High-resolution metabolomics to identify urine biomarkers in corticosteroid-resistant asthmatic children Youngja H. Park, PhD, Anne M. Fitzpatrick, PhD,

Slides:

Advertisements

Similar presentations

Joint modeling of parentally reported and physician-confirmed wheeze identifies children with persistent troublesome wheezing Danielle C.M. Belgrave, MSc,

Advertisements

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,

Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.

Endoplasmic reticulum stress influences bronchial asthma pathogenesis by modulating nuclear factor κB activation So Ri Kim, MD, PhD, Dong Im Kim, MS, Mi.

Incidence of parentally reported and clinically diagnosed food hypersensitivity in the first year of life Carina Venter, BSc, Brett Pereira, MRCP, Jane.

D-dimer: A biomarker for antihistamine-resistant chronic urticaria

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Anne M. Fitzpatrick, PhD, MSCR, W. Gerald Teague, MD

Incidence of parentally reported and clinically diagnosed food hypersensitivity in the first year of life Carina Venter, BSc, Brett Pereira, MRCP, Jane.

Fatty acids, inflammation, and asthma

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Ann-Marie M. Schoos, MD, PhD, Jacob D

Stephen J. Fowler, MD, Gaël Tavernier, PhD, Robert Niven, MD

Is 9 more than 2 also in allergic airway inflammation?

Intramuscular Versus Intravenous Immunoglobulin Replacement Therapy and Measurement of Immunoglobulin Levels During Immunoglobulin Replacement Therapy

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD

Fatty acids, inflammation, and asthma

Correction Journal of Allergy and Clinical Immunology

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Physician needs in health informatics: Just ask the docs

Nonadherence to Asthma Treatment: Getting Unstuck

Peter M. Wolfgram, MD, David B. Allen, MD

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Jewlya Lynn, PhD, Sophie Oppenheimer, MS, MPH, Lorena Zimmer, MA

Reply Journal of Allergy and Clinical Immunology

Debra J. Palmer, PhD, Thomas R. Sullivan, BMa&CompSc(Hons), Michael S

Risk of congenital malformations for asthmatic pregnant women using a long-acting β2- agonist and inhaled corticosteroid combination versus higher-dose.

Intranasal triamcinolone use during pregnancy and the risk of adverse pregnancy outcomes Anick Bérard, MSc, PhD, Odile Sheehy, MSc, Marie-Laure Kurzinger,

Time for a paradigm shift in asthma treatment: From relieving bronchospasm to controlling systemic inflammation Leif Bjermer, MD Journal of Allergy.

Sheena D. Brown, PhD, MSCR, Katherine M. Baxter, BS, Susan T

Lung function trajectories from birth through puberty reflect asthma phenotypes with allergic comorbidity Karin C. Lødrup Carlsen, MD, PhD, Petter Mowinckel,

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

Biosimilars and drug development in allergic and immunologic diseases

Karen C. Dannemiller, PhD, Janneane F. Gent, PhD, Brian P

A cluster-randomized trial shows telephone peer coaching for parents reduces children's asthma morbidity Jane M. Garbutt, MB, ChB, Yan Yan, MD, PhD,

Claus Bachert, MD, PhD, Cezmi A. Akdis, MD

Update on the role of prostaglandins in allergic lung inflammation: Separating friends from foes, harder than you might think Martin L. Moore, PhD, R.

Altered metabolic profile in patients with IgE to galactose-alpha-1,3-galactose following in vivo food challenge John W. Steinke, PhD, Shawna L. Pochan,

What is an “eosinophilic phenotype” of asthma?

Characterization of a high TNF-α phenotype in children with moderate-to-severe asthma Sheena D. Brown, PhD, Lou Ann Brown, PhD, Susan Stephenson, PhD,

Allergenic significance of cephalosporin side chains

H. William Kelly, PharmD Journal of Allergy and Clinical Immunology

Autophagy: Nobel Prize 2016 and allergy and asthma research

Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment

Advances in the approach to the patient with food allergy

Diagnosis and management of early asthma in preschool-aged children

Geographic variability in childhood asthma prevalence in Chicago

High prevalence of severe asthma in a large random population study

A single intervention for cockroach control reduces cockroach exposure and asthma morbidity in children Felicia A. Rabito, PhD, MPH, John C. Carlson,

Mary F. Linehan, PhD, Timothy L. Frank, MD, Michelle L

Syk expression and IgE-mediated histamine release in basophils as biomarkers for predicting the clinical efficacy of omalizumab Donald W. MacGlashan,

One step forward, 2 steps back: The enigma of preschool wheeze

Levels of nitric oxide oxidation products are increased in the epithelial lining fluid of children with persistent asthma Anne M. Fitzpatrick, PhD, Lou.

Anne M. Fitzpatrick, PhD, Youngja Park, PhD, Lou Ann S

Epinephrine (adrenaline) absorption from new-generation, taste-masked sublingual tablets: A preclinical study Ousama Rachid, BPharm, MSc, Mutasem M.

Erik J. Saude, MD, PhD, Christopher D

Martin Brasholt, MD, Florent Baty, PhD, Hans Bisgaard, MD, DMSci

Reply Journal of Allergy and Clinical Immunology

Machine learning, natural language programming, and electronic health records: The next step in the artificial intelligence journey? Neil Mehta, MBBS,

Reply Journal of Allergy and Clinical Immunology

Macrolide antibiotics and asthma treatment

Asthma: The past, future, environment, and costs

Personal and parental nativity as risk factors for food sensitization

Food allergy is associated with Staphylococcus aureus colonization in children with atopic dermatitis Andrea L. Jones, MD, Douglas Curran-Everett, PhD,

Environmental factors and eosinophilic esophagitis

Natural history of cow’s milk allergy

The Editors’ Choice Journal of Allergy and Clinical Immunology

Primary prevention of asthma and allergy

Itching as a systemic disease

Presentation transcript:

High-resolution metabolomics to identify urine biomarkers in corticosteroid-resistant asthmatic children Youngja H. Park, PhD, Anne M. Fitzpatrick, PhD, Carl Angelo Medriano, MEng, Dean P. Jones, PhD Journal of Allergy and Clinical Immunology Volume 139, Issue 5, Pages 1518-1524.e4 (May 2017) DOI: 10.1016/j.jaci.2016.08.018 Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Schematic design for biomarker discovery and algorithm development. IM, Intramuscular. Journal of Allergy and Clinical Immunology 2017 139, 1518-1524.e4DOI: (10.1016/j.jaci.2016.08.018) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 A, Manhattan plot between CS-R and CS-NR children using FDR q = 0.05; 30 significant features out of 8570 features. B, HCA between CS-R and CS-NR children using the significant features. Journal of Allergy and Clinical Immunology 2017 139, 1518-1524.e4DOI: (10.1016/j.jaci.2016.08.018) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 KEGG pathway with 30 FDR q = 0.05 significant features, a total of 21 hits on KEGG human metabolic pathways. FMN, Flavin mononucleotide. Journal of Allergy and Clinical Immunology 2017 139, 1518-1524.e4DOI: (10.1016/j.jaci.2016.08.018) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Two important urine biomarkers on the GSH pathway. *Significantly different (FDR q < 0.05). Journal of Allergy and Clinical Immunology 2017 139, 1518-1524.e4DOI: (10.1016/j.jaci.2016.08.018) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 The putative biomarkers to discriminate CS-R versus CS-NR children at visit 2. *Significantly different (FDR q < 0.05). Journal of Allergy and Clinical Immunology 2017 139, 1518-1524.e4DOI: (10.1016/j.jaci.2016.08.018) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Mummichog pathway analysis with significant metabolites to determine an important module between CS-R and CS-NR children. *Significantly different (FDR q < 0.05). Journal of Allergy and Clinical Immunology 2017 139, 1518-1524.e4DOI: (10.1016/j.jaci.2016.08.018) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Orthogonal signal correction/partial least square-discriminant analysis on children with mild-to-moderate and severe asthma. Blue, those with mild-to-moderate asthma; Red, those with severe asthma. Journal of Allergy and Clinical Immunology 2017 139, 1518-1524.e4DOI: (10.1016/j.jaci.2016.08.018) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Left, Manhattan plot between children with mild-to-moderate and children with severe asthma using FDR q = 0.05; 119 significant features out of 6963 features. Right, HCA between children with mild-to-moderate and children with severe asthma using the significant features. Journal of Allergy and Clinical Immunology 2017 139, 1518-1524.e4DOI: (10.1016/j.jaci.2016.08.018) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 KEGG pathway with FDR q = 0.05 significant features, a total of 38 hits on KEGG human metabolic pathways between children with mild-to-moderate and children with severe asthma. Journal of Allergy and Clinical Immunology 2017 139, 1518-1524.e4DOI: (10.1016/j.jaci.2016.08.018) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 Bar graph of the compounds between children with mild-to-moderate and children with severe asthma. Journal of Allergy and Clinical Immunology 2017 139, 1518-1524.e4DOI: (10.1016/j.jaci.2016.08.018) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions