The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the guinea pig V.B. Kraus, J.L. Huebner, J. DeGroot,

Slides:

Advertisements

Similar presentations

Comparison of mouse and human ankles and establishment of mouse ankle osteoarthritis models by surgically-induced instability S.H. Chang, T. Yasui, S.

Advertisements

B. Bai, Y. Li Osteoarthritis and Cartilage

Advances in combining gene therapy with cell and tissue engineering-based approaches to enhance healing of the meniscus M. Cucchiarini, A.L. McNulty,

Biochemical markers of type II collagen breakdown and synthesis are positioned at specific sites in human osteoarthritic knee cartilage A.-C. Bay-Jensen,

Subchondral plate porosity colocalizes with the point of mechanical load during ambulation in a rat knee model of post-traumatic osteoarthritis H. Iijima,

Oleuropein or rutin consumption decreases the spontaneous development of osteoarthritis in the Hartley guinea pig M.-N. Horcajada, C. Sanchez, F. Membrez.

Micromechanical mapping of early osteoarthritic changes in the pericellular matrix of human articular cartilage R.E. Wilusz, S. Zauscher, F. Guilak

Osteoporosis increases the severity of cartilage damage in an experimental model of osteoarthritis in rabbits E. Calvo, M.D., S. Castañeda, M.D., R.

Non-destructive evaluation of articular cartilage defects using near-infrared (NIR) spectroscopy in osteoarthritic rat models and its direct relation.

Maturation-dependent change and regional variations in acoustic stiffness of rabbit articular cartilage: an examination of the superficial collagen-rich.

Histopathological subgroups in knee osteoarthritis

J.L. Huebner, M.S., V.B. Kraus, M.D., Ph.D.

L.N. Nwosu, P.I. Mapp, V. Chapman, D.A. Walsh

An in vivo cross-linkable hyaluronan gel with inherent anti-inflammatory properties reduces OA cartilage destruction in female mice subjected to cruciate.

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in sheep and goats C.B. Little, M.M. Smith, M.A.

ADAMTS5−/− mice have less subchondral bone changes after induction of osteoarthritis through surgical instability: implications for a link between cartilage.

Initial application of EPIC-μCT to assess mouse articular cartilage morphology and composition: effects of aging and treadmill running N. Kotwal, J.

K. Murata, N. Kanemura, T. Kokubun, T. Fujino, Y. Morishita, K

Subchondral plate porosity colocalizes with the point of mechanical load during ambulation in a rat knee model of post-traumatic osteoarthritis H. Iijima,

Whole-body vibration of mice induces articular cartilage degeneration with minimal changes in subchondral bone M.R. McCann, C. Yeung, M.A. Pest, A. Ratneswaran,

Acute joint pathology and synovial inflammation is associated with increased intra- articular fracture severity in the mouse knee J.S. Lewis, W.C. Hembree,

C. -H. Chou, M. T. M. Lee, I. -W. Song, L. -S. Lu, H. -C. Shen, C. -H

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the horse C.W. McIlwraith, D.D. Frisbie, C.E.

Depletion of primary cilia in articular chondrocytes results in reduced Gli3 repressor to activator ratio, increased Hedgehog signaling, and symptoms.

Angiogenesis in two animal models of osteoarthritis

Transglutaminase 2 is a marker of chondrocyte hypertrophy and osteoarthritis severity in the Hartley guinea pig model of knee OA J.L. Huebner, K.A. Johnson,

Osteoarthritis-like changes in the heterozygous sedc mouse associated with the HtrA1– Ddr2–Mmp-13 degradative pathway: a new model of osteoarthritis D.W.

Cartilage damage pattern in relation to subchondral plate thickness in a collagenase- induced model of osteoarthritis S.M. Botter, M.Sc., G.J.V.M. van.

Spatial and temporal changes of subchondral bone proceed to microscopic articular cartilage degeneration in guinea pigs with spontaneous osteoarthritis

Comparing non-enhanced and enhanced sequences in the assessment of effusion and synovitis in knee OA: associations with clinical, macroscopic and microscopic.

The short-term therapeutic effect of recombinant human bone morphogenetic protein-2 on collagenase-induced lumbar facet joint osteoarthritis in rats

Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture.

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the rabbit S. Laverty, C.A. Girard, J.M. Williams,

Monoiodoacetic acid induces arthritis and synovitis in rats in a dose- and time- dependent manner: proposed model-specific scoring systems M. Udo, T.

Destabilization of the medial meniscus leads to subchondral bone defects and site- specific cartilage degeneration in an experimental rat model H. Iijima,

The chemokine receptor CCR5 plays a role in post-traumatic cartilage loss in mice, but does not affect synovium and bone K. Takebe, M.F. Rai, E.J. Schmidt,

Comparison of mouse and human ankles and establishment of mouse ankle osteoarthritis models by surgically-induced instability S.H. Chang, T. Yasui, S.

The anti-NGF antibody muMab 911 both prevents and reverses pain behaviour and subchondral osteoclast numbers in a rat model of osteoarthritis pain L.

Protective effects of a cathepsin K inhibitor, SB , in the canine partial medial meniscectomy model of osteoarthritis J.R. Connor, C. LePage, B.A.

Differences in structural and pain phenotypes in the sodium monoiodoacetate and meniscal transection models of osteoarthritis P.I. Mapp, D.R. Sagar,

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the rat N. Gerwin, A.M. Bendele, S. Glasson,

Metabolic enrichment of omega-3 polyunsaturated fatty acids does not reduce the onset of idiopathic knee osteoarthritis in mice A. Cai, E. Hutchison,

B.D. Bomsta, M.S., L.C. Bridgewater, Ph.D., R.E. Seegmiller, Ph.D.

3D histopathological grading of osteochondral tissue using contrast-enhanced micro- computed tomography H.J. Nieminen, H.K. Gahunia, K.P.H. Pritzker,

P. Julkunen, J. Iivarinen, P. A. Brama, J. Arokoski, J. S. Jurvelin, H

Exercise intervention increases expression of bone morphogenetic proteins and prevents the progression of cartilage-subchondral bone lesions in a post-traumatic.

Dietary fatty acids differentially affect the development of injury-induced osteoarthritis with diet-induced obesity in mice C.-L. Wu, D. Jain, D. Little,

P.C. Pastoureau, E.B. Hunziker, J.-P. Pelletier

M. A. McNulty, R. F. Loeser, C. Davey, M. F. Callahan, C. M

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the dog J.L. Cook, K. Kuroki, D. Visco, J.-P.

Changes of human menisci in osteoarthritic knee joints

Loss of Frzb and Sfrp1 differentially affects joint homeostasis in instability-induced osteoarthritis S. Thysen, F.P. Luyten, R.J. Lories Osteoarthritis.

Osteoarthritis development in novel experimental mouse models induced by knee joint instability S. Kamekura, M.D., K. Hoshi, M.D., Ph.D., T. Shimoaka,

Joint loading and proximal tibia subchondral trabecular bone microarchitecture differ with walking gait patterns in end-stage knee osteoarthritis B.C.

Opposing cartilages in the patellofemoral joint adapt differently to long-term cruciate deficiency: chondrocyte deformation and reorientation with compression

R. J. U. Lories, M. D. , Ph. D. , J. Peeters, B. Sc. , K. Szlufcik, Ph

Significance of the serum CTX-II level in an osteoarthritis animal model: a 5-month longitudinal study M.E. Duclos, O. Roualdes, R. Cararo, J.C. Rousseau,

Dose–response relationship for exercise on severity of experimental osteoarthritis in rats: a pilot study Laurent Galois, M.D., Stéphanie Etienne, M.Sc.,

K. Kuroki, C.R. Cook, J.L. Cook Osteoarthritis and Cartilage

Intra-articular therapy with recombinant human GDF5 arrests disease progression and stimulates cartilage repair in the rat medial meniscus transection.

J.L. Huebner, J.M. Williams, M. Deberg, Y. Henrotin, V.B. Kraus

Weight bearing as a measure of disease progression and efficacy of anti-inflammatory compounds in a model of monosodium iodoacetate-induced osteoarthritis

Quantitative pre-clinical screening of therapeutics for joint diseases using contrast enhanced micro-computed tomography N.J. Willett, T. Thote, M. Hart,

Weight-bearing asymmetry and vertical activity differences in a rat model of post- traumatic knee osteoarthritis C.B. Hamilton, M.A. Pest, V. Pitelka,

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the mouse S.S. Glasson, M.G. Chambers, W.B. Van.

A. Sophocleous, A.E. Börjesson, D.M. Salter, S.H. Ralston

M. L. Roemhildt, B. D. Beynnon, A. E. Gauthier, M. Gardner-Morse, F

Comparison of cartilage histopathology assessment systems on human knee joints at all stages of osteoarthritis development C. Pauli, R. Whiteside, F.L.

Cartilage degeneration in different human joints

B.D. Bomsta, M.S., L.C. Bridgewater, Ph.D., R.E. Seegmiller, Ph.D.

Presentation transcript:

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the guinea pig V.B. Kraus, J.L. Huebner, J. DeGroot, A. Bendele Osteoarthritis and Cartilage Volume 18, Pages S35-S52 (October 2010) DOI: 10.1016/j.joca.2010.04.015 Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Guinea pig knee joint structures. A frontal section through a 12-month-old guinea pig knee joint to demonstrate its structures and the landmarks and features typical of advanced OA at the center of the tibiofemoral joint (M=medial; L=lateral). Osteoarthritis and Cartilage 2010 18, S35-S52DOI: (10.1016/j.joca.2010.04.015) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 The timecourse of spontaneous histological OA in the Hartley guinea pig knee. (a) Representative sections of the central medial tibial plateau stained with toluidine blue for each age group (ages 2–18 months). Scores of cartilage structure (CS) and proteoglycan content (PG) using the recommended scoring system for the regions shown is as follows: 2 months CS=0/PG=0; 4 months CS=2/PG=1; 7 months CS=5/PG=4; 10 months CS=5/PG=5; 12 months CS=8/PG=5; and 18 months CS=8/PG=6. (b) Mean histological scores of the knee demonstrating increasing OA severity with age in this model system (N=6 for each group ages 2–12 months and N=10 for 18 month age group). Error bars=SD. Osteoarthritis and Cartilage 2010 18, S35-S52DOI: (10.1016/j.joca.2010.04.015) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Macroscopic images of the guinea pig knee joint. (a) Guinea pig knee joint demonstrating normal dimensions of approximately 1cm×1cm (width×depth). (b) Arial views of the tibial plateau oriented (medial on left, lateral on right) with macroscopic lesions of increasing severity from no lesions (left panel), to moderate surface fissuring (middle panel), and severe full depth erosion to bone (right panel); the diseased areas in the middle and right panels are indicated by the dotted demarcated region. Osteoarthritis and Cartilage 2010 18, S35-S52DOI: (10.1016/j.joca.2010.04.015) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Landmarks of the central region of the guinea pig tibiofemoral joint. (a) A schematic representation of a centrally sectioned guinea pig knee joint based upon Cashin35 and reproduced by permission of Oxford University Press. (b) A series of four sections taken at 200μm intervals in the coronal plane, spanning the anterior of the joint through to the center. Note the elongated appearance of the lateral meniscus (on left) and the more rounded appearance of the medial meniscus (on right) when the center of the knee joint is attained. Osteoarthritis and Cartilage 2010 18, S35-S52DOI: (10.1016/j.joca.2010.04.015) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Plane of sectioning of the guinea pig knee joint. The lateral aspect of the guinea pig knee showing the plane of sectioning (A–A′) based on Cashin35 and reproduced by permission of Oxford University Press. Note that the knee is in slight flexion. Osteoarthritis and Cartilage 2010 18, S35-S52DOI: (10.1016/j.joca.2010.04.015) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Zones of articular cartilage. Accurate scoring requires the ability to distinguish the four zones of articular cartilage as depicted here: I-superficial zone, II-middle zone, III-deep zone, and IV-calcified cartilage zone, and the tidemark separating zones III and IV. Reproduced by permission of Dr. James Williams. Osteoarthritis and Cartilage 2010 18, S35-S52DOI: (10.1016/j.joca.2010.04.015) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 Representative images of guinea pig knees (aged 2–18 months) demonstrating varying severities of histological lesions. Examples of lesions scored by the recommended histochemical-histological scoring scheme. The mean score generated by two experts is shown in the bottom right of each panel in Fig. 7(a)–(c). Cartilage structure (CS) scoring atlas. Microscopic toluidine blue stained images of the guinea pig knee joint demonstrating the nine levels (scores 0–8) of cartilage structure (CS) corresponding to the criteria in Table IV. Magnification bar 500μm. (b) Proteoglycan content (PG) and Tidemark (TM) scoring atlas. Microscopic toluidine blue stained images of the guinea pig knee joint demonstrating the seven levels (scores 0–6) of proteoglycan content (PG) (Magnification bar=500μm), and the two levels (scores 0–1) of Tidemark (TM) integrity (Magnification bar=50μm) corresponding to the criteria in Table IV. (c) Osteophyte (OST) and Cellularity (CL) scoring atlas. Microscopic toluidine blue stained images of the guinea pig knee joint demonstrating the four levels (scores 0–3) of osteophyte (OST) (Magnification bar=250μm), and the four levels (scores 0–3) of Cellularity (CL) (Magnification bar=50μm) corresponding to the criteria in Table IV. Osteoarthritis and Cartilage 2010 18, S35-S52DOI: (10.1016/j.joca.2010.04.015) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 Representative images of guinea pig knees (aged 2–18 months) demonstrating varying severities of histological lesions. Examples of lesions scored by the recommended histochemical-histological scoring scheme. The mean score generated by two experts is shown in the bottom right of each panel in Fig. 7(a)–(c). Cartilage structure (CS) scoring atlas. Microscopic toluidine blue stained images of the guinea pig knee joint demonstrating the nine levels (scores 0–8) of cartilage structure (CS) corresponding to the criteria in Table IV. Magnification bar 500μm. (b) Proteoglycan content (PG) and Tidemark (TM) scoring atlas. Microscopic toluidine blue stained images of the guinea pig knee joint demonstrating the seven levels (scores 0–6) of proteoglycan content (PG) (Magnification bar=500μm), and the two levels (scores 0–1) of Tidemark (TM) integrity (Magnification bar=50μm) corresponding to the criteria in Table IV. (c) Osteophyte (OST) and Cellularity (CL) scoring atlas. Microscopic toluidine blue stained images of the guinea pig knee joint demonstrating the four levels (scores 0–3) of osteophyte (OST) (Magnification bar=250μm), and the four levels (scores 0–3) of Cellularity (CL) (Magnification bar=50μm) corresponding to the criteria in Table IV. Osteoarthritis and Cartilage 2010 18, S35-S52DOI: (10.1016/j.joca.2010.04.015) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 8 Examples of synovitis associated with spontaneous OA in the Hartley guinea pig knee joint. Representative images (100×) of synovitis scored according to the recommended scoring system in Table VI. Sections stained with toluidine blue (a and c) or Hematoxylin and Eosin (b and d). Normal synovium from a 60-day-old animal (a); Synovitis scores of three in a 145-day-old animal (b) and a 180-day-old animal (c) based on scoring synovial hyperplasia=1 (3–5 layers), villous hyperplasia=1, and scattered infiltrate=1. Synovitis score of seven in a 2-year-old animal (d) based on scoring synovial hyperplasia=2 (≥6 layers), villous hyperplasia=3, and infiltrate=2. Osteoarthritis and Cartilage 2010 18, S35-S52DOI: (10.1016/j.joca.2010.04.015) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 9 Effect of caging on guinea pig knee joint histological OA severity. Animals were housed either singly (n=26, white bars), or in pairs (n=14, dark bars) from 2 to 12 months of age. Paraffin central sections of these knee joints (aged 12 months) were scored using the recommended scoring scheme to assess cartilage structure (CS) and proteoglycan content (PG) or a combination of both (Total), of the medial tibia (MT) or entire medial compartment (MED). A comparison of paired housing with single housing by the non-parametric Mann–Whitney test, was associated with slight but significantly greater histological scores in the paired-housed animals compared with single-housed animals (*P=0.02; §P=0.04, respectively). Error bars=SEM. Osteoarthritis and Cartilage 2010 18, S35-S52DOI: (10.1016/j.joca.2010.04.015) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions