CD8+ T cells from lupus-prone mice demonstrate substantial oligoclonality. CD8+ T cells from lupus-prone mice demonstrate substantial oligoclonality. (A–D)

Slides:

Advertisements

Similar presentations

T-cell clonality assessment by next-generation sequencing improves detection sensitivity in mycosis fungoides Kari E. Sufficool, MD, Christina M. Lockwood,

Advertisements

GC TFH cells exhibit HIV antigen–driven clonal expansion and selection

Thymic function in MHC class II–deficient patients

Clonal bias of CD56−CD16+ NK cell subpopulations.

Blood T-cell receptor diversity decreases during the course of HIV infection, but the potential for a diverse repertoire persists by Paul D. Baum, Jennifer.

by Samuel D. Perli, Cheryl H. Cui, and Timothy K. Lu

Tiago R. Matos, Menno A. de Rie, Marcel B.M. Teunissen

Initial T Cell Receptor Transgenic Cell Precursor Frequency Dictates Critical Aspects of the CD8+ T Cell Response to Infection Vladimir P. Badovinac,

Dual-Luciferase Activation Assay with Potential Targets of ZmbZIP22.

IgA and IgM VH repertoires in human colon: Evidence for clonally expanded B cells that are widely disseminated Wolfgang Holtmeier, Andreas Hennemann,

Treg and Teff cell subsets show increased TCR overlap during colitis.

Thrombopoietin responsiveness reflects the number of doublings undergone by megakaryocyte progenitors by Jean-Michel Paulus, Najet Debili, Frédéric Larbret,

MP cells are generated from naïve cells in the periphery.

T-cell clonality assessment by next-generation sequencing improves detection sensitivity in mycosis fungoides Kari E. Sufficool, MD, Christina M. Lockwood,

Expansion of Bacteroides species during colitis does not enhance TCR-specific T cell responses. Expansion of Bacteroides species during colitis does not.

Identification of checkpoints in human T-cell development using severe combined immunodeficiency stem cells Anna-Sophia Wiekmeijer, MSc, Karin Pike-Overzet,

nramp2 Mutants Are Hypersensitive to Mn Deficiency.

Volume 4, Issue 1, Pages (January 2015)

Variable CDR3 lengths support the notion of oligoclonality in CD8+ T cells of lupus-prone mice. Variable CDR3 lengths support the notion of oligoclonality.

The CD8+ T cell repertoire is largely similar between the spleen and brain of the same lupus-prone animal. The CD8+ T cell repertoire is largely similar.

Regions of sequence homology across dominant clones in TLR7tg animals.

Isolating CD8+ T cells from lupus-prone mice.

Highly variable VDJβ gene usage in lupus-prone CD8+ T cells.

Example: Calculate how many squares altogether (2 x 5 = 10)

Neoantigen-specific TCR expansion in stimulated T-cell cultures.

Volume 42, Issue 5, Pages (May 2015)

Volume 12, Issue 4, Pages (April 2000)

The adenylate cyclase toxin of Bacillus anthracis is a potent promoter of TH17 cell development Silvia Rossi Paccani, PhD, Marisa Benagiano, PhD, Maria.

Volume 31, Issue 3, Pages (September 2009)

IBD development correlates with peripheral T cell activation in DNR mice. IBD development correlates with peripheral T cell activation in DNR mice. (A)

Pritam Chanda, Aidong Zhang, Daniel Brazeau, Lara Sucheston, Jo L

IL-10 upregulates IgG4 production by CD27+ B cells.

Kevin G. Haworth, Christina Ironside, Zachary K. Norgaard, Willimark M

Katelyn T. Byrne, Robert H. Vonderheide Cell Reports

The Shaping of the T Cell Repertoire

by Justin J. Taylor, Kathryn A. Pape, Holly R. Steach, and Marc K

Arp2/3-mediated formation of nuclear actin networks is essential for CD4+ T cell effector functions. Arp2/3-mediated formation of nuclear actin networks.

Katelyn T. Byrne, Robert H. Vonderheide Cell Reports

Location, morphology and gene expression of skin CD8α+MHC II+ cells.

CD4-TEMRA cells show marked clonal expansion.

MP cells consist of rapidly proliferating CD5hi and relatively quiescent CD5lo cells, both of which require CD28 signaling for Ki67 expression. MP cells.

Kinetics of clone appearance, size, persistence, and lineage content.

MP cells are generated from naïve cells in the periphery.

GPR55 restrains IEL accumulation in the small intestine.

Dominance of Nonmalignant T-Cell Clones and Distortion of the TCR Repertoire in the Peripheral Blood of Patients with Cutaneous CD30+ Lymphoproliferative.

Novel presentation of Omenn syndrome in association with aniridia

PMPs survive STZ cytotoxicity and produce larger colonies with a higher yield of insulin+ progeny. PMPs survive STZ cytotoxicity and produce larger colonies.

Figure 4 Vα7.2 TCR chain repertoire Analysis of the T-cell receptor (TCR) Vα7.2 repertoire of patient A by pyrosequencing shows oligoclonal T-cell expansions.

CD4-TEMRA cells are heterogeneous across donors.

CD4+CLA+CD103+ T cells in skin and blood are clonally related.

MR1T clones with distinct TCR usage display differential recognition of discrete activating ligands. MR1T clones with distinct TCR usage display differential.

CPI-444 efficacy requires CD8+ T cells and is associated with increased CD73 expression. CPI-444 efficacy requires CD8+ T cells and is associated with.

Saccharification yields.

Fig. 3 NK cells are enriched in ICB-sensitive tumors in mouse models and patients and are required for response. NK cells are enriched in ICB-sensitive.

MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. PBMCs.

Ovalbumin-specific IgE modulates ovalbumin-specific T-cell response after repetitive oral antigen administration Nemuko Omata, MD, Yusei Ohshima, MD,

HRI depletion elevates γ-globin in primary erythroid CD34+ cells

Fig. 2 Expressions of cll, abd-A, and Abd-B by qRT-PCR.

Evaluation of top candidate clones for selective killing in bispecific antibody format. Evaluation of top candidate clones for selective killing in bispecific.

FOXO1 inhibits Runx2-induced invasion of prostate cancer cells.

CD4-CTL effectors share TCR clonotypes with CD4-CTL precursors.

ESKM treatment does not affect leukocyte or HSC counts in HLA-A

Activation status of CD8+ T cells.

Ten most frequent TCRs in the bulk 12TILs comprise >99% of the TIL population, and the neoantigens are shown to be the most dominant clones. Ten most frequent.

Mutation of the Smad3 linker phosphorylation sites in 4T1 cells reduces putative stem cell population and tumor initiating frequency of cells from the.

Integrated analysis of gene expression and copy number alterations.

AXL knockout does not prevent dormancy.

TRP1 transnuclear CD8 T cells are tumoricidal in vitro.

Cxxc1-deficient TH17 cells exhibit a Treg cell–like expression profile

Presentation transcript:

CD8+ T cells from lupus-prone mice demonstrate substantial oligoclonality. CD8+ T cells from lupus-prone mice demonstrate substantial oligoclonality. (A–D) Overview of total TCRβ rearrangements from wild type B6 and TLR7tg CD8+ T cells from Fig. 1. (A) The count of unique rearrangements in each sample that are in-frame and do not contain a stop codon. (B) The sum of templates for all productive rearrangements in the sample. (C) Clonality measure for each sample calculated over all productive rearrangements. (D) The fraction of productive in-frame templates among all templates. (E–G) The frequency (E) and sum (F) of the top 10 clones and the top clone (G) for each sample relative to all rearrangements. Error bars indicate mean + SD. ^p = 0.08, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ***p ≤ 0.0001, by Student t test. Peter A. Morawski, and Silvia Bolland ImmunoHorizons 2019;3:61-70 ©2019 by American Association of Immunologists