Scoring human sperm morphology using Testsimplets and Diff-Quik slides

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Presentation transcript:

Scoring human sperm morphology using Testsimplets and Diff-Quik slides Ilaria Natali, Ph.D., Monica Muratori, Ph.D., Valentina Sarli, B.S., Mario Vannuccini, Ph.D., Sonia Cipriani, Sc.D., Laura Niccoli, M.D., Claudia Giachini, Ph.D. Fertility and Sterility Volume 99, Issue 5, Pages 1227-1232.e2 (April 2013) DOI: 10.1016/j.fertnstert.2012.11.047 Copyright © 2013 Terms and Conditions

Figure 1 Stained smears of human sperm obtained using (A) Diff-Quik and (B) Testsimplets. Fertility and Sterility 2013 99, 1227-1232.e2DOI: (10.1016/j.fertnstert.2012.11.047) Copyright © 2013 Terms and Conditions

Figure 2 Deming's regression analysis for (A) normal sperm morphology and (B) sperm head defects determined by Diff-Quik (DQ) and Testsimplets (TS) staining methods. Fertility and Sterility 2013 99, 1227-1232.e2DOI: (10.1016/j.fertnstert.2012.11.047) Copyright © 2013 Terms and Conditions

Figure 3 Bland-Altman plot of the difference versus the average of the values obtained by Diff-Quik (DQ) and Testsimplets (TS) for (A) sperm morphology and (B) head defect assessment. The continuous line (bias) represents the mean difference, and dashed lines represent the 95% limits of agreement (±1.96 standard deviation). Fertility and Sterility 2013 99, 1227-1232.e2DOI: (10.1016/j.fertnstert.2012.11.047) Copyright © 2013 Terms and Conditions

Supplemental Figure 1 Distribution of (A) normal sperm morphology and (B) head defects, percentages obtained by the Diff-Quik and Testsimplets staining methods. Fertility and Sterility 2013 99, 1227-1232.e2DOI: (10.1016/j.fertnstert.2012.11.047) Copyright © 2013 Terms and Conditions