DNA biosensor as a confirmatory test: Studies of household cleaners effects of onto electrochemical DNA detection Oliveira N.C.L. , Campos-Ferreira D.S.

Slides:

Advertisements

Similar presentations

The most efficient STR loci in forensic genetics in population of central Poland R. Jacewicz, M. Jedrzejczyk, J. Berent Forensic Science International:

Advertisements

B. Szkuta, M.L. Harvey, K.N. Ballantyne, R.A.H. van Oorschot

On the effect of shooting distance, ballistic model construction, doping and weapon type on the simultaneous analysis of DNA and RNA from backspatter.

S. Cisana, A. Verzeletti, F. Vassanelli, L. Bontempi, N. Cerri, V

A. Verzeletti, V. Cortellini, S. Cisana, S. Pretto, N. Cerri, F

Activity level in the hierarchy of propositions in case of the People of the State of California v. Orenthal James Simpson analyzed using Bayesian network

Use of matrix standards for new fluorophores in capillary sequencers

PhyloTree Build 17: Growing the human mitochondrial DNA tree

Performance of the RapidHIT™200

A. Barbaro, P. Cormaci, G. Falcone

Use of universal reporter primers in multiplex PCR of autosomal loci

Forensic identification of 12 mammals species based on size variation of mitochondrial cytochrome b gene using multiplex PCR assay M. Črček, K. Drobnič

Probabilistic modelling for DNA mixture analysis

C. Davies, J. Thomson, F. Kennedy

Naoto Iizuka, Ryo Kobayashi, Yukio Itoh

E.M. Dauber, B. Glock, W.R. Mayr

The effect of increased cycle numbers using the Quantifiler® Duo DNA Quantification Kit (AB) on the detection of minute amounts of male DNA in mixtures.

Two mothers and two fathers for two half-brothers’ pairs

DNA typing of trace DNA recovered from different areas of sandals found at a homicide crime scene investigation: A comparative study Samuel T.G. Ferreira,

Juliana G. Aquino, Juliana Jannuzzi, Elizeu F. Carvalho, Leonor Gusmão

A comparative study between muscle, cartilage and swab from inside the urinary bladder samples for DNA typing of severely burnt bodies in disaster victim.

Establishment of Italian national DNA database and the central laboratory: Some aspects R. Biondo, F. De Stefano Forensic Science International: Genetics.

Timothy J. Verdon, R. John Mitchell, Roland A.H. van Oorschot

Effectiveness of CIFS DNA database in Thailand

U. Ricci, I. Carboni, S. Iozzi, A. Gozzini, F. Torricelli

A custom software solution for forensic mtDNA analysis of MiSeq data

R. Alghafri, S. Alhammadi, K. Amiri

Population data for SE33 locus in United Arab Emirates Arab population

S. Iyavoo, A. Wolejko, D. Furmanczyk, R. Graham, R. Myers, T. Haizel

Automated extraction of DNA from clothing

Combining DNA evidence for greater match information

Andréa M. Oliveira, Leonor Gusmão, Peter M. Schneider, Iva Gomes

False homozygosity at D12S391 locus: A case report

A. Verzeletti, V. Cortellini, S. Cisana, S. Pretto, N. Cerri, F

A. Trindade-Filho, C. Mendes, S. Ferreira, S. Oliveira, A

P. Dario, A. R. Oliveira, T. Ribeiro, M. J. Porto, J. Costa Santos, D

Efficient DNA extraction from hair shafts

Evaluating DNA profiles using peak heights, allowing for dropin, dropout and stutters Roberto Puch-Solis Forensic Science International: Genetics Supplement.

Multiplex DNA amplification and barcoding in a single reaction for 454 Roche sequencing: A comprehensive study on the control region of the mitochondrial.

Distribution of Y chromosome haplogroup Q in Greenlanders

Analysis of forensic samples in Banco Nacional de Datos Genéticos

Introducing a latent variable approach for finding populations in a forensic DNA database Maarten Kruijver Forensic Science International: Genetics.

Determination of siblings: A special case report from Halle

C.J. Bruijning-van Dongen, K. Slooten, W. Burgers, W. Wiegerinck

Developmental validation of a fully automated genotyping assay capable of detecting length and sequence variation in the CODIS STR loci David D. Duncan,

Testing the behavior of GlobalFiler® PCR amplification kit with degraded and/or inhibited biological samples V. Bogas, M. Carvalho, F. Corte-Real, M.J.

M. Hara, T. Masuda, A. Takada, T. Miyazaki, H. Suzuki, A. Kido, K

Is an increased drop-in rate appropriate with enhanced DNA profiling?

Semen detection: A retrospective overview from 2010

Application of less primer method to commercial kits

Linda Albinsson, Johannes Hedman, Ricky Ansell

DNA persistance in soft tissues exposed to extreme environments

Eye colour and SNPs in Danes

Kpop: A Python package for population genetics analysis

Simulated approach to estimate the number and combination of known/unknown contributors in mixed DNA samples using 15 short tandem repeat loci Sho Manabe,

Sho Manabe, Yuya Hamano, Chihiro Kawai, Chie Morimoto, Keiji Tamaki

Monique Wesselink, Irene Kuiper

Influence of the luminol chemiluminescence reaction on the confirmatory tests for the detection and characterization of bloodstains in forensic analysis

Effects of the most common methods for the enhancement of latent fingerprints on DNA extraction from forensic samples S. Gino, M. Omedei Forensic Science.

Founding mothers of Chueta population

Improved performance for forensic casework: Extraction and isolation updates for the Maxwell® 16 instrument M. Lindner, P.V. Mandrekar, J. Bessetti,

Development of a 24-plex Y chromosomal STR loci typing system

Statistical aspects of familial searching

L. Zha, L.B. Yun, H.B. Luo, J. Yan, Y.P. Hou

Andreas O. Tillmar, Helena Nilsson, Gunilla Holmlund

N. C. L. Oliveira, M. H. F. Ekert, C. A. Souza, K. C. N. Rabêlo, S. M

Forensic nomenclature for short tandem repeats updated for sequencing

First application of the Investigator DIPplex indels typing kit for the analysis of ancient DNA samples C. Hollard, F. Mendisco, C. Keyser, E. Crubézy,

Discrepancies between forensic DNA databases

A.M. Ölçen, G. Filoğlu, H. Altunçul, S. Erdem, Ö. Bülbül

Presentation transcript:

DNA biosensor as a confirmatory test: Studies of household cleaners effects of onto electrochemical DNA detection Oliveira N.C.L. , Campos-Ferreira D.S. , Nascimento G.A. , Zanforlin D.M.L. , Bezerra W.S.R. , Santos S.M. , Lima-Filho J.L. Forensic Science International: Genetics Supplement Series Volume 5, Pages e162-e164 (December 2015) DOI: 10.1016/j.fsigss.2015.09.065 Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Forensic Science International: Genetics Supplement Series 2015 5, e162-e164DOI: (10.1016/j.fsigss.2015.09.065) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Electrochemical signals obtained from differential pulse voltammetry (DPV) based on guanine oxidation on probe-modified PGE before and after hybridization with complementary sequence. Conditions: scan rate: 20mV/s, scanning potential range: +0.5V to +1.2V. Forensic Science International: Genetics Supplement Series 2015 5, e162-e164DOI: (10.1016/j.fsigss.2015.09.065) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 (A) Differential pulse voltammograms for guanine oxidation in different situations, regarding on (a) probe-modified PGE, (b) probe-modified PGE with complementary sequence, (c) probe-modified PGE with complementary sequence mixed with ultrapure water (blank solution), (d) alcohol, (e) SDS and (f) sodium hypochlorite solution. (B) Zoom in current peak region of 0–40nA, exhibiting a clear distinction among probe-modified PGE with complementary sequence (a) mixed with blank solution (b) and sodium hypochlorite solution (c). Conditions: scan rate: 20mV/s, scanning potential range: +0.5V to +1.2V. Forensic Science International: Genetics Supplement Series 2015 5, e162-e164DOI: (10.1016/j.fsigss.2015.09.065) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions