Fertility and Sterility Differences in mitochondrial and nuclear DNA status of high-density and low-density sperm fractions after density centrifugation preparation  Michael O’Connell, Ph.D., Neil McClure, M.D., Lesley A Powell, Ph.D., E.Kristine Steele, M.D., Sheena E.M Lewis, Ph.D.  Fertility and Sterility  Volume 79, Pages 754-762 (March 2003) DOI: 10.1016/S0015-0282(02)04827-6

FIGURE 1 Long polymerase chain reaction mitochondrial DNA fragments in high-density (A) and low-density (B) fractions. M = marker; C = control. O’Connell. Sperm DNA after Percoll. Fertil Steril 2003. Fertility and Sterility 2003 79, 754-762DOI: (10.1016/S0015-0282(02)04827-6)

FIGURE 2 Incidence of single, double and multiple deletions in high-density and low-density fractions. ■ 90% fraction; 45% fraction. All mitochondrial DNA fragments for each fraction add up to 100%. WT = wild type. O’Connell. Sperm DNA after Percoll. Fertil Steril 2003. Fertility and Sterility 2003 79, 754-762DOI: (10.1016/S0015-0282(02)04827-6)

FIGURE 3 Mitochondrial deletion size in high-density and low-density fractions. ■ 90% fraction; 45% fraction. All mitochondrial DNA fragments for each fraction add up to 100%. WT = wild type. O’Connell. Sperm DNA after Percoll. Fertil Steril 2003. Fertility and Sterility 2003 79, 754-762DOI: (10.1016/S0015-0282(02)04827-6)

FIGURE 4 Nuclear DNA fragmentation in high-density and low-density fractions. ■ 90% fraction; 45% fraction. Values are means (±SE, as indicated by the bars). *P<.05, Student t-test for paired samples. O’Connell. Sperm DNA after Percoll. Fertil Steril 2003. Fertility and Sterility 2003 79, 754-762DOI: (10.1016/S0015-0282(02)04827-6)