Development of a real-time PCR method for rapid sexing of human preimplantation embryos CD Martinhago, LD Vagnini, CG Petersen, AL Mauri, RL Baruffi,

Slides:

Advertisements

Similar presentations

Development of guideline-based quality indicators for recurrent miscarriage E. van den Boogaard, M. Goddijn, N.J. Leschot, F. van der Veen, J.A.M. Kremer,

Advertisements

Slow freezing, vitrification and ultra-rapid freezing of human embryos: a systematic review and meta-analysis Faten F. AbdelHafez, Nina Desai, Ahmed.

Programmes and prospects for ovotechnology

Attitudes and policies regarding access to fertility care and assisted reproductive technologies in Israel Daniel Sperling, Yael Simon Reproductive.

Diet and endometriosis risk: A literature review

Hatem Abu Hashim Reproductive BioMedicine Online

Delphine Haouzi, Hervé Dechaud, Said Assou, John De Vos, Samir Hamamah

Relationship between DNA damage and sperm head birefringence

E. Jauniaux, I. Ben-Ami, R. Maymon Reproductive BioMedicine Online

E. Jauniaux, I. Ben-Ami, R. Maymon Reproductive BioMedicine Online

MiR-483–5p and miR-486–5p are down-regulated in cumulus cells of metaphase II oocytes from women with polycystic ovary syndrome Lin Shi, Shan Liu, Wanqiu.

Willem Ombelet Reproductive BioMedicine Online

The effect of endometrial scratch on natural-cycle cryopreserved embryo transfer outcomes: a randomized controlled study Jennifer Sze Man Mak, Cathy.

First successful application of preimplantation genetic diagnosis and haplotyping for congenital hyperinsulinism Wafa Qubbaj, Abdulrahman Al-Swaid, Saad.

Fresh transfer outcome predicts the success of a subsequent frozen transfer utilizing blastocysts of the same cohort L.F. Doherty, J.R. Martin, U. Kayisli,

Semra Kahraman, Zafer Nihat Candan Reproductive BioMedicine Online

Why are reproductive cancers more common in nulliparous women?

Perinatal outcomes after stimulated versus natural cycle IVF: a systematic review and meta-analysis Mohan Shashikant Kamath, Richard Kirubakaran, Mariano.

K. Gersak, A. Veble Reproductive BioMedicine Online

Désirée García, Rita Vassena, Andrés Prat, Valérie Vernaeve

Kiran Singh, Rohini R. Nair, Anuradha Khanna

S Munné, E Fragouli, P Colls, MG Katz-Jaffe, WB Schoolcraft, D Wells

Pregnancy and live birth following the transfer of vitrified–warmed blastocysts derived from zona- and corona-cell-free oocytes Yimin Shu, Wenlin Peng,

A Girardet, C Fernandez, M Claustres Reproductive BioMedicine Online

Working to eliminate multiple pregnancies: a success story in Québec

Sarah McCredie, William Ledger, Christos A. Venetis

Effect of N-acetyl-cysteine after ovarian drilling in clomiphene citrate-resistant PCOS women: a pilot study A. Nasr Reproductive BioMedicine Online

L. Simon, I. Proutski, M. Stevenson, D. Jennings, J. McManus, D

Cumulative live-birth delivery after IVF/ICSI since the progressive introduction of single- embryo transfer D. De Neubourg, C. Daels, M. Elseviers, K.

Endometrial injury to overcome recurrent embryo implantation failure: a systematic review and meta-analysis Neelam Potdar, Tarek Gelbaya, Luciano G.

Effect of energy expenditure and physical activity on the outcomes of assisted reproduction treatment Mert Kucuk, Fadime Doymaz, Bulent Urman Reproductive.

Morphological systems of human embryo assessment and clinical evidence

Cross-border reproductive care: a review of the literature

Lisa Cummins, Juliette Koch, Suha Kilani

The oviduct: a neglected organ due for re-assessment in IVF

Association between leukaemia inhibitory factor gene polymorphism and pregnancy outcomes after assisted reproduction techniques Joao Batista A. Oliveira,

Intrafollicular soluble receptor for advanced glycation end products (sRAGE) and embryo quality in assisted reproduction Tatiana C.S. Bonetti, Edson.

Fernando Zegers-Hochschild, Juan Enrique Schwarze, Javier A

Alexis Adler, Hsaio-Ling Lee, David H

V Isachenko, E Isachenko, R Kreienberg, M Woriedh, JM Weiss

P Colls, N Goodall, X Zheng, S Munné Reproductive BioMedicine Online

Intra-cycle variation of the uterine cavity indentation assessed with three-dimensional ultrasound in natural and stimulated cycles Sotirios H. Saravelos,

LH concentrations do not correlate with pregnancy in rFSH/GnRH antagonist cycles Kevin Doody, Paul Devroey, Keith Gordon, Han Witjes, Bernadette Mannaerts

Mohamed A. Aboulghar, Heba Marie, Yahia M. Amin, Mona M

Stanley Paul Leibo (1937–2014): scientist, mentor and friend

Morphokinetic parameters in chromosomal translocation carriers undergoing preimplantation genetic testing Jenna Lammers, Arnaud Reignier, Carole Splingart,

Follicle recruitment determines IVF productivity rate via the number of embryos frozen and subsequent transfers James D. Stanger, John L. Yovich Reproductive.

Should we be promoting embryo transfer at blastocyst stage?

Stephen C. Collins, Esther Chan Reproductive BioMedicine Online

Pietro Gambadauro, Ramesan Navaratnarajah

Implication of apoptosis in sperm cryoinjury

Samuel Kofi Arhin, Yu Zhao, XiaoSheng Lu, Mandika Chetry, JieQiang Lu

Tetsuya Ishii Reproductive BioMedicine Online

The Alpha Consensus Meeting on the professional status of the clinical embryologist: proceedings of an expert meeting Reproductive BioMedicine Online

C Almeida, M Sousa, A Barros Reproductive BioMedicine Online

Effect of piroxicam administration before embryo transfer on IVF outcome: a randomized controlled trial Luca Dal Prato, Andrea Borini Reproductive BioMedicine.

Elective single-embryo transfer: persuasive communication strategies can affect choice in a young British population O.B.A. van den Akker, S. Purewal

Management of endometrial polyps diagnosed before or during ICSI cycles Bulent Tiras, Umit Korucuoglu, Mehtap Polat, Hulusi Bulent Zeyneloglu, Ayse Saltik,

Ovarian reserve screening before contraception?

Live birth following early follicular phase oocyte collection and vitrified-warmed embryo transfer 8 days later Safak Hatırnaz, Ebru Hatırnaz, Baris.

The nature and origin of binucleate cells in human preimplantation embryos: relevance to placental mesenchymal dysplasia L. Xanthopoulou, J.D.A. Delhanty,

Implantation of fresh and thawed–warmed embryos in single embryo transfer cycles: interpreting the initial beta-HCG Cynthia K. Sites, Peter St Marie,

Programmes and prospects for ovotechnology

Claudio Manna, Loris Nanni, Alessandra Lumini, Sebastiana Pappalardo

A. G. Sutcliffe, J. T. Manning, A. Katalanic, A. Ludwig, M. Mehta, J

Randomized controlled trial of benzocaine versus placebo spray for pain relief at hysterosalpingogram E.A. Bachman, S. Senapati, M.D. Sammel, S.K. Kalra

China’s model of egg donation is a policy lesson for Britain

A. Finn, L. Scott, Thomas O’Leary, D. Davies, J. Hill

Preimplantation genetic screening: results of a worldwide web-based survey Ariel Weissman, Gon Shoham, Zeev Shoham, Simon Fishel, Milton Leong, Yuval.

Yakoub Khalaf, Susan Bewley, Peter Braude

Presentation transcript:

Development of a real-time PCR method for rapid sexing of human preimplantation embryos CD Martinhago, LD Vagnini, CG Petersen, AL Mauri, RL Baruffi, RM de Oliveira, JG Franco Reproductive BioMedicine Online Volume 20, Issue 1, Pages 75-82 (January 2010) DOI: 10.1016/j.rbmo.2009.10.008 Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure. 1 Scatter diagrams of threshold cycle (Ct) and the baseline-corrected normalized fluorescence (ΔRn) from single cells (buccal cell and blastomere). Comparison of TSPY and β-globin signals on real-time polymerase chain reaction reaction with 25μl (A), and 50μl (B). Reproductive BioMedicine Online 2010 20, 75-82DOI: (10.1016/j.rbmo.2009.10.008) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions