Evidence for the dysregulated expression of TWIST1, TGFβ1 and SMAD3 in differentiating osteoblasts from primary hip osteoarthritis patients D.D. Kumarasinghe,

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Presentation transcript:

Evidence for the dysregulated expression of TWIST1, TGFβ1 and SMAD3 in differentiating osteoblasts from primary hip osteoarthritis patients D.D. Kumarasinghe, T. Sullivan, J.S. Kuliwaba, N.L. Fazzalari, G.J. Atkins Osteoarthritis and Cartilage Volume 20, Issue 11, Pages 1357-1366 (November 2012) DOI: 10.1016/j.joca.2012.07.005 Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 (A) Alizarin red stains from primary osteoblast cultures differentiated over 42 days generated from the IT trabecular bone of a female hip OA case and a CTL. (B) Calcium apposition generated from quantified alizarin red stains from primary osteoblast cultures differentiated over 42 days generated from the IT trabecular bone of age and sex-matched female hip OA and CTL cases, values are means ± standard error of the mean (s.e.m.) of quadruplicate stains. (C) Images produced by scanning electron microscopy at 2000× magnification from primary osteoblast cultures differentiated over 42 days generated from the IT trabecular bone of a female hip OA case and a CTL. (D) Ca:P ratios assessed using energy-dispersive x-ray microanalysis from primary osteoblast cultures differentiated over 42 days generated from the IT trabecular bone of age and sex-matched female hip OA and CTL cases, values are means ± s.e.m. of quadruplicate scans. Osteoarthritis and Cartilage 2012 20, 1357-1366DOI: (10.1016/j.joca.2012.07.005) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 mRNA expression from primary osteoblast cultures differentiated over 42 days generated from the IT trabecular bone of age and sex-matched female hip OA and CTL cases. Values are means ± s.e.m. of triplicate reactions and normalised to GAPDH (A) & (B) TNAP, (C) & (D) OCN, (E) & (F) TWIST1, (G) & (H) WNT5B, (I) & (J) TGFβ1, (K) & (L) SMAD3. Osteoarthritis and Cartilage 2012 20, 1357-1366DOI: (10.1016/j.joca.2012.07.005) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions