Quantification of readthrough peptides in the presence of paromomycin Quantification of readthrough peptides in the presence of paromomycin. a–c) Mass spectrometry extracted-ion chromatograms of readthrough peptides for UAA, UAG and UGA stop codons. d) Relative frequencies of the readthrough amino acids incorporated at UAA, U... Quantification of readthrough peptides in the presence of paromomycin. a–c) Mass spectrometry extracted-ion chromatograms of readthrough peptides for UAA, UAG and UGA stop codons. d) Relative frequencies of the readthrough amino acids incorporated at UAA, UAG and UGA in the presence (+) and absence (−) of paromomycin. Quantifications were performed as described in the Materials and methods section. #: data previously obtained by Blanchet et al. [16] in the same conditions. Iwona Pranke et al. ERJ Open Res 2018;4:00080-2017 ©2018 by European Respiratory Society