Induction of proteinases in the human preovulatory follicle of the menstrual cycle by human chorionic gonadotropin Katherine L. Rosewell, B.A., Linah.

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Presentation transcript:

Induction of proteinases in the human preovulatory follicle of the menstrual cycle by human chorionic gonadotropin Katherine L. Rosewell, B.A., Linah Al-Alem, Ph.D., Farnosh Zakerkish, M.D., Lauren McCord, B.S., James W. Akin, M.D., Charles L. Chaffin, Ph.D., Mats Brännström, M.D., Ph.D., Thomas E. Curry, Ph.D. Fertility and Sterility Volume 103, Issue 3, Pages 826-833 (March 2015) DOI: 10.1016/j.fertnstert.2014.11.017 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 1 MMP and ADAMTS mRNA expression in human granulosa and theca cells from timed follicles collected across the periovulatory period. (A) MMP19, (B) MMP1, (C) ADAMTS1, (D) ADAMTS9 (n = 5 per group). Different letters or numbers denote significant differences between groups (P<.05). Fertility and Sterility 2015 103, 826-833DOI: (10.1016/j.fertnstert.2014.11.017) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Immunohistochemical localization of MMP and ADAMTS expression in follicles collected during the early (A, C, E, G, I, K) and late (B, D, F, H, J, L) ovulatory time periods (n = 4–5 per group). (A, B) MMP19; (C, D) MMP1; (E, F) ADAMTS1; (G, H) ADAMTS9; (I, J) no primary antibody. Low-magnification photomicrographs of the unstained follicle with a red box indicating where the proceeding photomicrographs were taken (K, L). MMP and ADAMTS proteins were visualized by the chromogen Vulcan red; hematoxylin was used as the counterstain. Bars indicate 50 μm, except in panel K, where the bar indicates 100 μm. Panel L is a composite of four pictures taken at ×4 magnification. G = granulosa layer; T = theca layer. Fertility and Sterility 2015 103, 826-833DOI: (10.1016/j.fertnstert.2014.11.017) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Induction of MMP and ADAMTs mRNA expression in human granulosa-lutein cells from IVF patients. Granulosa-lutein cells were cultured for 6–7 days and stimulated with hCG, and mRNA expression was examined at 6, 12, and 24 hours (n = 6). Different letters denote significant differences between groups (P<.05). (A) MMP19, (B) MMP1, (C) ADAMTS1, (D) ADAMTS9. Fertility and Sterility 2015 103, 826-833DOI: (10.1016/j.fertnstert.2014.11.017) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 4 MMP and ADAMTS mRNA expression in HGL5 cells cultured in the presence or absence of FSK plus PMA for up to 24 hours (n = 3). Different letters denote significant differences between groups (P<.05). (A) MMP19, (B) MMP1, (C) ADAMTS1, (D) ADAMTS9. Fertility and Sterility 2015 103, 826-833DOI: (10.1016/j.fertnstert.2014.11.017) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions