Mammalian Fetal Cardiac Regeneration After Myocardial Infarction Is Associated With Differential Gene Expression Compared With the Adult Carlos Zgheib,

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Presentation transcript:

Mammalian Fetal Cardiac Regeneration After Myocardial Infarction Is Associated With Differential Gene Expression Compared With the Adult Carlos Zgheib, PhD, Myron W. Allukian, MD, Junwang Xu, PhD, Michael W. Morris, MD, Robert C. Caskey, MD, Benjamin J. Herdrich, MD, Junyi Hu, MD, Joseph H. Gorman, MD, Robert C. Gorman, MD, Kenneth W. Liechty, MD The Annals of Thoracic Surgery Volume 97, Issue 5, Pages 1643-1650 (May 2014) DOI: 10.1016/j.athoracsur.2014.01.013 Copyright © 2014 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 The principal component analysis two-dimensional scatter plot represents the differential gene expression patterns of infarct and remote zone of adult and fetal hearts at 3 and 30 days after myocardial infarction. Open markers correspond to fetal heart, and closed markers correspond to adult heart. Box markers correspond to 3 days after infarction, and diamond markers correspond to 30 days after infarction. Blue indicates remote, and red indicates infarct. (PC1 = principal component analysis component 1 [32.2% variance]; PC2 = principal component analysis component 2 [23.7% variance].) The Annals of Thoracic Surgery 2014 97, 1643-1650DOI: (10.1016/j.athoracsur.2014.01.013) Copyright © 2014 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Violin plots for the genes related to the gene ontology term response to wounding. The y-axis represents the log2 of the ratio of the infarct to remote region average gene expression. The violin shapes represent the distribution of the log2 ratios in each group. The adult infarcts demonstrated increased expression of response to wounding genes at day 3 and persistence of the expression at 30 days, whereas the fetal infarct gene expression of response to wounding genes returned to baseline by 30 days (29 genes; p < 0.005, Student's t test). The Annals of Thoracic Surgery 2014 97, 1643-1650DOI: (10.1016/j.athoracsur.2014.01.013) Copyright © 2014 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 (A) Violin plots for the genes related to the gene ontology term inflammatory response. The y-axis represents the log2 of the ratio of the infarct to remote region average gene expression. The violin shapes represent the distribution of the log2 ratios in each group. The adult infarcts demonstrated increased expression of inflammatory response genes at day 3 and persistence of the expression at 30 days, whereas the fetal infarct gene expression of inflammatory response genes returned to baseline by 30 days (162 genes; p < 0.005, Student's t test). (B) Real-time quantitative polymerase chain reaction analysis of mRNA for interleukin 6 (IL-6) and interleukin 8 (IL-8) in fetal and adult hearts at 3 and 30 days after myocardial infarction. (I = infarct; RM = remote.) The Annals of Thoracic Surgery 2014 97, 1643-1650DOI: (10.1016/j.athoracsur.2014.01.013) Copyright © 2014 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 Violin plots for the genes related to the gene ontology term extracellular matrix. The y-axis represents the log2 of the ratio of the infarct to remote region average gene expression. The violin shapes represent the distribution of the log2 ratios in each group. The adult infarcts demonstrated increased expression of extracellular matrix genes at day 3 and persistence of the expression at 30 days, whereas the fetal infarct gene expression of extracellular matrix genes returned to baseline by 30 days (20 genes; p < 0.005, Student's t test). The Annals of Thoracic Surgery 2014 97, 1643-1650DOI: (10.1016/j.athoracsur.2014.01.013) Copyright © 2014 The Society of Thoracic Surgeons Terms and Conditions

Fig 5 Violin plots for the genes related to the GO term: (A) cell cycle (254 genes), (B) proliferation (161 genes) and (C) migration (33 genes). The y-axis represents the log2 of the ratio of the infarct to remote region average gene expression. The violin shapes represent the distribution of the log2 ratios in each group. The adult infarcts demonstrated increased expression of cell cycle, proliferation and migration genes at day 3 and persistence of the expression at 30 days, whereas the fetal infarct gene expression of response to wounding genes returned to baseline by 30 days (p < 0.005, Student's t test). The Annals of Thoracic Surgery 2014 97, 1643-1650DOI: (10.1016/j.athoracsur.2014.01.013) Copyright © 2014 The Society of Thoracic Surgeons Terms and Conditions

Fig 6 Violin plots for the genes related to the gene ontology term apoptosis. The y-axis represents the log2 of the ratio of the infarct to remote region average gene expression. The violin shapes represent the distribution of the log2 ratios in each group. The adult infarcts demonstrated increased expression of apoptosis genes at day 3 and persistence of the expression at 30 days, whereas the fetal infarct gene expression of apoptosis genes returned to baseline by 30 days (235 genes; p < 0.005, Student's t test). The Annals of Thoracic Surgery 2014 97, 1643-1650DOI: (10.1016/j.athoracsur.2014.01.013) Copyright © 2014 The Society of Thoracic Surgeons Terms and Conditions