Prognostic Gene Expression Signatures Can Be Measured in Tissues Collected in RNAlater Preservative Dondapati Chowdary, Jessica Lathrop, Joanne Skelton,

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Presentation transcript:

Prognostic Gene Expression Signatures Can Be Measured in Tissues Collected in RNAlater Preservative Dondapati Chowdary, Jessica Lathrop, Joanne Skelton, Kathleen Curtin, Thomas Briggs, Yi Zhang, Jack Yu, Yixin Wang, Abhijit Mazumder The Journal of Molecular Diagnostics Volume 8, Issue 1, Pages 31-39 (February 2006) DOI: 10.2353/jmoldx.2006.050056 Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Box plot depicting the distribution in the Pearson correlation coefficients obtained from the 6 colon tumor samples performed in triplicate (18 pairwise comparisons), 34 pairwise comparisons for the lymph node-negative breast tumors (including repeats), and 25 pairwise comparisons of Dukes' B colon tumors (including repeats). The line in the box indicates the median whereas the edges of the box indicate the 25th and 75th percentiles of the data. The Journal of Molecular Diagnostics 2006 8, 31-39DOI: (10.2353/jmoldx.2006.050056) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Box plot depicting the distribution in the M values obtained from the 18 pairwise comparisons of the colon tumor replicates (A), 34 pairwise comparisons for the RNAlater preservative-suspended and snap-frozen breast tumors (B), and 25 pairwise comparisons of RNAlater preservative-suspended and snap-frozen colon tumors (C). The line in the box indicates the median whereas the edges of the box indicate the 25th and 75th percentiles of the data. The y axis is a log2 scale. M is defined as the log2 (intensity1/intensity2). Three comparisons were repeated for the breast tumors and four comparisons for the colon tumors. The Journal of Molecular Diagnostics 2006 8, 31-39DOI: (10.2353/jmoldx.2006.050056) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 A: Hierarchical clustering for the 52 breast samples (26 snap-frozen and 26 RNAlater preservative-suspended). B: Hierarchical clustering for the 44 colon samples (22 snap-frozen and 22 RNAlater preservative-suspended, including repeats). Each column represents one sample, and each row represents one gene. The sample type (RNAlater-preserved or snap-frozen) is coded by color and by the letter R (for RNAlater-preserved) or T (for snap-frozen). The Journal of Molecular Diagnostics 2006 8, 31-39DOI: (10.2353/jmoldx.2006.050056) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions