cultured in SOF1/SOF2 and G1.2/G2.2. htPA = fetal cell line transfected with human tissue plasminogen activator; hrcfIX = fetal cell line transfected with human coagulation factor IX. Values with different letters are statistically significantly different at P < 0.05. Reproductive BioMedicine Online 2015 31, 372-383DOI: (10.1016/j.rbmo.2015.06.008) Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Effect of embryo culture medium on relative expression of 17 genes in blastocysts produced by somatic cell nuclear transfer (SCNT). SOF1/SOF2 was considered as reference (control) and mRNA levels in G1.2/G2.2 were determined relative to SOF1/SOF2. For each gene, bars with * superscripts were significantly different at P < 0.05. Reproductive BioMedicine Online 2015 31, 372-383DOI: (10.1016/j.rbmo.2015.06.008) Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Effect of embryo culture medium on relative expression of 17 genes in blastocysts produced by IVF. SOF1/SOF2 considered as reference (control) and mRNA levels in G1.2/G2.2 were determined relative to SOF1/SOF2. For each gene, bars with * superscripts were significantly different at P < 0.05. Reproductive BioMedicine Online 2015 31, 372-383DOI: (10.1016/j.rbmo.2015.06.008) Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions