Microtubule-based movement of PxdA colocalizes with moving peroxisomes

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Microtubule-based movement of PxdA colocalizes with moving peroxisomes. Microtubule-based movement of PxdA colocalizes with moving peroxisomes. (A) Representative kymograph generated from a movie of PxdA-GFP. (B) Histogram of velocities calculated from PxdA-GFP and PxdA-mKate kymographs. Mean velocity is 2.32 ± 0.88 (SD; n = 328). (C) Bar graph of the number of PxdA-mKate movements calculated as the number of puncta crossing a line drawn perpendicular to the hyphal long axis during a 10-s movie. PxdA puncta moved 5.48 ± 0.62 (SEM) per hyphae in the DMSO control (C; n = 21 hyphae), 0.33 ± 0.16 per hyphae in the presence of benomyl (Ben; n = 15), and 4.81 ± 0.57 per hyphae in the presence of latrunculin A (Lat A; n = 16). Benomyl but not latrunculin A treatment was significantly different than the control (one-way analysis of variance, Bonferroni post hoc test, *, P < 0.001). (D) Representative kymographs generated from simultaneous time-lapse movies of peroxisomes (Pex) and PxdA-GFP. 65.6% (n = 29) of moving peroxisomes overlapped with PxdA. Bars: (x axis) 5 µm; (y axis) 5 s. Left panels correspond to Video 5. (E) Stills from Video 6 of peroxisomes (Pex) and PxdA-GFP. White arrows point to a co-migrating PxdA puncta and peroxisome. Bar, 5 µm. (F) Normalized intensity line scans of peroxisomes and PxdA during comigrating runs (n = 11). John Salogiannis et al. J Cell Biol 2016;212:289-296 © 2016 Salogiannis et al.