Confocal images of negative controls for Aβ42, Synaptophysin (syp), and PSD95 in hippocampal neurons. Confocal images of negative controls for Aβ42, Synaptophysin.

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Confocal images of negative controls for Aβ42, Synaptophysin (syp), and PSD95 in hippocampal neurons. Confocal images of negative controls for Aβ42, Synaptophysin (syp), and PSD95 in hippocampal neurons. (A) Hippocampal neurons stained with Aβ42 (red) and synaptophysin (green), using the same primary and secondary antibodies as for STED images in Fig 3A and B combined with actin (light grey) staining and imaged by confocal microscopy. Scale bars: 5 μm. (B) Negative control for synaptophysin (syp neg cntr). Hippocampal neurons only stained with Aβ42 (red) and the same secondary antibodies (Abberior STAR 635P and Alexa Fluor 594) as for STED images in Fig 3A and B combined with actin (light grey) staining and imaged by confocal microscopy in the same setting as in (A). Scale bars: 5 μm. (C) Hippocampal neurons stained for Aβ42 (red) and PSD95 (cyan), using the same primary and secondary antibodies as for STED in Fig 3C and D combined with actin (light grey) staining and imaged by confocal microscopy. Scale bars: 5 μm. (D) Negative control for PSD95 (PSD95 neg cntr). Hippocampal neurons only stained for Aβ42 (red), using the same secondary antibodies (Abberrior STAR 635P and Alexa Fluor 594) as for STED images in Fig 3C and D combined with actin (light grey) staining and imaged by confocal microscopy in the same setting as in (C). Scale bars: 5 μm. (E) Negative control for Aβ42 (Aβ42 neg cntr). Hippocampal neurons only stained for PSD95 (cyan), using the same secondary antibodies (Abberrior STAR 635P and Alexa Fluor 594) as for STED in Fig 3C and D combined with actin (light grey) staining and imaged by confocal microscopy in the same setting as in (C). Scale bars: 5 μm. Yang Yu et al. LSA 2018;1:e201800028 © 2018 Yu et al.