Kim Min Jung , Ciani Silvano , Schachtman Daniel P.   Molecular Plant 

Slides:



Advertisements
Similar presentations
Potassium Transporter KUP7 Is Involved in K+ Acquisition and Translocation in Arabidopsis Root under K+-Limited Conditions  Min Han, Wei Wu, Wei-Hua Wu,
Advertisements

Figure S1 A B C D E F G Long Day Hypocotyl lenght (mm)
A Histone H3 Lysine-27 Methyltransferase Complex Represses Lateral Root Formation in Arabidopsis thaliana  Gu Xiaofeng , Xu Tongda , He Yuehui   Molecular.
Volume 7, Issue 6, Pages (June 2014)
Volume 8, Issue 3, Pages (March 2015)
Zhu Hui-Fen , Fitzsimmons Karen , Khandelwal Abha , Kranz Robert G.  
Volume 23, Issue 18, Pages (September 2013)
Volume 7, Issue 9, Pages (September 2014)
Roger B. Deal, Steven Henikoff  Developmental Cell 
Volume 4, Issue 1, Pages (January 2011)
Volume 25, Issue 19, Pages (October 2015)
Spatiotemporal Brassinosteroid Signaling and Antagonism with Auxin Pattern Stem Cell Dynamics in Arabidopsis Roots  Juthamas Chaiwanon, Zhi-Yong Wang 
Jun-Ho Ha, Hyo-Jun Lee, Jae-Hoon Jung, Chung-Mo Park 
Volume 2, Issue 1, Pages (January 2009)
Volume 5, Issue 2, Pages (March 2012)
Volume 7, Issue 5, Pages (May 2014)
Volume 10, Issue 11, Pages (November 2017)
Luo Chongyuan , Durgin Brittany G. , Watanabe Naohide , Lam Eric  
Potassium Transporter KUP7 Is Involved in K+ Acquisition and Translocation in Arabidopsis Root under K+-Limited Conditions  Min Han, Wei Wu, Wei-Hua Wu,
A Truncated Arabidopsis NUCLEOSOME ASSEMBLY PROTEIN 1, AtNAP1;3T, Alters Plant Growth Responses to Abscisic Acid and Salt in the Atnap1;3-2 Mutant  Liu.
Takatoshi Kiba, Kentaro Takei, Mikiko Kojima, Hitoshi Sakakibara 
Volume 10, Issue 6, Pages (June 2017)
Volume 8, Issue 3, Pages (March 2015)
Volume 8, Issue 5, Pages (May 2015)
Volume 21, Issue 12, Pages (December 2017)
Arabidopsis ROP1 and ROP6 Influence Germination Time, Root Morphology, the Formation of F-Actin Bundles, and Symbiotic Fungal Interactions  Yvonne Venus,
Volume 7, Issue 9, Pages (September 2014)
Volume 6, Issue 6, Pages (November 2013)
A DTX/MATE-Type Transporter Facilitates Abscisic Acid Efflux and Modulates ABA Sensitivity and Drought Tolerance in Arabidopsis  Haiwen Zhang, Huifen.
The WUSCHEL Related Homeobox Protein WOX7 Regulates the Sugar Response of Lateral Root Development in Arabidopsis thaliana  Danyu Kong, Yueling Hao, Hongchang.
Rodríguez-Milla Miguel A. , Salinas Julio   Molecular Plant 
Volume 19, Issue 15, Pages (August 2009)
Volume 18, Issue 10, Pages (May 2008)
Jaimie M. Van Norman, Rebecca L. Frederick, Leslie E. Sieburth 
Volume 66, Issue 5, Pages e4 (June 2017)
Volume 5, Issue 3, Pages (May 2012)
Volume 5, Issue 4, Pages (July 2012)
The Arabidopsis Transcription Factor AtTCP15 Regulates Endoreduplication by Modulating Expression of Key Cell-cycle Genes  Li Zi-Yu , Li Bin , Dong Ai-Wu.
Volume 3, Issue 3, Pages (May 2010)
Arabidopsis MSBP1 Is Activated by HY5 and HYH and Is Involved in Photomorphogenesis and Brassinosteroid Sensitivity Regulation  Shi Qiu-Ming , Yang Xi.
Volume 3, Issue 3, Pages (March 2013)
AtABCG29 Is a Monolignol Transporter Involved in Lignin Biosynthesis
Volume 5, Issue 5, Pages (September 2012)
Volume 4, Issue 2, Pages (March 2011)
Arabidopsis WRKY45 Interacts with the DELLA Protein RGL1 to Positively Regulate Age-Triggered Leaf Senescence  Ligang Chen, Shengyuan Xiang, Yanli Chen,
Arabidopsis NF-YCs Mediate the Light-Controlled Hypocotyl Elongation via Modulating Histone Acetylation  Yang Tang, Xuncheng Liu, Xu Liu, Yuge Li, Keqiang.
HOS1 Facilitates the Phytochrome B-Mediated Inhibition of PIF4 Function during Hypocotyl Growth in Arabidopsis  Ju-Heon Kim, Hyo-Jun Lee, Jae-Hoon Jung,
A ROP GTPase Signaling Pathway Controls Cortical Microtubule Ordering and Cell Expansion in Arabidopsis  Ying Fu, Tongda Xu, Lei Zhu, Mingzhang Wen, Zhenbiao.
BZR1 Interacts with HY5 to Mediate Brassinosteroid- and Light-Regulated Cotyledon Opening in Arabidopsis in Darkness  Qian-Feng Li, Jun-Xian He  Molecular.
Volume 9, Issue 8, Pages (August 2016)
Volume 5, Issue 6, Pages (November 2012)
Volume 7, Issue 8, Pages (August 2014)
Volume 4, Issue 4, Pages (July 2011)
Dissection of miRNA Pathways Using Arabidopsis Mesophyll Protoplasts
1O2-Mediated and EXECUTER-Dependent Retrograde Plastid-to-Nucleus Signaling in Norflurazon-Treated Seedlings of Arabidopsis thaliana  Chanhong Kim, Klaus.
Volume 4, Issue 5, Pages (September 2011)
Volume 2, Issue 1, Pages (January 2009)
Mitochondrial Perturbation Negatively Affects Auxin Signaling
Volume 25, Issue 7, Pages e4 (November 2018)
Volume 7, Issue 12, Pages (December 2014)
Volume 6, Issue 2, Pages (March 2013)
Arabidopsis ROP1 and ROP6 Influence Germination Time, Root Morphology, the Formation of F-Actin Bundles, and Symbiotic Fungal Interactions  Yvonne Venus,
Volume 10, Issue 4, Pages (April 2017)
Volume 1, Issue 1, Pages (January 2008)
Volume 7, Issue 7, Pages (July 2014)
Mitochondrial Sulfide Detoxification Requires a Functional Isoform O- Acetylserine(thiol)lyase C in Arabidopsis thaliana  Consolación Álvarez, Irene García,
Volume 1, Issue 1, Pages 4-14 (January 2008)
Volume 27, Issue 5, Pages (March 2017)
Volume 11, Issue 7, Pages (July 2018)
Volume 5, Issue 3, Pages (May 2012)
Presentation transcript:

A Peroxidase Contributes to ROS Production during Arabidopsis Root Response to Potassium Deficiency  Kim Min Jung , Ciani Silvano , Schachtman Daniel P.   Molecular Plant  Volume 3, Issue 2, Pages 420-427 (March 2010) DOI: 10.1093/mp/ssp121 Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 1 Overexpression of the RCI3 Gene Activates the AtHAK5prom–LUC Reporter. (A) The 8–1–24 mutant plants emit strong luminescence under K+-sufficient conditions. # 8–1–24, plants transformed with AtHAK5prom–LUC and tagged by pDKS2-7 activation vector. Negative control (NC), plants transformed with AtHAK5prom–LUC. Positive control (PC), plants transformed with 35Sprom–LUC (i.e. LUC under the control of 35S promoter). Left, bright-field image of all plants; right, luminescence image of the plants. The color scale on the right shows the luminescence intensity from dark blue (lowest) to white (highest). (B) The T-DNA inserted upstream of RCI3 (At1g05260) gene in the 8–1–24 mutant. The GenomeWalker system was used to identify the T-DNA insertion site with left border-specific primers. The distance from the T-DNA insertion site to each gene is indicated in kilobases. (C) RCI3 is overexpressed in the 8–1–24 mutant. Expression levels relative to the wild-type of two genes, At1g05260 and At1g05230, were obtained by quantitative real-time PCR in wild-type (WT), NC, and 8–1–24 mutant. Data represent the mean of three biological replicates. Molecular Plant 2010 3, 420-427DOI: (10.1093/mp/ssp121) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 2 ROS Production Is Induced in Plants Overexpressing RCI3 under Normal Conditions. (A) RT–PCR analysis of RCI3 expression in wild-type, four RCI3-ox, and rci3-1 plants. (B) ROS fluorescence images are shown in wild-type, RCI3-ox, and rci3-1 roots grown on K+-sufficient (+ K, 1.75 mM K+) or K+-deprived (–K, 0 mM K+) medium. ROS was visualized by staining 3-day-old roots with 20 μM DFFDA. Yellow and red colors indicate higher ROS production as indicated in pseudocolor scale. Inset shows enlargement of RHDZ. White lines show the boundary of roots. Bar = 100 μm. (C) Quantified data from images shown in (B). Roots were deprived K+ for 24 h. Data represent the mean ± standard deviation. Asterisks indicate a significant difference; n = 10 plants). (D) Three-day-old RCI3-ox seedlings were incubated with NADPH oxidase inhibitor (DPI) for 1 h under K+-sufficient (+K, 1.75 mM K+) conditions. Molecular Plant 2010 3, 420-427DOI: (10.1093/mp/ssp121) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 3 RCI3–YFP Protein Is Localized to ER and then Secreted to the Cell Wall. (A) Sub-cellular localization of YFP control, RCI3–YFP, and ER–CFP in Arabidopsis protoplast. Signals were detected 24 h after incubation from bright-field (bright), YFP, or CFP. (B) Sub-cellular co-localization of RCI3–YFP with ER–CFP in Arabidopsis protoplast. Expression of the genes was examined at 24 h after transformation. Signals from bright-field (bright), YFP (YFP; green), CFP (CFP: red), and merge (co-localization appears yellow). (C) Fluorescence proteins were transiently expressed in onion epidermal cells: YFP (top) and RCI3–YFP (bottom). Signals were detected 48 h after incubation from bright-field (bright), YFP (YFP), and merged images. (D) The localization of RCI3–YFP in plasmolyzed onion cells: YFP (top) and RCI3–YFP (bottom). Signals were detected 48 h after incubation from bright-field (bright), YFP (YFP), and merged images. White lines show the boundary of plasma membrane of the cell. Molecular Plant 2010 3, 420-427DOI: (10.1093/mp/ssp121) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 4 RCI3 Expression Is Induced by K+ Deprivation and Affects AtHAK5 Expression. (A) Quantitative real-time PCR analysis of RCI3 under nutrient-deprived conditions. Arabidopsis Col-0 roots were grown under N (–N), P (–P), or K+ deficiency (–K) for 6 or 30 h. Asterisks indicate a significant difference as compared to other means in each subfigure (* p < 0.05; Student's t-test). (B) AtHAK5 expression in wild-type, RCI3-ox, and rci3-1 knockout plants under K+-sufficient or K+-deprived conditions. RNA was extracted from whole plants. Data represent the mean of three biological replicates. Different letters indicate a significant difference between means at p < 0.05 (TUKEY HSD). Molecular Plant 2010 3, 420-427DOI: (10.1093/mp/ssp121) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 5 Altered RCI3 Expression Alters Primary Root Growth in K+-Sufficient but Not K+-Deprived Conditions. (A, B) Plants were grown under complete nutrient conditions for 4 d and then transferred to either full nutrient (A, +K) or a medium with no potassium (B, –K) for 7 d. (C) Primary root length of plants shown in (A) and (B) was measured. Data represent the mean ± standard deviation. Different letters indicate a significant difference between means at p < 0.05 (TUKEY HSD) (n = 70 plants). (D) The primary root-length as A percentage of full nutrient medium to medium without potassium. Data represent the mean ± standard deviation. Different letters indicate a significant difference between means at p < 0.05 (TUKEY HSD) (n = 70 plants). Molecular Plant 2010 3, 420-427DOI: (10.1093/mp/ssp121) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions