J.A. Collins, R.J. Moots, R. Winstanley, P.D. Clegg, P.I. Milner 

Slides:



Advertisements
Similar presentations
Soluble factors from the notochordal-rich intervertebral disc inhibit endothelial cell invasion and vessel formation in the presence and absence of pro-inflammatory.
Advertisements

Efficacy of ultrasound therapy for the management of knee osteoarthritis: a systematic review with meta-analysis A. Loyola-Sánchez, J. Richardson, N.J.
M. M. J. Caron, P. J. Emans, M. M. E. Coolsen, L. Voss, D. A. M
B. Bai, Y. Li  Osteoarthritis and Cartilage 
The disease modifying osteoarthritis drug diacerein is able to antagonize pro inflammatory state of chondrocytes under mild mechanical stimuli  B. Steinecker-Frohnwieser,
A potential role of chondroitin sulfate on bone in osteoarthritis: inhibition of prostaglandin E2 and matrix metalloproteinases synthesis in interleukin-1β-
BMP-2 induces the expression of chondrocyte-specific genes in bovine synovium- derived progenitor cells cultured in three-dimensional alginate hydrogel 
IL-10 reduces apoptosis and extracellular matrix degradation after injurious compression of mature articular cartilage  P. Behrendt, A. Preusse-Prange,
Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression  T. Piepoli,
Combination of ADMSCs and chondrocytes reduces hypertrophy and improves the functional properties of osteoarthritic cartilage  M.R. Ahmed, A. Mehmood,
Characterization of diabetic osteoarthritic cartilage and role of high glucose environment on chondrocyte activation: toward pathophysiological delineation.
M. M. J. Caron, P. J. Emans, M. M. E. Coolsen, L. Voss, D. A. M
Chondroitin sulphate: an effective joint lubricant?
Cannabinoid WIN-55,212-2 mesylate inhibits interleukin-1β induced matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase expression.
Stress-induced signaling pathways in hyalin chondrocytes: inhibition by Avocado– Soybean Unsaponifiables (ASU)  O. Gabay, B.Sc., Ph.D. fellow, M. Gosset,
J.E. Lafont, F.-A. Poujade, M. Pasdeloup, P. Neyret, F. Mallein-Gerin 
K.A. Payne, D.M. Didiano, C.R. Chu  Osteoarthritis and Cartilage 
Global analysis of nuclear receptor expression and dysregulation in human osteoarthritic articular cartilage  L.A. Collins-Racie, Z. Yang, M. Arai, N.
Mitochondrial function is altered in articular chondrocytes of an endemic osteoarthritis, Kashin–Beck disease  J.T. Liu, X. Guo, W.J. Ma, Y.G. Zhang,
C. M. Keenan, A. Beckett, H. Sutherland, I. Prior, L. R. Ranganath, J
Aging-related inflammation in osteoarthritis
Synergistic effect of IGF-1 and OP-1 on matrix formation by normal and OA chondrocytes cultured in alginate beads  S. Chubinskaya, Ph.D., A. Hakimiyan,
Assessment of strategies to increase chondrocyte viability in cryopreserved human osteochondral allografts: evaluation of the glycosylated hydroquinone,
Roles for the interleukin-4 receptor and associated JAK/STAT proteins in human articular chondrocyte mechanotransduction  S.J. Millward-Sadler, Ph.D.,
Osmolarity effects on bovine articular chondrocytes during three-dimensional culture in alginate beads  X. Xu, J.P.G. Urban, U.K. Tirlapur, Z. Cui  Osteoarthritis.
Cytochrome c oxidase levels in chondrocytes during monolayer expansion and after return to three dimensional culture  O.A. Boubriak, J.T.S. Brooks, J.P.G.
Osteoarthritis year 2011 in review: biochemical markers of osteoarthritis: an overview of research and initiatives  Y. Henrotin  Osteoarthritis and Cartilage 
Expression and regulation of Toll-like receptor 2 by IL-1β and fibronectin fragments in human articular chondrocytes  S.-L. Su, M.S., C.-D. Tsai, Ph.D.,
Decreased proteoglycan degradation in IL-1β-treated cartilage co-cultured with TIMP-3- transduced cells  J. Mason, A. Donahue, A. Yoskowitz, D. Richardson 
Osteoblasts from the sclerotic subchondral bone downregulate aggrecan but upregulate metalloproteinases expression by chondrocytes. This effect is mimicked.
Glucosamine reduces anabolic as well as catabolic processes in bovine chondrocytes cultured in alginate  E.J. Uitterlinden, M.D., H. Jahr, Ph.D., J.L.M.
M.A. Greene, R.F. Loeser  Osteoarthritis and Cartilage 
H Jo, M. D. , J. S Park, Ph. D. , E. M Kim, M. S. , M. Y Jung, Ph. D
Salubrinal reduces expression and activity of MMP13 in chondrocytes
Osteogenic protein-1 promotes the formation of tissue-engineered cartilage using the alginate-recovered-chondrocyte method  Dr. K. Masuda, M.D., B.E.
PCB126 induces apoptosis of chondrocytes via ROS-dependent pathways
Methylation of the OP-1 promoter: potential role in the age-related decline in OP-1 expression in cartilage  R.F. Loeser, M.D., H.-J. Im, Ph.D., B. Richardson,
M. M. J. Caron, P. J. Emans, M. M. E. Coolsen, L. Voss, D. A. M
Stem cell therapy for human cartilage defects: a systematic review
Rapid in situ chondrocyte death induced by Staphylococcus aureus toxins in a bovine cartilage explant model of septic arthritis  I.D.M. Smith, J.P. Winstanley,
T. Kurth, M. Sc. , E. Hedbom, Ph. D. , N. Shintani, Ph. D. , M
Reactive nitrogen and oxygen species in interleukin-1-mediated DNA damage associated with osteoarthritis  C.M. Davies, Ph.D., F. Guilak, Ph.D., J.B. Weinberg,
Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression  T. Piepoli,
Hypoxia differentially regulates human nucleus pulposus and annulus fibrosus cell extracellular matrix production in 3D scaffolds  G. Feng, L. Li, H.
Different responses of healthy, relative healthy and OA chondrocytes with IL1β treatment under hypoxia and normoxia  X. Huang, L. Zhong, J. Post, M. Karperien 
N. Gómez-Prieto, Á. Vela-Anero, L. Gato-Calvo, C. Ruiz-Romero, R
P. I. Milner, Ph. D. , B. V. Sc. , B. Sc. (Hons. ), M. R. C. V. S. , R
J. A. Martin, A. Martini, A. Molinari, W. Morgan, W. Ramalingam, J. A
Who should have a joint replacement? A plea for more ‘phronesis’
Synergistic effect of chondroitin sulfate and cyclic pressure on biochemical and morphological properties of chondrocytes from articular cartilage  G.
Safety of intra-articular cell-therapy with culture-expanded stem cells in humans: a systematic literature review  C.M.M. Peeters, M.J.C. Leijs, M. Reijman,
Effect of a glucosamine derivative on impact-induced chondrocyte apoptosis in vitro. A preliminary report  C.A.M. Huser, Ph.D., M.E. Davies, Ph.D.  Osteoarthritis.
Increased susceptibility to IL-1 accelerates osteoarthritic change in 3D-cultured chondrocytes and in vivo animal models  Y. Takeda, Y. Niki, Y. Fukuhara,
Chondroitin sulfate modulation of matrix and inflammatory gene expression in IL-1β- stimulated chondrocytes – study in hypoxic alginate bead cultures 
Differential regulation of proteoglycan 4 metabolism in cartilage by IL-1α, IGF-I, and TGF-β1  T.A. Schmidt, Ph.D., N.S. Gastelum, B.S., E.H. Han, M.S.,
Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis  D.H. Rosenzweig,
Erratum to “MMP and non-MMP-mediated release of aggrecan and its fragments from articular cartilage: a comparative study of three different aggrecan and.
D.J. Hunter  Osteoarthritis and Cartilage 
Therapeutic trajectory following intra-articular hyaluronic acid injection in knee osteoarthritis – meta-analysis  R.R. Bannuru, N.S. Natov, U.R. Dasi,
Estrogen reduces mechanical injury-related cell death and proteoglycan degradation in mature articular cartilage independent of the presence of the superficial.
Osteoarthritis year 2012 in review: biology
Systematic review of the impact of osteoarthritis on health outcomes for comorbid disease in older people  L. Parkinson, D.L. Waters, L. Franck  Osteoarthritis.
Hydrogen sulfide donors alleviate IL-1β induced inflammation-like effects in human articular osteoarthritic chondrocytes  E.F. Burguera, A. Vela-Anero,
Erratum to “Effect of hyaluronic acid (MW 500–730kDa) on proteoglycan and nitric oxide production in human osteoarthritic chondrocyte cultures exposed.
Inhibition of adenosine kinase attenuates interleukin-1- and lipopolysaccharide-induced alterations in articular cartilage metabolism  Raina Petrov, B.S.,
Peroxisome proliferator activated receptor alpha activation decreases inflammatory and destructive responses in osteoarthritic cartilage  S. Clockaerts,
Osteoarthritis year in review 2016: mechanics
M. Doherty, P. Dieppe  Osteoarthritis and Cartilage 
Structured three-dimensional co-culture of mesenchymal stem cells with chondrocytes promotes chondrogenic differentiation without hypertrophy  M.E. Cooke,
General Information Osteoarthritis and Cartilage
Presentation transcript:

Oxygen and pH-sensitivity of human osteoarthritic chondrocytes in 3-D alginate bead culture system  J.A. Collins, R.J. Moots, R. Winstanley, P.D. Clegg, P.I. Milner  Osteoarthritis and Cartilage  Volume 21, Issue 11, Pages 1790-1798 (November 2013) DOI: 10.1016/j.joca.2013.06.028 Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Effect of oxygen tension and pH on cell viability of human osteoarthritic articular chondrocytes over 96 h. Cell viability was determined by the trypan blue exclusion and presented as mean values compared to control (time = 0, 5% O2, pH 7.2). Osteoarthritic chondrocytes were cultured in three-dimensional alginate beads in <1% (anoxia), 2% (hypoxia), 5% (normoxia for chondrocytes) or 21% O2 (hyperoxia for chondrocytes) at pH 7.2 (normal pH) (A) or 6.2 (acidosis) (B) and cell viability was measured after 24, 48 h and 96 h. Line graphs represent means ± 95% CI, n = 3 donors. *P < 0.05; †P < 0.01. Osteoarthritis and Cartilage 2013 21, 1790-1798DOI: (10.1016/j.joca.2013.06.028) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Effect of oxygen tension and pH on GAG release from human osteoarthritic articular chondrocytes over 96 h. GAG release was determined by the DMMB assay and presented as mean values compared to control (time = 0, 5% O2, pH 7.2). Osteoarthritic chondrocytes were cultured in three-dimensional alginate beads in <1% (anoxia), 2% (hypoxia), 5% (normoxia for chondrocytes) or 21% O2 (hyperoxia for chondrocytes) at pH 7.2 (normal pH) (A) or 6.2 (acidosis) (B) and GAG release into culture media was measured spent media after 24, 48 h and 96 h. Line graphs represent means ± 95% CI, n = 3 donors. *P < 0.05; †P < 0.01. Osteoarthritis and Cartilage 2013 21, 1790-1798DOI: (10.1016/j.joca.2013.06.028) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Effect of oxygen tension and pH on ATPi in human osteoarthritic articular chondrocytes over 96 h. Osteoarthritic chondrocytes were cultured in three-dimensional alginate beads in <1% (anoxia), 2% (hypoxia), 5% (normoxia for chondrocytes) or 21% O2 (hyperoxia for chondrocytes) at pH 7.2 (normal pH) (A) or 6.2 (acidosis) (B) and ATPi was determined after 24, 48 h and 96 h using the EnzyLight™ ATP assay kit. Line graphs represent means ± 95% CI, n = 3 donors. *P < 0.05; †P < 0.01. Osteoarthritis and Cartilage 2013 21, 1790-1798DOI: (10.1016/j.joca.2013.06.028) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Effect of oxygen tension, pH and IL-1β on ROS levels from human osteoarthritic articular chondrocytes. ROS were measured as DCF fluorescence and presented as mean values compared to control (time = 0, 5% O2, pH 7.2). Osteoarthritic chondrocytes were cultured in three-dimensional alginate beads in <1% (anoxia), 2% (hypoxia), 5% (normoxia for chondrocytes) or 21% (hyperoxia for chondrocytes) O2 for 48 h at pH 7.2 (normal pH) or 6.2 (acidosis) in the absence or presence of IL-1β (10 ng/ml). Bar chart represents mean and 95% CI, n = 3 donors. *P < 0.05. Osteoarthritis and Cartilage 2013 21, 1790-1798DOI: (10.1016/j.joca.2013.06.028) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Effect of oxygen tension, pH and IL-1β on the mitochondrial membrane potential of human osteoarthritic articular chondrocytes. The mitochondrial membrane potential was measured using the fluorescent probe JC-1 and results are presented as mean values compared to control (time = 0, 5% O2, pH 7.2). Osteoarthritic chondrocytes were cultured in three-dimensional alginate beads in <1% (anoxia), 2% (hypoxia), 5% (normoxia for chondrocytes) or 21% (hyperoxia for chondrocytes) O2 for 48 h at pH 7.2 (normal pH) or 6.2 (acidosis) in the absence or presence of IL-1β (10 ng/ml). Bar chart represents mean and 95% CI, n = 3 donors. †P < 0.01. Osteoarthritis and Cartilage 2013 21, 1790-1798DOI: (10.1016/j.joca.2013.06.028) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Effect of oxygen tension, pH and IL-1β on the GSH:GSSG ratio of human osteoarthritic articular chondrocytes. The GSH:GSSG ratio was measured using the luminescence based GSH/GSSG-Glo™ Assay and results are presented as mean values compared to control (time = 0, 5% O2, pH 7.2). Osteoarthritic chondrocytes were cultured in three-dimensional alginate beads in <1% (anoxia), 2% (hypoxia), 5% (normoxia for chondrocytes) or 21% (hyperoxia for chondrocytes) O2 for 48 h at pH 7.2 (normal pH) or 6.2 (acidosis) in the absence or presence of IL-1β (10 ng/ml). Bar chart represents mean ± 95% CI, n = 3 donors. *P < 0.05; †P < 0.01. Osteoarthritis and Cartilage 2013 21, 1790-1798DOI: (10.1016/j.joca.2013.06.028) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions